Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient
AbstractCalciphylaxis is a rare cutaneous vascular disease that manifests with intolerable pains, non-healing skin wounds, histologically characterized by calcification, fibrointimal hyperplasia, and microvessel thrombosis. Currently, there are no standardized guidelines for this disease. Recent stu...
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2023.2218483 |
| _version_ | 1797658513598578688 |
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| author | Anning Bian Xiaoxue Ye Jing Wang Ming Zeng Jiayin Liu Kang Liu Song Ning Yugui Cui Shaowen Tang Xueqiang Xu Yanggang Yuan Zhonglan Su Yan Lu Jing Zhou Xiang Ma Guang Yang Yaoyu Huang Feng Chen Youjia Yu Mufeng Gu Xiaolin Lv Ling Wang Jing Zhao Xiuqin Wang Ningxia Liang Changying Xing Lianju Qin Ningning Wang |
| author_facet | Anning Bian Xiaoxue Ye Jing Wang Ming Zeng Jiayin Liu Kang Liu Song Ning Yugui Cui Shaowen Tang Xueqiang Xu Yanggang Yuan Zhonglan Su Yan Lu Jing Zhou Xiang Ma Guang Yang Yaoyu Huang Feng Chen Youjia Yu Mufeng Gu Xiaolin Lv Ling Wang Jing Zhao Xiuqin Wang Ningxia Liang Changying Xing Lianju Qin Ningning Wang |
| author_sort | Anning Bian |
| collection | DOAJ |
| description | AbstractCalciphylaxis is a rare cutaneous vascular disease that manifests with intolerable pains, non-healing skin wounds, histologically characterized by calcification, fibrointimal hyperplasia, and microvessel thrombosis. Currently, there are no standardized guidelines for this disease. Recent studies have recognized a high prevalence of thrombophilias and hypercoagulable conditions in calciphylaxis patients. Here, we report a case of uremic calciphylaxis patient whom was refractory to conventional treatments and then received a salvage strategy with intravenous and local hAMSC application. In order to investigate the therapeutic mechanism of hAMSCs from the novel perspective of hypercoagulability, coagulation-related indicators, wound status, quality of life and skin biopsy were followed up. Polymerase chain reaction (PCR) was performed to determine the distribution of hAMSCs in multiple tissues including lung, kidney and muscle after infusion of hAMSCs for 24 h, 1 week and 1 month in mice aiming to investigate whether hAMSCs retain locally active roles after intravenous administration. Improvement of hypercoagulable condition involving correction of platelet, D-dimer and plasminogen levels, skin regeneration and pain alleviation were revealed after hAMSC administration over one-year period. Skin biopsy pathology suggested regenerative tissues after 1 month hAMSC application and full epidermal regeneration after 20 months hAMSC treatment. PCR analysis indicated that hAMSCs were homing in lung, kidney and muscle tissues of mice even until tail vein injection of hAMSCs for 1 month. We propose that hypercoagulability is a promising therapeutic target of calciphylaxis patients, which can be effectively improved by hAMSC treatment. |
| first_indexed | 2024-03-11T18:00:05Z |
| format | Article |
| id | doaj.art-7629f7b09b7d49f18ee083deb0602a5d |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-03-11T18:00:05Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-7629f7b09b7d49f18ee083deb0602a5d2023-10-17T09:23:23ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492023-12-0145110.1080/0886022X.2023.2218483Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patientAnning Bian0Xiaoxue Ye1Jing Wang2Ming Zeng3Jiayin Liu4Kang Liu5Song Ning6Yugui Cui7Shaowen Tang8Xueqiang Xu9Yanggang Yuan10Zhonglan Su11Yan Lu12Jing Zhou13Xiang Ma14Guang Yang15Yaoyu Huang16Feng Chen17Youjia Yu18Mufeng Gu19Xiaolin Lv20Ling Wang21Jing Zhao22Xiuqin Wang23Ningxia Liang24Changying Xing25Lianju Qin26Ningning Wang27Department of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaState Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, Center of Stem Cell Research and Clinical Practice, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaState Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, Center of Stem Cell Research and Clinical Practice, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaState Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, Center of Stem Cell Research and Clinical Practice, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Jiangning District, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Dermatology, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of ChinaDepartment of Dermatology, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of