Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility
The aim of this work was to produce hydroxyapatite (HA) granules containing 0, 0.5, 1 and 5 mol. (%) of strontium (Sr), evaluate the physico-chemical properties and also the cytotoxicity by three different parameters of cell viability (ISO 10993-5, 10993-12). The physico-chemical characterization wa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)
2011-12-01
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| Series: | Materials Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392011000400005 |
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| author | Débora dos Santos Tavares Cristiane Xavier Resende Maíra Paiva Quitan Letícia de Oliveira Castro José Mauro Granjeiro Gloria de Almeida Soares |
| author_facet | Débora dos Santos Tavares Cristiane Xavier Resende Maíra Paiva Quitan Letícia de Oliveira Castro José Mauro Granjeiro Gloria de Almeida Soares |
| author_sort | Débora dos Santos Tavares |
| collection | DOAJ |
| description | The aim of this work was to produce hydroxyapatite (HA) granules containing 0, 0.5, 1 and 5 mol. (%) of strontium (Sr), evaluate the physico-chemical properties and also the cytotoxicity by three different parameters of cell viability (ISO 10993-5, 10993-12). The physico-chemical characterization was carried out by using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence (XRF). The XRD profile presented the main peaks of HA (JCPDS 860740) and the absorption bands of HA were identified by FTIR. The XRF results showed that the strontium concentration was close to the theoretical value. Regarding the cytotoxicity assays, the incorporation of strontium up to 5 mol. (%) to the HA did not affected dehydrogenase activity (XTT, 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide), membrane integrity (neutral red uptake) or DNA contend (incorporation of crystal violet), in relation to HA alone. In conclusion, hydroxyapatite containing from 0.5 to 5 mol. (%) of Sr was successfully produced and presented no cytotoxicity. |
| first_indexed | 2024-12-19T20:03:11Z |
| format | Article |
| id | doaj.art-762f5d731c3e4f6aa69161b6f7802588 |
| institution | Directory Open Access Journal |
| issn | 1516-1439 |
| language | English |
| last_indexed | 2024-12-19T20:03:11Z |
| publishDate | 2011-12-01 |
| publisher | Associação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol) |
| record_format | Article |
| series | Materials Research |
| spelling | doaj.art-762f5d731c3e4f6aa69161b6f78025882022-12-21T20:07:36ZengAssociação Brasileira de Metalurgia e Materiais (ABM); Associação Brasileira de Cerâmica (ABC); Associação Brasileira de Polímeros (ABPol)Materials Research1516-14392011-12-01144456460Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibilityDébora dos Santos TavaresCristiane Xavier ResendeMaíra Paiva QuitanLetícia de Oliveira CastroJosé Mauro GranjeiroGloria de Almeida SoaresThe aim of this work was to produce hydroxyapatite (HA) granules containing 0, 0.5, 1 and 5 mol. (%) of strontium (Sr), evaluate the physico-chemical properties and also the cytotoxicity by three different parameters of cell viability (ISO 10993-5, 10993-12). The physico-chemical characterization was carried out by using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence (XRF). The XRD profile presented the main peaks of HA (JCPDS 860740) and the absorption bands of HA were identified by FTIR. The XRF results showed that the strontium concentration was close to the theoretical value. Regarding the cytotoxicity assays, the incorporation of strontium up to 5 mol. (%) to the HA did not affected dehydrogenase activity (XTT, 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide), membrane integrity (neutral red uptake) or DNA contend (incorporation of crystal violet), in relation to HA alone. In conclusion, hydroxyapatite containing from 0.5 to 5 mol. (%) of Sr was successfully produced and presented no cytotoxicity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392011000400005strontiumhydroxyapatitecytotoxicity |
| spellingShingle | Débora dos Santos Tavares Cristiane Xavier Resende Maíra Paiva Quitan Letícia de Oliveira Castro José Mauro Granjeiro Gloria de Almeida Soares Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility Materials Research strontium hydroxyapatite cytotoxicity |
| title | Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility |
| title_full | Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility |
| title_fullStr | Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility |
| title_full_unstemmed | Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility |
| title_short | Incorporation of strontium up to 5 Mol. (%) to hydroxyapatite did not affect its cytocompatibility |
| title_sort | incorporation of strontium up to 5 mol to hydroxyapatite did not affect its cytocompatibility |
| topic | strontium hydroxyapatite cytotoxicity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392011000400005 |
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