Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract
The influence of stabilizing activity of citric buffers on betacyanins, as well as their thermal dehydrogenation and decarboxylation in a beetroot betalain-rich extract (BRE), was studied at pH 3–8 and temperature 30, 50 and 85 °C with an additional effect of EDTA. In acetate/phosphate buffers, the...
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/9054 |
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| author | Katarzyna Sutor-Świeży Justyna Proszek Łukasz Popenda Sławomir Wybraniec |
| author_facet | Katarzyna Sutor-Świeży Justyna Proszek Łukasz Popenda Sławomir Wybraniec |
| author_sort | Katarzyna Sutor-Świeży |
| collection | DOAJ |
| description | The influence of stabilizing activity of citric buffers on betacyanins, as well as their thermal dehydrogenation and decarboxylation in a beetroot betalain-rich extract (BRE), was studied at pH 3–8 and temperature 30, 50 and 85 °C with an additional effect of EDTA. In acetate/phosphate buffers, the highest stability is observed at pH 5 and it decreases toward pH 3 as well as pH 8, which is more remarkable at 85 °C. For the citrates, a contradictory effect was observed. Citric buffers tend to stabilize the substrate pigments and their intermediary products in acidic solutions, although increase their reactivity at pH 6–8. The highest impact of EDTA addition on pigment retention in acetate buffers is observed at 85 °C and pH 3–5 as well as 8, reflecting the preserving activity of EDTA at the most unfavorable conditions. At lower temperatures, pigment stability in more acidic conditions is still at higher levels even without addition of citrates or EDTA. The most striking effect on generation of betanin derivatives during heating is 2-decarboxylation which preferentially proceeds in the most acidic environment and this generation rate at 85 °C is much higher in the citrate buffers compared to acetates. |
| first_indexed | 2024-03-09T16:01:21Z |
| format | Article |
| id | doaj.art-761d5b2821d4458196d61a5601910146 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:01:21Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-761d5b2821d4458196d61a56019101462023-11-24T17:02:01ZengMDPI AGMolecules1420-30492022-12-012724905410.3390/molecules27249054Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich ExtractKatarzyna Sutor-Świeży0Justyna Proszek1Łukasz Popenda2Sławomir Wybraniec3Faculty of Chemical Engineering and Technology, Department C-1, Cracow University of Technology, ul. Warszawska 24, 31-155 Cracow, PolandFaculty of Chemical Engineering and Technology, Department C-1, Cracow University of Technology, ul. Warszawska 24, 31-155 Cracow, PolandNanoBioMedical Centre, Adam Mickiewicz University, ul. Wszechnicy Piastowskiej 3, 61-614 Poznan, PolandFaculty of Chemical Engineering and Technology, Department C-1, Cracow University of Technology, ul. Warszawska 24, 31-155 Cracow, PolandThe influence of stabilizing activity of citric buffers on betacyanins, as well as their thermal dehydrogenation and decarboxylation in a beetroot betalain-rich extract (BRE), was studied at pH 3–8 and temperature 30, 50 and 85 °C with an additional effect of EDTA. In acetate/phosphate buffers, the highest stability is observed at pH 5 and it decreases toward pH 3 as well as pH 8, which is more remarkable at 85 °C. For the citrates, a contradictory effect was observed. Citric buffers tend to stabilize the substrate pigments and their intermediary products in acidic solutions, although increase their reactivity at pH 6–8. The highest impact of EDTA addition on pigment retention in acetate buffers is observed at 85 °C and pH 3–5 as well as 8, reflecting the preserving activity of EDTA at the most unfavorable conditions. At lower temperatures, pigment stability in more acidic conditions is still at higher levels even without addition of citrates or EDTA. The most striking effect on generation of betanin derivatives during heating is 2-decarboxylation which preferentially proceeds in the most acidic environment and this generation rate at 85 °C is much higher in the citrate buffers compared to acetates.https://www.mdpi.com/1420-3049/27/24/9054dehydrogenationdecarboxylationxanbetaninneobetaninred beet rootcolorants |
| spellingShingle | Katarzyna Sutor-Świeży Justyna Proszek Łukasz Popenda Sławomir Wybraniec Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract Molecules dehydrogenation decarboxylation xanbetanin neobetanin red beet root colorants |
| title | Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract |
| title_full | Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract |
| title_fullStr | Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract |
| title_full_unstemmed | Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract |
| title_short | Influence of Citrates and EDTA on Oxidation and Decarboxylation of Betacyanins in Red Beet (<i>Beta vulgaris</i> L.) Betalain-Rich Extract |
| title_sort | influence of citrates and edta on oxidation and decarboxylation of betacyanins in red beet i beta vulgaris i l betalain rich extract |
| topic | dehydrogenation decarboxylation xanbetanin neobetanin red beet root colorants |
| url | https://www.mdpi.com/1420-3049/27/24/9054 |
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