Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP
Background: The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran .Methods: Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2011-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | http://journals.tums.ac.ir/upload_files/pdf/17732.pdf |
| _version_ | 1818431740466692096 |
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| author | K Manouchehri Naeini M Asadi M Hashemzade Chaleshtori |
| author_facet | K Manouchehri Naeini M Asadi M Hashemzade Chaleshtori |
| author_sort | K Manouchehri Naeini |
| collection | DOAJ |
| description | Background: The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran .Methods: Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique.Results: Out of thirty samples examined, 6 (20%) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, followed by C. hominis and C. canis , respectively. In this area, the higher prevalence of C. parvum compared with other genotypes is consistent with the distribution of cattle.Conclusion: Farm animals, particularly cattle are the main source of cryptosporidial contamination for recreational waters in this area. |
| first_indexed | 2024-12-14T15:54:07Z |
| format | Article |
| id | doaj.art-762c164c693e4bfb99c61ede36ab9630 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-14T15:54:07Z |
| publishDate | 2011-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-762c164c693e4bfb99c61ede36ab96302022-12-21T22:55:18ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2011-03-01612027Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLPK Manouchehri NaeiniM AsadiM Hashemzade ChaleshtoriBackground: The aim of this study was to detect and characterize Cryptosporidium spp. in water samples collected from recreational ponds of Chaharmahal va Bakhtiyari Province of Iran .Methods: Thirty water samples were collected from November 2009 to May 2010. Each sample contained 10 liters of water. We used the SSU rRNA-based PCR-RFLP technique.Results: Out of thirty samples examined, 6 (20%) were positive for different Cryptosporidium spp. Restriction pattern analysis showed that C. parvum has been the most prevalent genotype, followed by C. hominis and C. canis , respectively. In this area, the higher prevalence of C. parvum compared with other genotypes is consistent with the distribution of cattle.Conclusion: Farm animals, particularly cattle are the main source of cryptosporidial contamination for recreational waters in this area.http://journals.tums.ac.ir/upload_files/pdf/17732.pdfRecreational WatersCryptosporidiumGenotypingNested-PCR-RFLPIran |
| spellingShingle | K Manouchehri Naeini M Asadi M Hashemzade Chaleshtori Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP Iranian Journal of Parasitology Recreational Waters Cryptosporidium Genotyping Nested-PCR-RFLP Iran |
| title | Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP |
| title_full | Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP |
| title_fullStr | Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP |
| title_full_unstemmed | Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP |
| title_short | Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP |
| title_sort | detection and molecular characterization of cryptosporidium species in recreational waters of chaharmahal va bakhtiyari province of iran using nested pcr rflp |
| topic | Recreational Waters Cryptosporidium Genotyping Nested-PCR-RFLP Iran |
| url | http://journals.tums.ac.ir/upload_files/pdf/17732.pdf |
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