Unique cellular immune signatures of multisystem inflammatory syndrome in children
The clinical presentation of MIS-C overlaps with other infectious/non-infectious diseases such as acute COVID-19, Kawasaki disease, acute dengue, enteric fever, and systemic lupus erythematosus. We examined the ex-vivo cellular parameters with the aim of distinguishing MIS-C from other syndromes wit...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-11-01
|
| Series: | PLoS Pathogens |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629618/?tool=EBI |
| _version_ | 1798044719012380672 |
|---|---|
| author | Anuradha Rajamanickam Pavan Kumar Nathella Aishwarya Venkataraman Poovazhagi Varadarjan Srinithi Kannan Arul Nancy Pandiarajan Rachel Mariam Renji Elayarani Elavarasan Akshith Thimmaiah Kandasamy Sasidaran Nedunchelian Krishnamoorthy Suresh Natarajan Ganesh Ramaswamy Balasubramanian Sundaram Sulochana Putlibai Syed Hissar Elilarasi Selladurai K. Ranganathan Uma Devi Thomas B. Nutman Subash Babu |
| author_facet | Anuradha Rajamanickam Pavan Kumar Nathella Aishwarya Venkataraman Poovazhagi Varadarjan Srinithi Kannan Arul Nancy Pandiarajan Rachel Mariam Renji Elayarani Elavarasan Akshith Thimmaiah Kandasamy Sasidaran Nedunchelian Krishnamoorthy Suresh Natarajan Ganesh Ramaswamy Balasubramanian Sundaram Sulochana Putlibai Syed Hissar Elilarasi Selladurai K. Ranganathan Uma Devi Thomas B. Nutman Subash Babu |
| author_sort | Anuradha Rajamanickam |
| collection | DOAJ |
| description | The clinical presentation of MIS-C overlaps with other infectious/non-infectious diseases such as acute COVID-19, Kawasaki disease, acute dengue, enteric fever, and systemic lupus erythematosus. We examined the ex-vivo cellular parameters with the aim of distinguishing MIS-C from other syndromes with overlapping clinical presentations. MIS-C children differed from children with non-MIS-C conditions by having increased numbers of naïve CD8+ T cells, naïve, immature and atypical memory B cells and diminished numbers of transitional memory, stem cell memory, central and effector memory CD4+ and CD8+ T cells, classical, activated memory B and plasma cells and monocyte (intermediate and non-classical) and dendritic cell (plasmacytoid and myeloid) subsets. All of the above alterations were significantly reversed at 6–9 months post-recovery in MIS-C. Thus, MIS-C is characterized by a distinct cellular signature that distinguishes it from other syndromes with overlapping clinical presentations. Trial Registration: ClinicalTrials.gov clinicaltrial.gov. No: NCT04844242. Author summary Multisystem inflammatory syndrome in children (MIS-C) is a severe disease that happens several weeks after severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. We performed an in-depth analysis of immune cell subsets and demonstrated that children with MIS-C had a distinctive cellular signature that differentiates it from other overlapping clinical presentations. T, B and myeloid cellular compartments were altered in MIS-C children. Post-recovery (6–9 months post-treatment), most of these alterations were significantly reversed and returned to normal levels. Thus, MIS-C varies from acute COVID-19, other infectious, non-infectious diseases and healthy pediatric controls by exhibiting a unique cellular signature. |
| first_indexed | 2024-04-11T23:08:46Z |
| format | Article |
| id | doaj.art-76256bbfb0c94d1fb1bdb6b1f20e7838 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-11T23:08:46Z |
| publishDate | 2022-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-76256bbfb0c94d1fb1bdb6b1f20e78382022-12-22T03:57:55ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-11-011811Unique cellular immune signatures of multisystem inflammatory syndrome in childrenAnuradha RajamanickamPavan Kumar NathellaAishwarya VenkataramanPoovazhagi VaradarjanSrinithi KannanArul Nancy PandiarajanRachel Mariam RenjiElayarani ElavarasanAkshith ThimmaiahKandasamy SasidaranNedunchelian KrishnamoorthySuresh NatarajanGanesh RamaswamyBalasubramanian SundaramSulochana PutlibaiSyed HissarElilarasi SelladuraiK. Ranganathan Uma DeviThomas B. NutmanSubash BabuThe clinical presentation of MIS-C overlaps with other infectious/non-infectious diseases such as acute COVID-19, Kawasaki disease, acute dengue, enteric fever, and systemic lupus erythematosus. We examined the ex-vivo cellular parameters