Eukaryotic diversity of marine biofouling from coastal to offshore areas
Marine biofouling communities, including biofilms, are composed of many eukaryotes with high taxonomic and functional diversities. However, molecular characterization of eukaryotic diversity of marine biofouling has been barely developed due to the only recent interest in research areas such as mari...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.971939/full |
| _version_ | 1811196376778801152 |
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| author | Aurélie Portas Aurélie Portas Nolwenn Quillien Gérald Culioli Gérald Culioli Jean-François Briand |
| author_facet | Aurélie Portas Aurélie Portas Nolwenn Quillien Gérald Culioli Gérald Culioli Jean-François Briand |
| author_sort | Aurélie Portas |
| collection | DOAJ |
| description | Marine biofouling communities, including biofilms, are composed of many eukaryotes with high taxonomic and functional diversities. However, molecular characterization of eukaryotic diversity of marine biofouling has been barely developed due to the only recent interest in research areas such as marine renewable energies, antifouling technologies, or plastic pollution. The aim of this study was to compare the diversity and taxonomic composition of biofouling through different metabarcoding approaches used to detect the widest range of taxa from samples collected in several contrasted marine environments (French Atlantic and Mediterranean coasts). Thus, we assessed four DNA extraction methods and six primers pairs targeting the 18S rDNA gene (including the V1-V2, V4TAR, V4UNI, V7 and V9 regions) and the COI gene, the latter with two databases (BOLD and MIDORI). In addition the influence of primers selection was analyzed at three sites to compare geographic variations in eukaryotic diversity. Although none of the extraction methods greatly altered the community diversity or composition. we have observed that eukaryotic biofouling community diversity and structure varied depending on primers pairs, reference databases and sites. 18S rDNA regions allowed the detection of more taxa at the species level, including microeukaryotes, while the COI recovered more ASVs, but with a large proportion that remained taxonomically unassigned probably because BOLD and MIDORI specifically targeted metazoans. Interestingly, the spatial pattern obtained with both COI and 18S rDNA markers were similar showing that spatial selection occurred throughout a wide diversity of eukaryotic taxa. These results encouraged the use of these two complementary markers for future metabarcoding investigations but also highlighted the relevance of completing databases to enhance the identification of biofouling eukaryotes. |
| first_indexed | 2024-04-12T00:57:56Z |
| format | Article |
| id | doaj.art-76313e3118d34cf197f46d768a711009 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-12T00:57:56Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-76313e3118d34cf197f46d768a7110092022-12-22T03:54:34ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-10-01910.3389/fmars.2022.971939971939Eukaryotic diversity of marine biofouling from coastal to offshore areasAurélie Portas0Aurélie Portas1Nolwenn Quillien2Gérald Culioli3Gérald Culioli4Jean-François Briand5Laboratory Matériaux Polymères Interfaces Environnement Marin (MAPIEM), University of Toulon, EA 4323, Toulon, FranceFrance Energies Marines, Environmental Integration of Offshore Energy systems, Brest, FranceFrance Energies Marines, Environmental Integration of Offshore Energy systems, Brest, FranceLaboratory Matériaux Polymères Interfaces Environnement Marin (MAPIEM), University of Toulon, EA 4323, Toulon, FranceInstitut Méditerranéen de Biodiversité et d’Ecologie marine et continentale (IMBE), UMR CNRS-IRD-Avignon Université-Aix-Marseille Université, Avignon, FranceLaboratory Matériaux Polymères Interfaces Environnement Marin (MAPIEM), University of Toulon, EA 4323, Toulon, FranceMarine biofouling communities, including biofilms, are composed of many eukaryotes with high taxonomic and functional diversities. However, molecular characterization of eukaryotic diversity of marine biofouling has been barely developed due to the only recent interest in research areas such as marine renewable energies, antifouling technologies, or plastic pollution. The aim of this study was to compare the diversity and taxonomic composition of biofouling through different metabarcoding approaches used to detect the widest range of taxa from samples collected in several contrasted marine environments (French Atlantic and Mediterranean coasts). Thus, we assessed four DNA extraction methods and six primers pairs targeting the 18S rDNA gene (including the V1-V2, V4TAR, V4UNI, V7 and V9 regions) and the COI gene, the latter with two databases (BOLD and MIDORI). In addition the influence of primers selection was analyzed at three sites to compare geographic variations in eukaryotic diversity. Although none of the extraction methods greatly altered the community diversity or composition. we have observed that eukaryotic biofouling community diversity and structure varied depending on primers pairs, reference databases and sites. 18S rDNA regions allowed the detection of more taxa at the species level, including microeukaryotes, while the COI recovered more ASVs, but with a large proportion that remained taxonomically unassigned probably because BOLD and MIDORI specifically targeted metazoans. Interestingly, the spatial pattern obtained with both COI and 18S rDNA markers were similar showing that spatial selection occurred throughout a wide diversity of eukaryotic taxa. These results encouraged the use of these two complementary markers for future metabarcoding investigations but also highlighted the relevance of completing databases to enhance the identification of biofouling eukaryotes.https://www.frontiersin.org/articles/10.3389/fmars.2022.971939/fullbiofoulingmarine eukaryote diversitymetabarcodingdatabasespatial dissimilarity |
| spellingShingle | Aurélie Portas Aurélie Portas Nolwenn Quillien Gérald Culioli Gérald Culioli Jean-François Briand Eukaryotic diversity of marine biofouling from coastal to offshore areas Frontiers in Marine Science biofouling marine eukaryote diversity metabarcoding database spatial dissimilarity |
| title | Eukaryotic diversity of marine biofouling from coastal to offshore areas |
| title_full | Eukaryotic diversity of marine biofouling from coastal to offshore areas |
| title_fullStr | Eukaryotic diversity of marine biofouling from coastal to offshore areas |
| title_full_unstemmed | Eukaryotic diversity of marine biofouling from coastal to offshore areas |
| title_short | Eukaryotic diversity of marine biofouling from coastal to offshore areas |
| title_sort | eukaryotic diversity of marine biofouling from coastal to offshore areas |
| topic | biofouling marine eukaryote diversity metabarcoding database spatial dissimilarity |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.971939/full |
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