Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment
The USDA-FSIS has zero tolerance for <i>E. coli</i> O157:H7 in raw ground beef. Currently, FSIS collects samples from beef processing facilities and ships them overnight to regional testing laboratories. Pathogen detection requires robust methods that employ an initial 15–24 h culture en...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/22/3653 |
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| author | Chuyan Chen Claudia P. Coronel-Aguilera Bruce M. Applegate Andrew G. Gehring Arun K. Bhunia George C. Paoli |
| author_facet | Chuyan Chen Claudia P. Coronel-Aguilera Bruce M. Applegate Andrew G. Gehring Arun K. Bhunia George C. Paoli |
| author_sort | Chuyan Chen |
| collection | DOAJ |
| description | The USDA-FSIS has zero tolerance for <i>E. coli</i> O157:H7 in raw ground beef. Currently, FSIS collects samples from beef processing facilities and ships them overnight to regional testing laboratories. Pathogen detection requires robust methods that employ an initial 15–24 h culture enrichment. This study assessed the potential of using the ΦV10<i>nluc</i> phage-based luminescence detection assay during enrichment while the sample is in transit. Parameters including phage concentrations, temperature, and media-to-sample ratios were evaluated. Results in liquid media showed that 1.73× 10<sup>3</sup> pfu/mL of ΦV10<i>nluc</i> was able to detect 2 CFU in 10 h. The detection of <i>E. coli</i> O157:H7 was further evaluated in kinetic studies using ratios of 1:3, 1:2, and 1:1 ground beef sample to enrichment media, yielding positive results for as little as 2–3 CFU in 325 g ground beef in about 15 h at 37 °C. These results suggest that this approach is feasible, allowing the detection of a presumptive positive upon arrival of the sample to the testing lab. As the current cargo hold controlled temperature is required to be 15–25 °C, the need for elevated temperature should be easily addressed. If successful, this approach could be expanded to other pathogens and foods. |
| first_indexed | 2024-03-09T18:20:39Z |
| format | Article |
| id | doaj.art-76198a010bd1469fb1b63394b245b178 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T18:20:39Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-76198a010bd1469fb1b63394b245b1782023-11-24T08:22:09ZengMDPI AGFoods2304-81582022-11-011122365310.3390/foods11223653Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample ShipmentChuyan Chen0Claudia P. Coronel-Aguilera1Bruce M. Applegate2Andrew G. Gehring3Arun K. Bhunia4George C. Paoli5Department of Food Science, Purdue University, West Lafayette, IN 47907, USADepartment of Food Science, Purdue University, West Lafayette, IN 47907, USADepartment of Food Science, Purdue University, West Lafayette, IN 47907, USAMolecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA 19038, USADepartment of Food Science, Purdue University, West Lafayette, IN 47907, USAMolecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Wyndmoor, PA 19038, USAThe USDA-FSIS has zero tolerance for <i>E. coli</i> O157:H7 in raw ground beef. Currently, FSIS collects samples from beef processing facilities and ships them overnight to regional testing laboratories. Pathogen detection requires robust methods that employ an initial 15–24 h culture enrichment. This study assessed the potential of using the ΦV10<i>nluc</i> phage-based luminescence detection assay during enrichment while the sample is in transit. Parameters including phage concentrations, temperature, and media-to-sample ratios were evaluated. Results in liquid media showed that 1.73× 10<sup>3</sup> pfu/mL of ΦV10<i>nluc</i> was able to detect 2 CFU in 10 h. The detection of <i>E. coli</i> O157:H7 was further evaluated in kinetic studies using ratios of 1:3, 1:2, and 1:1 ground beef sample to enrichment media, yielding positive results for as little as 2–3 CFU in 325 g ground beef in about 15 h at 37 °C. These results suggest that this approach is feasible, allowing the detection of a presumptive positive upon arrival of the sample to the testing lab. As the current cargo hold controlled temperature is required to be 15–25 °C, the need for elevated temperature should be easily addressed. If successful, this approach could be expanded to other pathogens and foods.https://www.mdpi.com/2304-8158/11/22/3653<i>E. coli</i> O157:H7Shiga toxin-producing <i>E. coli</i>bacteriophageculture enrichmentfoodborne pathogenpathogen detection |
| spellingShingle | Chuyan Chen Claudia P. Coronel-Aguilera Bruce M. Applegate Andrew G. Gehring Arun K. Bhunia George C. Paoli Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment Foods <i>E. coli</i> O157:H7 Shiga toxin-producing <i>E. coli</i> bacteriophage culture enrichment foodborne pathogen pathogen detection |
| title | Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment |
| title_full | Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment |
| title_fullStr | Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment |
| title_full_unstemmed | Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment |
| title_short | Studies on Simultaneous Enrichment and Detection of <i>Escherichia coli</i> O157:H7 during Sample Shipment |
| title_sort | studies on simultaneous enrichment and detection of i escherichia coli i o157 h7 during sample shipment |
| topic | <i>E. coli</i> O157:H7 Shiga toxin-producing <i>E. coli</i> bacteriophage culture enrichment foodborne pathogen pathogen detection |
| url | https://www.mdpi.com/2304-8158/11/22/3653 |
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