ChinaState Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, Center of Stem Cell Research and Clinical Practice, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaState Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, Center of Stem Cell Research and Clinical Practice, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Forensic Medicine, School of Basic Medical Sciences, Nanjing Medical University, Jiangning District, Nanjing, People’s Republic of ChinaDepartment of Forensic Medicine, School of Basic Medical Sciences, Nanjing Medical University, Jiangning District, Nanjing, People’s Republic of ChinaDepartment of Human Anatomy, School of Basic Medical Sciences, Nanjing Medical University, Jiangning District, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Outpatient Treatment Clinic, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of International Cooperation, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaAcademy of Clinical and Translational Research, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaState Key Laboratory of Reproductive Medicine, Center of Clinical Reproductive Medicine, Center of Stem Cell Research and Clinical Practice, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaDepartment of Nephrology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, People’s Republic of ChinaAbstractCalciphylaxis is a rare cutaneous vascular disease that manifests with intolerable pains, non-healing skin wounds, histologically characterized by calcification, fibrointimal hyperplasia, and microvessel thrombosis. Currently, there are no standardized guidelines for this disease. Recent studies have recognized a high prevalence of thrombophilias and hypercoagulable conditions in calciphylaxis patients. Here, we report a case of uremic calciphylaxis patient whom was refractory to conventional treatments and then received a salvage strategy with intravenous and local hAMSC application. In order to investigate the therapeutic mechanism of hAMSCs from the novel perspective of hypercoagulability, coagulation-related indicators, wound status, quality of life and skin biopsy were followed up. Polymerase chain reaction (PCR) was performed to determine the distribution of hAMSCs in multiple tissues including lung, kidney and muscle after infusion of hAMSCs for 24 h, 1 week and 1 month in mice aiming to investigate whether hAMSCs retain locally active roles after intravenous administration. Improvement of hypercoagulable condition involving correction of platelet, D-dimer and plasminogen levels, skin regeneration and pain alleviation were revealed after hAMSC administration over one-year period. Skin biopsy pathology suggested regenerative tissues after 1 month hAMSC application and full epidermal regeneration after 20 months hAMSC treatment. PCR analysis indicated that hAMSCs were homing in lung, kidney and muscle tissues of mice even until tail vein injection of hAMSCs for 1 month. We propose that hypercoagulability is a promising therapeutic target of calciphylaxis patients, which can be effectively improved by hAMSC treatment.https://www.tandfonline.com/doi/10.1080/0886022X.2023.2218483Calciphylaxischronic kidney diseasehypercoagulabilitythrombophiliashuman amnion-derived mesenchymal stem cell |
| spellingShingle | Anning Bian Xiaoxue Ye Jing Wang Ming Zeng Jiayin Liu Kang Liu Song Ning Yugui Cui Shaowen Tang Xueqiang Xu Yanggang Yuan Zhonglan Su Yan Lu Jing Zhou Xiang Ma Guang Yang Yaoyu Huang Feng Chen Youjia Yu Mufeng Gu Xiaolin Lv Ling Wang Jing Zhao Xiuqin Wang Ningxia Liang Changying Xing Lianju Qin Ningning Wang Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient Renal Failure Calciphylaxis chronic kidney disease hypercoagulability thrombophilias human amnion-derived mesenchymal stem cell |
| title | Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient |
| title_full | Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient |
| title_fullStr | Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient |
| title_full_unstemmed | Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient |
| title_short | Therapeutic effects and mechanism of human amnion-derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient |
| title_sort | therapeutic effects and mechanism of human amnion derived mesenchymal stem cells on hypercoagulability in a uremic calciphylaxis patient |
| topic | Calciphylaxis chronic kidney disease hypercoagulability thrombophilias human amnion-derived mesenchymal stem cell |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2023.2218483 |
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