Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration
Post-traumatic lesions with transection of the facial nerve present limited functional outcome even after repair by gold-standard microsurgical techniques. Stem cell engraftment combined with surgical repair has been reported as a beneficial alternative. However, the best association between the sou...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2019-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/0963689718809090 |
| _version_ | 1818961470754390016 |
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| author | Larissa Vilela Pereira Ricardo Ferreira Bento Dayane B. Cruz Cláudia Marchi Raquel Salomone Jeanne Oiticicca Márcio Paulino Costa Luciana A. Haddad Regina Célia Mingroni-Netto Heloisa Juliana Zabeu Rossi Costa |
| author_facet | Larissa Vilela Pereira Ricardo Ferreira Bento Dayane B. Cruz Cláudia Marchi Raquel Salomone Jeanne Oiticicca Márcio Paulino Costa Luciana A. Haddad Regina Célia Mingroni-Netto Heloisa Juliana Zabeu Rossi Costa |
| author_sort | Larissa Vilela Pereira |
| collection | DOAJ |
| description | Post-traumatic lesions with transection of the facial nerve present limited functional outcome even after repair by gold-standard microsurgical techniques. Stem cell engraftment combined with surgical repair has been reported as a beneficial alternative. However, the best association between the source of stem cell and the nature of conduit, as well as the long-term postoperative cell viability are still matters of debate. We aimed to assess the functional and morphological effects of stem cells from human exfoliated deciduous teeth (SHED) in polyglycolic acid tube (PGAt) combined with autografting of rat facial nerve on repair after neurotmesis. The mandibular branch of rat facial nerve submitted to neurotmesis was repaired by autograft and PGAt filled with purified basement membrane matrix with or without SHED. Outcome variables were compound muscle action potential (CMAP) and axon morphometric. Animals from the SHED group had mean CMAP amplitudes and mean axonal diameters significantly higher than the control group ( p < 0.001). Mean axonal densities were significantly higher in the control group ( p = 0.004). The engrafted nerve segment resected 6 weeks after surgery presented cells of human origin that were positive for the Schwann cell marker (S100), indicating viability of transplanted SHED and a Schwann cell-like phenotype. We conclude that regeneration of the mandibular branch of the rat facial nerve was improved by SHED within PGAt. The stem cells integrated and remained viable in the neural tissue for 6 weeks since transplantation, and positive labeling for S100 Schwann-cell marker suggests cells initiated in vivo differentiation. |
| first_indexed | 2024-12-20T12:13:57Z |
| format | Article |
| id | doaj.art-763513f1007a4121b01894ccf6cfae24 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-20T12:13:57Z |
| publishDate | 2019-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-763513f1007a4121b01894ccf6cfae242022-12-21T19:41:12ZengSAGE PublishingCell Transplantation0963-68971555-38922019-01-012810.1177/0963689718809090Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve RegenerationLarissa Vilela Pereira0Ricardo Ferreira Bento1Dayane B. Cruz2Cláudia Marchi3Raquel Salomone4Jeanne Oiticicca5Márcio Paulino Costa6Luciana A. Haddad7Regina Célia Mingroni-Netto8Heloisa Juliana Zabeu Rossi Costa9 Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil Medical Investigations Laboratory of Otolaryngology/LIM 32, University of São Paulo, Brazil Human Genome and Stem-Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, Brazil Medical Investigations Laboratory of Otolaryngology/LIM 32, University of São Paulo, Brazil Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, Brazil Medical Investigations Laboratory of Otolaryngology/LIM 32, University of São Paulo, Brazil Department of Plastic Surgery, University of São Paulo, School of Medicine, Brazil Human Genome and Stem-Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, Brazil Human Genome and Stem-Cell Research Center, Department of Genetics and Evolutionary Biology, Biosciences Institute, University of São Paulo, Brazil Department of Ophthalmology and Otolaryngology, University of Sao Paulo, School of Medicine, BrazilPost-traumatic lesions with transection of the facial nerve present limited functional outcome even after repair by gold-standard microsurgical techniques. Stem cell engraftment combined with surgical repair has been reported as a beneficial alternative. However, the best association between the source of stem cell and the nature of conduit, as well as the long-term postoperative cell viability are still matters of debate. We aimed to assess the functional and morphological effects of stem cells from human exfoliated deciduous teeth (SHED) in polyglycolic acid tube (PGAt) combined with autografting of rat facial nerve on repair after neurotmesis. The mandibular branch of rat facial nerve submitted to neurotmesis was repaired by autograft and PGAt filled with purified basement membrane matrix with or without SHED. Outcome variables were compound muscle action potential (CMAP) and axon morphometric. Animals from the SHED group had mean CMAP amplitudes and mean axonal diameters significantly higher than the control group ( p < 0.001). Mean axonal densities were significantly higher in the control group ( p = 0.004). The engrafted nerve segment resected 6 weeks after surgery presented cells of human origin that were positive for the Schwann cell marker (S100), indicating viability of transplanted SHED and a Schwann cell-like phenotype. We conclude that regeneration of the mandibular branch of the rat facial nerve was improved by SHED within PGAt. The stem cells integrated and remained viable in the neural tissue for 6 weeks since transplantation, and positive labeling for S100 Schwann-cell marker suggests cells initiated in vivo differentiation.https://doi.org/10.1177/0963689718809090 |
| spellingShingle | Larissa Vilela Pereira Ricardo Ferreira Bento Dayane B. Cruz Cláudia Marchi Raquel Salomone Jeanne Oiticicca Márcio Paulino Costa Luciana A. Haddad Regina Célia Mingroni-Netto Heloisa Juliana Zabeu Rossi Costa Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration Cell Transplantation |
| title | Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration |
| title_full | Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration |
| title_fullStr | Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration |
| title_full_unstemmed | Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration |
| title_short | Stem Cells from Human Exfoliated Deciduous Teeth (SHED) Differentiate and Promote Facial Nerve Regeneration |
| title_sort | stem cells from human exfoliated deciduous teeth shed differentiate and promote facial nerve regeneration |
| url | https://doi.org/10.1177/0963689718809090 |
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