A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea
Abstract Synthetic fungicides are eco-unfriendly to agriculture and the environment. Agricultural Jiaosu (AJ), which originates from organic wastes, has the potential to be a substitute for synthetic fungicides. In this study, the characteristics of AJ and its antifungal activity against Botrytis ci...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-12-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-020-01156-7 |
| _version_ | 1819295736340152320 |
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| author | Yue Zhang Youhui Gao Zehui Zheng Xingyao Meng Yafan Cai Jianbin Liu Yuegao Hu Shuangdui Yan Xiaofen Wang |
| author_facet | Yue Zhang Youhui Gao Zehui Zheng Xingyao Meng Yafan Cai Jianbin Liu Yuegao Hu Shuangdui Yan Xiaofen Wang |
| author_sort | Yue Zhang |
| collection | DOAJ |
| description | Abstract Synthetic fungicides are eco-unfriendly to agriculture and the environment. Agricultural Jiaosu (AJ), which originates from organic wastes, has the potential to be a substitute for synthetic fungicides. In this study, the characteristics of AJ and its antifungal activity against Botrytis cinerea were investigated for the first time. AJ was rich in lactic acid (4.46 g/L), acetic acid (1.52 g/L), Lactobacillus (72.45%) and Acetobacter (15.23%), which was a microbial ecosystem consisting of acid-based substances (AS) and beneficial microorganisms (BM). The results of the antifungal assays suggested that B. cinerea was effectively inhibited by AJ, with the half-maximal inhibitory concentration (IC50) of 9.24%. AJ showed the strongest and most-lasting inhibitory effect compared to cell-free supernatant and microbial solution of AJ, indicating that AS and BM and their synergistic effect contributed to the antifungal activity of AJ. Two-step inhibition’ is an antifungal mode of AJ. Firstly, AS not only inhibited the pathogen directly but also provided a dominant niche for BM of AJ. Then, BM in AJ, especially Acetobacter, proliferated and metabolized acetic acid continuously. Thus, AJ achieved high-efficiency and long-acting inhibition. AJ is a promising biological agent considering its features of an eco-friendly, low-cost and easy-to-operate biological agent in rural areas. |
| first_indexed | 2024-12-24T04:46:57Z |
| format | Article |
| id | doaj.art-762b25615f3742fbb591db282375b448 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-24T04:46:57Z |
| publishDate | 2020-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-762b25615f3742fbb591db282375b4482022-12-21T17:14:39ZengSpringerOpenAMB Express2191-08552020-12-0110111110.1186/s13568-020-01156-7A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinereaYue Zhang0Youhui Gao1Zehui Zheng2Xingyao Meng3Yafan Cai4Jianbin Liu5Yuegao Hu6Shuangdui Yan7Xiaofen Wang8College of Agronomy and Biotechnology, China Agricultural UniversityCollege of Agronomy and Biotechnology, China Agricultural UniversityCollege of Agronomy and Biotechnology, China Agricultural UniversityBeijing Technology and Business UniversityDepartment of Biochemical Conversion, Deutsches Biomassforschungszentrum gemeinnütziges GmbHInstitute of Plant Nutrition and Resources, Beijing Academy of Agricultural and Forestry SciencesCollege of Agronomy and Biotechnology, China Agricultural UniversityCollege of Resource and Environmental Science, Shanxi Agricultural UniversityCollege of Agronomy and Biotechnology, China Agricultural UniversityAbstract Synthetic fungicides are eco-unfriendly to agriculture and the environment. Agricultural Jiaosu (AJ), which originates from organic wastes, has the potential to be a substitute for synthetic fungicides. In this study, the characteristics of AJ and its antifungal activity against Botrytis cinerea were investigated for the first time. AJ was rich in lactic acid (4.46 g/L), acetic acid (1.52 g/L), Lactobacillus (72.45%) and Acetobacter (15.23%), which was a microbial ecosystem consisting of acid-based substances (AS) and beneficial microorganisms (BM). The results of the antifungal assays suggested that B. cinerea was effectively inhibited by AJ, with the half-maximal inhibitory concentration (IC50) of 9.24%. AJ showed the strongest and most-lasting inhibitory effect compared to cell-free supernatant and microbial solution of AJ, indicating that AS and BM and their synergistic effect contributed to the antifungal activity of AJ. Two-step inhibition’ is an antifungal mode of AJ. Firstly, AS not only inhibited the pathogen directly but also provided a dominant niche for BM of AJ. Then, BM in AJ, especially Acetobacter, proliferated and metabolized acetic acid continuously. Thus, AJ achieved high-efficiency and long-acting inhibition. AJ is a promising biological agent considering its features of an eco-friendly, low-cost and easy-to-operate biological agent in rural areas.https://doi.org/10.1186/s13568-020-01156-7Agricultural JiaosuAntifungal activityBiological agentSynergistic effectsBotrytis cinerea |
| spellingShingle | Yue Zhang Youhui Gao Zehui Zheng Xingyao Meng Yafan Cai Jianbin Liu Yuegao Hu Shuangdui Yan Xiaofen Wang A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea AMB Express Agricultural Jiaosu Antifungal activity Biological agent Synergistic effects Botrytis cinerea |
| title | A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea |
| title_full | A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea |
| title_fullStr | A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea |
| title_full_unstemmed | A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea |
| title_short | A microbial ecosystem: agricultural Jiaosu achieves effective and lasting antifungal activity against Botrytis cinerea |
| title_sort | microbial ecosystem agricultural jiaosu achieves effective and lasting antifungal activity against botrytis cinerea |
| topic | Agricultural Jiaosu Antifungal activity Biological agent Synergistic effects Botrytis cinerea |
| url | https://doi.org/10.1186/s13568-020-01156-7 |
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