Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte
Abstract Background Zygophyllum xanthoxylum is a succulent xerophyte with remarkable tolerance to diverse abiotic stresses. Previous studies have revealed important physiological mechanisms and identified functional genes associated with stress tolerance. However, knowledge of the regulatory genes c...
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1686-1 |
| _version_ | 1811269514458824704 |
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| author | Hongju Yin Mengzhan Li Dingding Li Sardar-Ali Khan Shelley R. Hepworth Suo-Min Wang |
| author_facet | Hongju Yin Mengzhan Li Dingding Li Sardar-Ali Khan Shelley R. Hepworth Suo-Min Wang |
| author_sort | Hongju Yin |
| collection | DOAJ |
| description | Abstract Background Zygophyllum xanthoxylum is a succulent xerophyte with remarkable tolerance to diverse abiotic stresses. Previous studies have revealed important physiological mechanisms and identified functional genes associated with stress tolerance. However, knowledge of the regulatory genes conferring stress tolerance in this species is poorly understood. Results Here, we present a comprehensive analysis of regulatory genes based on the transcriptome of Z. xanthoxylum roots exposed to osmotic stress and salt treatments. Significant changes were observed in transcripts related to known and obscure stress-related hormone signaling pathways, in particular abscisic acid and auxin. Significant changes were also found among key classes of early response regulatory genes encoding protein kinases, transcription factors, and ubiquitin-mediated proteolysis machinery. Network analysis shows a highly integrated matrix formed by these conserved and novel gene products associated with osmotic stress and salt in Z. xanthoxylum. Among them, two previously uncharacterized NAC (NAM/ATAF/CUC) transcription factor genes, ZxNAC083 (Unigene16368_All) and ZxNAC035 (CL6534.Contig1_All), conferred tolerance to salt and drought stress when constitutively overexpressed in Arabidopsis plants. Conclusions This study provides a unique framework for understanding osmotic stress and salt adaptation in Z. xanthoxylum including novel gene targets for engineering stress tolerance in susceptible crop species. |
| first_indexed | 2024-04-12T21:43:25Z |
| format | Article |
| id | doaj.art-762ed3a90f5d4a33a519ad7745558035 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T21:43:25Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-762ed3a90f5d4a33a519ad77455580352022-12-22T03:15:42ZengBMCBMC Plant Biology1471-22292019-02-0119111510.1186/s12870-019-1686-1Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyteHongju Yin0Mengzhan Li1Dingding Li2Sardar-Ali Khan3Shelley R. Hepworth4Suo-Min Wang5State Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou UniversityState Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou UniversityState Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou UniversityState Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou UniversityState Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou UniversityState Key Laboratory of Grassland Agro-ecosystems; Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; College of Pastoral Agriculture Science and Technology, Lanzhou UniversityAbstract Background Zygophyllum xanthoxylum is a succulent xerophyte with remarkable tolerance to diverse abiotic stresses. Previous studies have revealed important physiological mechanisms and identified functional genes associated with stress tolerance. However, knowledge of the regulatory genes conferring stress tolerance in this species is poorly understood. Results Here, we present a comprehensive analysis of regulatory genes based on the transcriptome of Z. xanthoxylum roots exposed to osmotic stress and salt treatments. Significant changes were observed in transcripts related to known and obscure stress-related hormone signaling pathways, in particular abscisic acid and auxin. Significant changes were also found among key classes of early response regulatory genes encoding protein kinases, transcription factors, and ubiquitin-mediated proteolysis machinery. Network analysis shows a highly integrated matrix formed by these conserved and novel gene products associated with osmotic stress and salt in Z. xanthoxylum. Among them, two previously uncharacterized NAC (NAM/ATAF/CUC) transcription factor genes, ZxNAC083 (Unigene16368_All) and ZxNAC035 (CL6534.Contig1_All), conferred tolerance to salt and drought stress when constitutively overexpressed in Arabidopsis plants. Conclusions This study provides a unique framework for understanding osmotic stress and salt adaptation in Z. xanthoxylum including novel gene targets for engineering stress tolerance in susceptible crop species.http://link.springer.com/article/10.1186/s12870-019-1686-1RNA-sequencingOsmotic stressSaltProtein kinasesTranscription factorsProteolysis |
| spellingShingle | Hongju Yin Mengzhan Li Dingding Li Sardar-Ali Khan Shelley R. Hepworth Suo-Min Wang Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte BMC Plant Biology RNA-sequencing Osmotic stress Salt Protein kinases Transcription factors Proteolysis |
| title | Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte |
| title_full | Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte |
| title_fullStr | Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte |
| title_full_unstemmed | Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte |
| title_short | Transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte |
| title_sort | transcriptome analysis reveals regulatory framework for salt and osmotic tolerance in a succulent xerophyte |
| topic | RNA-sequencing Osmotic stress Salt Protein kinases Transcription factors Proteolysis |
| url | http://link.springer.com/article/10.1186/s12870-019-1686-1 |
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