PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography
The oceans represent 97% of all water on Earth and contain microscopic, drifting life, plankton, which drives global biogeochemical cycles. A major hurdle in assessing marine plankton is the planetary scale of the oceans and the logistical and economic constraints associated with their sampling. Thi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.949428/full |
| _version_ | 1811287812005167104 |
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| author | Thibaut Pollina Thibaut Pollina Adam G. Larson Adam G. Larson Fabien Lombard Fabien Lombard Fabien Lombard Fabien Lombard Hongquan Li David Le Guen Sébastien Colin Sébastien Colin Colomban de Vargas Colomban de Vargas Colomban de Vargas Manu Prakash Manu Prakash |
| author_facet | Thibaut Pollina Thibaut Pollina Adam G. Larson Adam G. Larson Fabien Lombard Fabien Lombard Fabien Lombard Fabien Lombard Hongquan Li David Le Guen Sébastien Colin Sébastien Colin Colomban de Vargas Colomban de Vargas Colomban de Vargas Manu Prakash Manu Prakash |
| author_sort | Thibaut Pollina |
| collection | DOAJ |
| description | The oceans represent 97% of all water on Earth and contain microscopic, drifting life, plankton, which drives global biogeochemical cycles. A major hurdle in assessing marine plankton is the planetary scale of the oceans and the logistical and economic constraints associated with their sampling. This difficulty is reflected in the limited amount of scientifically equipped fleets and affordable equipment. Here we present a modular hardware/software open-source strategy for building a versatile, re-configurable imaging platform - the PlanktoScope - that can be adapted to a number of applications in aquatic biology and ecology. We demonstrate high-throughput quantitative imaging of laboratory and field plankton samples while enabling rapid device reconfiguration to match the evolving needs of the sampler. The presented versions of PlanktoScope are capable of autonomously imaging 1.7 ml per minute with a 2.8 µm/px resolution and can be controlled from any WiFi-enabled device. The PlanktoScope’s small size, ease of use, and low cost - under $1000 in parts - enable its deployment for customizable monitoring of laboratory cultures or natural micro-plankton communities. This also paves the way toward consistent and long-term measurement of plankton diversity by an international fleet of citizen vessels at the planetary scale. |
| first_indexed | 2024-04-13T03:26:18Z |
| format | Article |
| id | doaj.art-761db34337a04a9c93d662d534c49192 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-13T03:26:18Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-761db34337a04a9c93d662d534c491922022-12-22T03:04:39ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-07-01910.3389/fmars.2022.949428949428PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen OceanographyThibaut Pollina0Thibaut Pollina1Adam G. Larson2Adam G. Larson3Fabien Lombard4Fabien Lombard5Fabien Lombard6Fabien Lombard7Hongquan Li8David Le Guen9Sébastien Colin10Sébastien Colin11Colomban de Vargas12Colomban de Vargas13Colomban de Vargas14Manu Prakash15Manu Prakash16Bioengineering, Stanford University, Stanford, CA, United StatesPlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FranceBioengineering, Stanford University, Stanford, CA, United StatesPlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FrancePlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FranceResearch Federation for the Study of Global Ocean Systems Ecology and Evolution, FR2022/Tara Global Ocean Systems Ecology & Evolution (GOSEE), Paris, FranceSorbonne Université, Centre National de la Recherche Scientifique (CNRS), Laboratoire d’Océanographie de Villefranche, Villefranche-sur-mer, FranceBiology Institut Universitaire de France (IUF), Paris, FranceBioengineering, Stanford University, Stanford, CA, United StatesPlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FrancePlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FranceMax Planck Institute for Biology Tübingen, BioOptics Facility, Max-Planck-Ring 5, Tübingen, GermanyPlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FranceResearch Federation for the Study of Global Ocean Systems Ecology and Evolution, FR2022/Tara Global Ocean Systems Ecology & Evolution (GOSEE), Paris, FranceCNRS, Sorbonne Université, Station Biologique de Roscoff, UMR7144, ECOMAP - Ecology of Marine Plankton, Roscoff, FranceBioengineering, Stanford University, Stanford, CA, United StatesPlankton Planet Non-Governmental Organization (NGO), Station Biologique de Roscoff, Roscoff, FranceThe oceans represent 97% of all water on Earth and contain microscopic, drifting life, plankton, which drives global biogeochemical cycles. A major hurdle in assessing marine plankton is the planetary scale of the oceans and the logistical and economic constraints associated with their sampling. This difficulty is reflected in the limited amount of scientifically equipped fleets and affordable equipment. Here we present a modular hardware/software open-source strategy for building a versatile, re-configurable imaging platform - the PlanktoScope - that can be adapted to a number of applications in aquatic biology and ecology. We demonstrate high-throughput quantitative imaging of laboratory and field plankton samples while enabling rapid device reconfiguration to match the evolving needs of the sampler. The presented versions of PlanktoScope are capable of autonomously imaging 1.7 ml per minute with a 2.8 µm/px resolution and can be controlled from any WiFi-enabled device. The PlanktoScope’s small size, ease of use, and low cost - under $1000 in parts - enable its deployment for customizable monitoring of laboratory cultures or natural micro-plankton communities. This also paves the way toward consistent and long-term measurement of plankton diversity by an international fleet of citizen vessels at the planetary scale.https://www.frontiersin.org/articles/10.3389/fmars.2022.949428/fullPlanktoScopemicroplanktonfrugal microscopyquantitative imagingopen source modularity |
| spellingShingle | Thibaut Pollina Thibaut Pollina Adam G. Larson Adam G. Larson Fabien Lombard Fabien Lombard Fabien Lombard Fabien Lombard Hongquan Li David Le Guen Sébastien Colin Sébastien Colin Colomban de Vargas Colomban de Vargas Colomban de Vargas Manu Prakash Manu Prakash PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography Frontiers in Marine Science PlanktoScope microplankton frugal microscopy quantitative imaging open source modularity |
| title | PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography |
| title_full | PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography |
| title_fullStr | PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography |
| title_full_unstemmed | PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography |
| title_short | PlanktoScope: Affordable Modular Quantitative Imaging Platform for Citizen Oceanography |
| title_sort | planktoscope affordable modular quantitative imaging platform for citizen oceanography |
| topic | PlanktoScope microplankton frugal microscopy quantitative imaging open source modularity |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.949428/full |
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