Conformational Consequences for Compatible Osmolytes on Thermal Denaturation
Compatible osmolytes are a broad class of small organic molecules employed by living systems to combat environmental stress by enhancing the native protein structure. The molecular features that make for a superior biopreservation remain elusive. Through the use of time-resolved and steady-state spe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/12/1394 |
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| author | Nimesh Shukla Brianna Bembenek Erika A. Taylor Christina M. Othon |
| author_facet | Nimesh Shukla Brianna Bembenek Erika A. Taylor Christina M. Othon |
| author_sort | Nimesh Shukla |
| collection | DOAJ |
| description | Compatible osmolytes are a broad class of small organic molecules employed by living systems to combat environmental stress by enhancing the native protein structure. The molecular features that make for a superior biopreservation remain elusive. Through the use of time-resolved and steady-state spectroscopic techniques, in combination with molecular simulation, insight into what makes one molecule a more effective compatible osmolyte can be gained. Disaccharides differing only in their glycosidic bonds can exhibit different degrees of stabilization against thermal denaturation. The degree to which each sugar is preferentially excluded may explain these differences. The present work examines the biopreservation and hydration of trehalose, maltose, and gentiobiose. |
| first_indexed | 2024-03-10T03:43:03Z |
| format | Article |
| id | doaj.art-761524e9f4f94a3f9afd9b793dce123f |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T03:43:03Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-761524e9f4f94a3f9afd9b793dce123f2023-11-23T09:14:50ZengMDPI AGLife2075-17292021-12-011112139410.3390/life11121394Conformational Consequences for Compatible Osmolytes on Thermal DenaturationNimesh Shukla0Brianna Bembenek1Erika A. Taylor2Christina M. Othon3Department of Physics, Wesleyan University, Middletown, CT 06459, USADepartment of Physics, Ripon College, Oxford OX44 9EX, UKDepartment of Chemistry, Wesleyan University, Middletown, CT 06459, USADepartment of Physics, Ripon College, Oxford OX44 9EX, UKCompatible osmolytes are a broad class of small organic molecules employed by living systems to combat environmental stress by enhancing the native protein structure. The molecular features that make for a superior biopreservation remain elusive. Through the use of time-resolved and steady-state spectroscopic techniques, in combination with molecular simulation, insight into what makes one molecule a more effective compatible osmolyte can be gained. Disaccharides differing only in their glycosidic bonds can exhibit different degrees of stabilization against thermal denaturation. The degree to which each sugar is preferentially excluded may explain these differences. The present work examines the biopreservation and hydration of trehalose, maltose, and gentiobiose.https://www.mdpi.com/2075-1729/11/12/1394hydration dynamicscompatible osmolytetime-dependent Stokes shiftthermal denaturationdisaccharide |
| spellingShingle | Nimesh Shukla Brianna Bembenek Erika A. Taylor Christina M. Othon Conformational Consequences for Compatible Osmolytes on Thermal Denaturation Life hydration dynamics compatible osmolyte time-dependent Stokes shift thermal denaturation disaccharide |
| title | Conformational Consequences for Compatible Osmolytes on Thermal Denaturation |
| title_full | Conformational Consequences for Compatible Osmolytes on Thermal Denaturation |
| title_fullStr | Conformational Consequences for Compatible Osmolytes on Thermal Denaturation |
| title_full_unstemmed | Conformational Consequences for Compatible Osmolytes on Thermal Denaturation |
| title_short | Conformational Consequences for Compatible Osmolytes on Thermal Denaturation |
| title_sort | conformational consequences for compatible osmolytes on thermal denaturation |
| topic | hydration dynamics compatible osmolyte time-dependent Stokes shift thermal denaturation disaccharide |
| url | https://www.mdpi.com/2075-1729/11/12/1394 |
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