Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia
Dopamine and serotonin receptors and transporters play an essential role in the pathophysiology of schizophrenia; changes in their expression have been reported in neurons and leukocytes. Each antipsychotic induces a unique pattern in leukocyte function and phenotype. However, the use of polytherapy...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/1424-8247/17/2/167 |
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| author | Samantha Alvarez-Herrera Mauricio Rosel Vales Gilberto Pérez-Sánchez Enrique Becerril-Villanueva Yvonne Flores-Medina José Luis Maldonado-García Ricardo Saracco-Alvarez Raúl Escamilla Lenin Pavón |
| author_facet | Samantha Alvarez-Herrera Mauricio Rosel Vales Gilberto Pérez-Sánchez Enrique Becerril-Villanueva Yvonne Flores-Medina José Luis Maldonado-García Ricardo Saracco-Alvarez Raúl Escamilla Lenin Pavón |
| author_sort | Samantha Alvarez-Herrera |
| collection | DOAJ |
| description | Dopamine and serotonin receptors and transporters play an essential role in the pathophysiology of schizophrenia; changes in their expression have been reported in neurons and leukocytes. Each antipsychotic induces a unique pattern in leukocyte function and phenotype. However, the use of polytherapy to treat schizophrenia makes it challenging to determine the specific effects of risperidone on peripheral blood mononuclear cells (PBMCs). The aim of this study was to evaluate the changes in the expression of D<sub>3</sub>, D<sub>5</sub>, DAT, 5-HT<sub>2A</sub>, and SERT in PBMCs from healthy volunteers (HV), drug-naive patients with schizophrenia (PWS), drug-free PWS, and PWS treated with risperidone for up to 40 weeks using quantitative PCR. Our study revealed elevated mRNA levels of D<sub>3</sub>, DAT, 5-HT<sub>2A</sub>, and SERT in unmedicated PWS. Treatment with risperidone led to a reduction only in the expression of 5-HT<sub>2A</sub> and SERT. Furthermore, we observed a moderate correlation between 5-HT<sub>2A</sub> expression and the positive and negative syndrome scale (PANSS), as well as SERT expression and PANSS scale. We also found a moderate correlation between 5-HT<sub>2A</sub> and SERT expression and the positive subscale. The duration of risperidone consumption had a significant negative correlation with the expression of 5-HT<sub>2A</sub> and SERT. Our study introduces the measurement of 5-HT<sub>2A</sub> and SERT expression in PBMCs as a useful parameter for assessing the response to risperidone in PWS. |
| first_indexed | 2024-03-07T22:17:58Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-07T22:17:58Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-7635f1ea971a425493cde8bebaaf062b2024-02-23T15:30:33ZengMDPI AGPharmaceuticals1424-82472024-01-0117216710.3390/ph17020167Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with SchizophreniaSamantha Alvarez-Herrera0Mauricio Rosel Vales1Gilberto Pérez-Sánchez2Enrique Becerril-Villanueva3Yvonne Flores-Medina4José Luis Maldonado-García5Ricardo Saracco-Alvarez6Raúl Escamilla7Lenin Pavón8Laboratorio de Psicoinmunología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoClínica de Esquizofrenia, Dirección de Servicios Clínicos, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoLaboratorio de Psicoinmunología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoLaboratorio de Psicoinmunología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoSubdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoDepartamemto de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, MexicoSubdirección de Investigaciones Clínicas, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoSubdirección de Consulta Externa, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoLaboratorio de Psicoinmunología, Dirección de Investigaciones en Neurociencias, Instituto Nacional de Psiquiatría Ramón de la Fuente Muñíz, Mexico City 14370, MexicoDopamine and serotonin receptors and transporters play an essential role in the pathophysiology of schizophrenia; changes in their expression have been reported in neurons and leukocytes. Each antipsychotic induces a unique pattern in leukocyte function and phenotype. However, the use of polytherapy to treat schizophrenia makes it challenging to determine the specific effects of risperidone on peripheral blood mononuclear cells (PBMCs). The aim of this study was to evaluate the changes in the expression of D<sub>3</sub>, D<sub>5</sub>, DAT, 5-HT<sub>2A</sub>, and SERT in PBMCs from healthy volunteers (HV), drug-naive patients with schizophrenia (PWS), drug-free PWS, and PWS treated with risperidone for up to 40 weeks using quantitative PCR. Our study revealed elevated mRNA levels of D<sub>3</sub>, DAT, 5-HT<sub>2A</sub>, and SERT in unmedicated PWS. Treatment with risperidone led to a reduction only in the expression of 5-HT<sub>2A</sub> and SERT. Furthermore, we observed a moderate correlation between 5-HT<sub>2A</sub> expression and the positive and negative syndrome scale (PANSS), as well as SERT expression and PANSS scale. We also found a moderate correlation between 5-HT<sub>2A</sub> and SERT expression and the positive subscale. The duration of risperidone consumption had a significant negative correlation with the expression of 5-HT<sub>2A</sub> and SERT. Our study introduces the measurement of 5-HT<sub>2A</sub> and SERT expression in PBMCs as a useful parameter for assessing the response to risperidone in PWS.https://www.mdpi.com/1424-8247/17/2/167schizophreniadopamine receptorsserotonin receptorsdopamine transporterserotonin transporterrisperidone |
| spellingShingle | Samantha Alvarez-Herrera Mauricio Rosel Vales Gilberto Pérez-Sánchez Enrique Becerril-Villanueva Yvonne Flores-Medina José Luis Maldonado-García Ricardo Saracco-Alvarez Raúl Escamilla Lenin Pavón Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia Pharmaceuticals schizophrenia dopamine receptors serotonin receptors dopamine transporter serotonin transporter risperidone |
| title | Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia |
| title_full | Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia |
| title_fullStr | Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia |
| title_full_unstemmed | Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia |
| title_short | Risperidone Decreases Expression of Serotonin Receptor-2A (5-HT2A) and Serotonin Transporter (SERT) but Not Dopamine Receptors and Dopamine Transporter (DAT) in PBMCs from Patients with Schizophrenia |
| title_sort | risperidone decreases expression of serotonin receptor 2a 5 ht2a and serotonin transporter sert but not dopamine receptors and dopamine transporter dat in pbmcs from patients with schizophrenia |
| topic | schizophrenia dopamine receptors serotonin receptors dopamine transporter serotonin transporter risperidone |
| url | https://www.mdpi.com/1424-8247/17/2/167 |
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