Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research
Nuclear magnetic resonance (NMR) spectroscopy is one of the principal analytical techniques for metabolomics. It has the advantages of minimal sample preparation and high reproducibility, making it an ideal technique for generating large amounts of metabolomics data for biobanks and large-scale stud...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/12/10/963 |
| _version_ | 1797471428195385344 |
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| author | Katherine Huang Natalie Thomas Paul R. Gooley Christopher W. Armstrong |
| author_facet | Katherine Huang Natalie Thomas Paul R. Gooley Christopher W. Armstrong |
| author_sort | Katherine Huang |
| collection | DOAJ |
| description | Nuclear magnetic resonance (NMR) spectroscopy is one of the principal analytical techniques for metabolomics. It has the advantages of minimal sample preparation and high reproducibility, making it an ideal technique for generating large amounts of metabolomics data for biobanks and large-scale studies. Metabolomics is a popular “omics” technology and has established itself as a comprehensive exploratory biomarker tool; however, it has yet to reach its collaborative potential in data collation due to the lack of standardisation of the metabolomics workflow seen across small-scale studies. This systematic review compiles the different NMR metabolomics methods used for serum, plasma, and urine studies, from sample collection to data analysis, that were most popularly employed over a two-year period in 2019 and 2020. It also outlines how these methods influence the raw data and the downstream interpretations, and the importance of reporting for reproducibility and result validation. This review can act as a valuable summary of NMR metabolomic workflows that are actively used in human biofluid research and will help guide the workflow choice for future research. |
| first_indexed | 2024-03-09T19:49:16Z |
| format | Article |
| id | doaj.art-761d0d5c3f9a4ed6a8d6dbbc27540ac0 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T19:49:16Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-761d0d5c3f9a4ed6a8d6dbbc27540ac02023-11-24T01:15:59ZengMDPI AGMetabolites2218-19892022-10-01121096310.3390/metabo12100963Systematic Review of NMR-Based Metabolomics Practices in Human Disease ResearchKatherine Huang0Natalie Thomas1Paul R. Gooley2Christopher W. Armstrong3Department of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC 3010, AustraliaDepartment of Biochemistry and Pharmacology, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC 3010, AustraliaNuclear magnetic resonance (NMR) spectroscopy is one of the principal analytical techniques for metabolomics. It has the advantages of minimal sample preparation and high reproducibility, making it an ideal technique for generating large amounts of metabolomics data for biobanks and large-scale studies. Metabolomics is a popular “omics” technology and has established itself as a comprehensive exploratory biomarker tool; however, it has yet to reach its collaborative potential in data collation due to the lack of standardisation of the metabolomics workflow seen across small-scale studies. This systematic review compiles the different NMR metabolomics methods used for serum, plasma, and urine studies, from sample collection to data analysis, that were most popularly employed over a two-year period in 2019 and 2020. It also outlines how these methods influence the raw data and the downstream interpretations, and the importance of reporting for reproducibility and result validation. This review can act as a valuable summary of NMR metabolomic workflows that are actively used in human biofluid research and will help guide the workflow choice for future research.https://www.mdpi.com/2218-1989/12/10/963NMR spectroscopymetabolomicsworkflowvariationstandardisation |
| spellingShingle | Katherine Huang Natalie Thomas Paul R. Gooley Christopher W. Armstrong Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research Metabolites NMR spectroscopy metabolomics workflow variation standardisation |
| title | Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research |
| title_full | Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research |
| title_fullStr | Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research |
| title_full_unstemmed | Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research |
| title_short | Systematic Review of NMR-Based Metabolomics Practices in Human Disease Research |
| title_sort | systematic review of nmr based metabolomics practices in human disease research |
| topic | NMR spectroscopy metabolomics workflow variation standardisation |
| url | https://www.mdpi.com/2218-1989/12/10/963 |
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