Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids
Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02035/full |
| _version_ | 1818128378227589120 |
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| author | Jinxin Liu Jinxin Liu Zhe Zhao Zhe Zhao Yiqing Deng Yan Shi Yupeng Liu Chao Wu Peng Luo Chaoqun Hu |
| author_facet | Jinxin Liu Jinxin Liu Zhe Zhao Zhe Zhao Yiqing Deng Yan Shi Yupeng Liu Chao Wu Peng Luo Chaoqun Hu |
| author_sort | Jinxin Liu |
| collection | DOAJ |
| description | Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp. is currently lacking. In this study, the complete genome of a V. campbellii predominant strain, LMB29, was determined by MiSeq in conjunction with PacBio SMRT sequencing. This genome consists of two circular DNA chromosomes and four megaplasmids. Comparative genome analysis indicates that LMB29 shares a 96.66% similarity (average nucleotide identity) with the V. campbellii ATCC strain BAA-1116 based on a 75% AF (average fraction) calculations, and its functional profile is very similar to V. campbellii E1 and V. campbellii CAIM115. Both type III secretion system (T3SS) and type VI secretion system (T6SS), along with the tlh gene which encodes a thermolabile hemolysin, are present in LMB29 which may contribute to the bacterial pathogenesis. The virulence of this strain was experimental confirmed by performing a LDH assay on a fish cell infection model, and cell death was observed as early as within 3 h post infection. Thirty-seven antimicrobial resistance genes (>45% identity) were predicted in LMB29 which includes a novel rifampicin ADP ribosyltransferase, arr-9, in plasmid pLMB157. The gene arr-9 was predicted on a genomic island with horizontal transferable potentials which may facilitate the rifampicin resistance dissemination. Future researches are needed to explore the pathogenesis of V. campbellii LMB29, but the availability of this genome sequence will certainly aid as a basis for further analysis. |
| first_indexed | 2024-12-11T07:32:18Z |
| format | Article |
| id | doaj.art-7610f9f7eb4742fea618dfa00c65c461 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T07:32:18Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-7610f9f7eb4742fea618dfa00c65c4612022-12-22T01:15:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-10-01810.3389/fmicb.2017.02035290197Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native MegaplasmidsJinxin Liu0Jinxin Liu1Zhe Zhao2Zhe Zhao3Yiqing Deng4Yan Shi5Yupeng Liu6Chao Wu7Peng Luo8Chaoqun Hu9Institute of Marine Biology, College of Oceanography, Hohai University, Nanjing, ChinaDepartment of Food Science and Technology, University of California, Davis, Davis, CA, United StatesInstitute of Marine Biology, College of Oceanography, Hohai University, Nanjing, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaInstitute of Marine Biology, College of Oceanography, Hohai University, Nanjing, ChinaInstitute of Marine Biology, College of Oceanography, Hohai University, Nanjing, ChinaInstitute of Marine Biology, College of Oceanography, Hohai University, Nanjing, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaKey Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaVibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp. is currently lacking. In this study, the complete genome of a V. campbellii predominant strain, LMB29, was determined by MiSeq in conjunction with PacBio SMRT sequencing. This genome consists of two circular DNA chromosomes and four megaplasmids. Comparative genome analysis indicates that LMB29 shares a 96.66% similarity (average nucleotide identity) with the V. campbellii ATCC strain BAA-1116 based on a 75% AF (average fraction) calculations, and its functional profile is very similar to V. campbellii E1 and V. campbellii CAIM115. Both type III secretion system (T3SS) and type VI secretion system (T6SS), along with the tlh gene which encodes a thermolabile hemolysin, are present in LMB29 which may contribute to the bacterial pathogenesis. The virulence of this strain was experimental confirmed by performing a LDH assay on a fish cell infection model, and cell death was observed as early as within 3 h post infection. Thirty-seven antimicrobial resistance genes (>45% identity) were predicted in LMB29 which includes a novel rifampicin ADP ribosyltransferase, arr-9, in plasmid pLMB157. The gene arr-9 was predicted on a genomic island with horizontal transferable potentials which may facilitate the rifampicin resistance dissemination. Future researches are needed to explore the pathogenesis of V. campbellii LMB29, but the availability of this genome sequence will certainly aid as a basis for further analysis.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02035/fullVibrio campbelliiwhole genome sequencingT6SSrifampicin resistancearr-9virulence |
| spellingShingle | Jinxin Liu Jinxin Liu Zhe Zhao Zhe Zhao Yiqing Deng Yan Shi Yupeng Liu Chao Wu Peng Luo Chaoqun Hu Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids Frontiers in Microbiology Vibrio campbellii whole genome sequencing T6SS rifampicin resistance arr-9 virulence |
| title | Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
| title_full | Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
| title_fullStr | Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
| title_full_unstemmed | Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
| title_short | Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
| title_sort | complete genome sequence of vibrio campbellii lmb 29 isolated from red drum with four native megaplasmids |
| topic | Vibrio campbellii whole genome sequencing T6SS rifampicin resistance arr-9 virulence |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02035/full |
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