with the aim of distinguishing MIS-C from other syndromes with overlapping clinical presentations. MIS-C children differed from children with non-MIS-C conditions by having increased numbers of naïve CD8+ T cells, naïve, immature and atypical memory B cells and diminished numbers of transitional memory, stem cell memory, central and effector memory CD4+ and CD8+ T cells, classical, activated memory B and plasma cells and monocyte (intermediate and non-classical) and dendritic cell (plasmacytoid and myeloid) subsets. All of the above alterations were significantly reversed at 6–9 months post-recovery in MIS-C. Thus, MIS-C is characterized by a distinct cellular signature that distinguishes it from other syndromes with overlapping clinical presentations. Trial Registration: ClinicalTrials.gov clinicaltrial.gov. No: NCT04844242. Author summary Multisystem inflammatory syndrome in children (MIS-C) is a severe disease that happens several weeks after severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. We performed an in-depth analysis of immune cell subsets and demonstrated that children with MIS-C had a distinctive cellular signature that differentiates it from other overlapping clinical presentations. T, B and myeloid cellular compartments were altered in MIS-C children. Post-recovery (6–9 months post-treatment), most of these alterations were significantly reversed and returned to normal levels. Thus, MIS-C varies from acute COVID-19, other infectious, non-infectious diseases and healthy pediatric controls by exhibiting a unique cellular signature.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629618/?tool=EBI |
| spellingShingle | Anuradha Rajamanickam Pavan Kumar Nathella Aishwarya Venkataraman Poovazhagi Varadarjan Srinithi Kannan Arul Nancy Pandiarajan Rachel Mariam Renji Elayarani Elavarasan Akshith Thimmaiah Kandasamy Sasidaran Nedunchelian Krishnamoorthy Suresh Natarajan Ganesh Ramaswamy Balasubramanian Sundaram Sulochana Putlibai Syed Hissar Elilarasi Selladurai K. Ranganathan Uma Devi Thomas B. Nutman Subash Babu Unique cellular immune signatures of multisystem inflammatory syndrome in children PLoS Pathogens |
| title | Unique cellular immune signatures of multisystem inflammatory syndrome in children |
| title_full | Unique cellular immune signatures of multisystem inflammatory syndrome in children |
| title_fullStr | Unique cellular immune signatures of multisystem inflammatory syndrome in children |
| title_full_unstemmed | Unique cellular immune signatures of multisystem inflammatory syndrome in children |
| title_short | Unique cellular immune signatures of multisystem inflammatory syndrome in children |
| title_sort | unique cellular immune signatures of multisystem inflammatory syndrome in children |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629618/?tool=EBI |
| work_keys_str_mv | AT anuradharajamanickam uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT pavankumarnathella uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT aishwaryavenkataraman uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT poovazhagivaradarjan uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT srinithikannan uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT arulnancypandiarajan uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT rachelmariamrenji uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT elayaranielavarasan uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT akshiththimmaiah uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT kandasamysasidaran uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT neduncheliankrishnamoorthy uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT sureshnatarajan uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT ganeshramaswamy uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT balasubramaniansundaram uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT sulochanaputlibai uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT syedhissar uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT elilarasiselladurai uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT kranganathanumadevi uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT thomasbnutman uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren AT subashbabu uniquecellularimmunesignaturesofmultisysteminflammatorysyndromeinchildren |