Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex
Intestinal barrier integrity is essential in order to maintain the homeostasis of mucosal functions and efficient defensive reactions against chemical and microbial challenges. An impairment of the intestinal barrier has been observed in several chronic diseases. The gut microbiota and its impact on...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/9/1295 |
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| author | Célia Chamignon Geoffroy Mallaret Julie Rivière Marthe Vilotte Sead Chadi Alejandra de Moreno de LeBlanc Jean Guy LeBlanc Frédéric Antonio Carvalho Marco Pane Pierre-Yves Mousset Philippe Langella Sophie Lafay Luis G. Bermúdez-Humarán |
| author_facet | Célia Chamignon Geoffroy Mallaret Julie Rivière Marthe Vilotte Sead Chadi Alejandra de Moreno de LeBlanc Jean Guy LeBlanc Frédéric Antonio Carvalho Marco Pane Pierre-Yves Mousset Philippe Langella Sophie Lafay Luis G. Bermúdez-Humarán |
| author_sort | Célia Chamignon |
| collection | DOAJ |
| description | Intestinal barrier integrity is essential in order to maintain the homeostasis of mucosal functions and efficient defensive reactions against chemical and microbial challenges. An impairment of the intestinal barrier has been observed in several chronic diseases. The gut microbiota and its impact on intestinal homeostasis is well described and numerous studies suggest the ability of some probiotic strains to protect the intestinal epithelial integrity and host homeostasis. In this work, we aimed to assess the beneficial effects of three <i>Lactobacillus</i> strains (<i>Lacticaseibacillus rhamnosus</i> LR04, <i>Lacticaseibacillus casei</i> LC03, and <i>Lactiplantibacillus plantarum</i> CNCM I-4459) and their mechanism of action in low-grade inflammation or neonatal maternal separation models in mice. We compared the impact of these strains to that of the well-known probiotic <i>Lacticaseibacillus rhamnosus</i> GG. Our results demonstrated that the three strains have the potential to restore the barrier functions by (i) increasing mucus production, (ii) restoring normal permeability, and (iii) modulating colonic hypersensitivity. Moreover, gene expression analysis of junctional proteins revealed the implication of Claudin 2 and Cingulin in the mechanisms that underlie the interactions between the strains and the host. Taken together, our data suggest that LR04, CNCM I-4459, and LC03 restore the functions of an impaired intestinal barrier. |
| first_indexed | 2024-03-10T22:59:23Z |
| format | Article |
| id | doaj.art-76329d8ab3eb4d3096ab9e22920653d7 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T22:59:23Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-76329d8ab3eb4d3096ab9e22920653d72023-11-19T09:44:59ZengMDPI AGBiomolecules2218-273X2023-08-01139129510.3390/biom13091295Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional ComplexCélia Chamignon0Geoffroy Mallaret1Julie Rivière2Marthe Vilotte3Sead Chadi4Alejandra de Moreno de LeBlanc5Jean Guy LeBlanc6Frédéric Antonio Carvalho7Marco Pane8Pierre-Yves Mousset9Philippe Langella10Sophie Lafay11Luis G. Bermúdez-Humarán12Institut National de Recherche pour l’Agriculture et l’Environnement (INRAE), Micalis Institut, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, FranceINSERM U1107 NeuroDol, University of Clermont Auvergne, 63001 Clermont-Ferrand, FranceInstitut National de Recherche pour l’Agriculture et l’Environnement (INRAE), Micalis Institut, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, FranceINRAE, GABI, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, FranceInstitut National de Recherche pour l’Agriculture et l’Environnement (INRAE), Micalis Institut, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, FranceCERELA-CONICET, San Miguel de Tucumán T4000ILC, Tucumán, ArgentinaCERELA-CONICET, San Miguel de Tucumán T4000ILC, Tucumán, ArgentinaINSERM U1107 NeuroDol, University of Clermont Auvergne, 63001 Clermont-Ferrand, FranceProbiotical Research, 28100 Novara, ItalyINDIGO Therapeutics, 33000 Bordeaux, FranceInstitut National de Recherche pour l’Agriculture et l’Environnement (INRAE), Micalis Institut, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, FranceINDIGO Therapeutics, 33000 Bordeaux, FranceInstitut National de Recherche pour l’Agriculture et l’Environnement (INRAE), Micalis Institut, AgroParisTech, University of Paris-Saclay, 78350 Jouy-en-Josas, FranceIntestinal barrier integrity is essential in order to maintain the homeostasis of mucosal functions and efficient defensive reactions against chemical and microbial challenges. An impairment of the intestinal barrier has been observed in several chronic diseases. The gut microbiota and its impact on intestinal homeostasis is well described and numerous studies suggest the ability of some probiotic strains to protect the intestinal epithelial integrity and host homeostasis. In this work, we aimed to assess the beneficial effects of three <i>Lactobacillus</i> strains (<i>Lacticaseibacillus rhamnosus</i> LR04, <i>Lacticaseibacillus casei</i> LC03, and <i>Lactiplantibacillus plantarum</i> CNCM I-4459) and their mechanism of action in low-grade inflammation or neonatal maternal separation models in mice. We compared the impact of these strains to that of the well-known probiotic <i>Lacticaseibacillus rhamnosus</i> GG. Our results demonstrated that the three strains have the potential to restore the barrier functions by (i) increasing mucus production, (ii) restoring normal permeability, and (iii) modulating colonic hypersensitivity. Moreover, gene expression analysis of junctional proteins revealed the implication of Claudin 2 and Cingulin in the mechanisms that underlie the interactions between the strains and the host. Taken together, our data suggest that LR04, CNCM I-4459, and LC03 restore the functions of an impaired intestinal barrier.https://www.mdpi.com/2218-273X/13/9/1295leaky gutchronic diseasesbarrier functionsprobiotics<i>Lactobacillus</i> |
| spellingShingle | Célia Chamignon Geoffroy Mallaret Julie Rivière Marthe Vilotte Sead Chadi Alejandra de Moreno de LeBlanc Jean Guy LeBlanc Frédéric Antonio Carvalho Marco Pane Pierre-Yves Mousset Philippe Langella Sophie Lafay Luis G. Bermúdez-Humarán Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex Biomolecules leaky gut chronic diseases barrier functions probiotics <i>Lactobacillus</i> |
| title | Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex |
| title_full | Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex |
| title_fullStr | Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex |
| title_full_unstemmed | Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex |
| title_short | Beneficial Effects of Lactobacilli Species on Intestinal Homeostasis in Low-Grade Inflammation and Stress Rodent Models and Their Implication in the Modulation of the Adhesive Junctional Complex |
| title_sort | beneficial effects of lactobacilli species on intestinal homeostasis in low grade inflammation and stress rodent models and their implication in the modulation of the adhesive junctional complex |
| topic | leaky gut chronic diseases barrier functions probiotics <i>Lactobacillus</i> |
| url | https://www.mdpi.com/2218-273X/13/9/1295 |
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