The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis
Introduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood microbiome have been reported to be altered in patient...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Spanish |
| Published: |
Elsevier
2024-03-01
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| Series: | Nefrología |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S021169952300139X |
| _version_ | 1797277358942584832 |
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| author | Ricardo Araujo Ana Merino-Ribas Luciano Pereira Joana Campos Nádia Silva Inês Soares Alencastre Manuel Pestana Benedita Sampaio-Maia |
| author_facet | Ricardo Araujo Ana Merino-Ribas Luciano Pereira Joana Campos Nádia Silva Inês Soares Alencastre Manuel Pestana Benedita Sampaio-Maia |
| author_sort | Ricardo Araujo |
| collection | DOAJ |
| description | Introduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood microbiome have been reported to be altered in patients with chronic kidney disease (CKD), and specifically in peritoneal dialysis (PD) patients. Still, there are currently no studies describing the urogenital microbiome in CKD-PD patients. In this study we characterized the urobiome profile in 46 PD patients and analyzed its clinical and inflammatory parameters. Materials and methods: Mid-stream urine, fecal and blood samples were collected from 46 patients undergoing PD at Centro Hospitalar Universitário de São João (CHUSJ) in Porto, Portugal. Exclusion criteria were age under 18 years old, inability to give informed consent, history of infection in the last three months, and antibiotic intake in the last three months. The microbiome communities were analyzed by amplification and sequencing of the V3–V4 region of the bacterial 16S rRNA gene. Correlations with the patients’ clinical data and inflammatory profile were performed. Results: CKD-PD patients presented a unique urobiome profile dominated by Bacillota, Actinomycetota and Pseudomonadota and characterized by a lower Shannon diversity than fecal and blood microbiome. The taxonomic profiles of urogenital samples were organized in multiple subtypes dominated by populations of Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, being similar to other non-PD-CKD patients. Gender, sCD14, residual diuresis and history of peritonitis were significantly associated to variations in the urobiome. Although not reaching statistical significance, diabetes and the time on PD also showed association with particular taxonomic groups. Depletion of Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus or Dermabacter populations correlated with CKD-PD patients with history of diabetes, history of peritonitis and altered levels of sCD14. Conclusions: Our results highlight urogenital microbiome as a potential partner and/or marker in the overall health state of CKD-PD patients. Resumen: Introducción y objetivos: Diabetes, dislipemia, edad avanzada, género, infecciones del tracto urinario y toma reciente de antibióticos, entre otras, se han asociado a una disminución en la riqueza del urobioma y a otras fluctuaciones de dicho microbioma.Recientemente, se han descrito alteraciones en losmicrobiomas intestinal y en sangreen pacientes con enfermedad renal crónica (ERC) y, específicamente, en pacientes en diálisis peritoneal (DP).A pesar de ello, aún no existen estudios que describan el microbioma urogenital en pacientes en DP. En el presente trabajo, caracterizamos el urobioma en 46 pacientes en DP. Pacientes y métodos: Se recogieron muestras de orina (micción espontánea), heces y sangre de 46 pacientes en DP del Centro HospitalarUniversitário de São João en Oporto, Portugal. Los criterios de exclusión fueron edad menor a 18 años, incapacidad para entenderel consentimiento informado, e historia de infección y toma de antibióticos en los últimos 3 meses. Las comunidades microbiológicas fueron analizadas por amplificación y secuenciación de las regiones V3-V4 del 16S rRNA bacteriano. Se realizaron correlaciones con los datos clínicos y el perfil inflamatorio de los pacientes. Resultados: Los pacientes en DP presentaron un urobioma único dominado por Bacillota, Actinomycetota yPseudomonadota, y caracterizado por una menor diversidad de Shannon que los microbiomas en sangre e intestinal. Los perfiles taxonómicos de las muestras urogenitales se organizaron en múltiples subtipos dominados por poblaciones de Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, siendo similar al descrito para otros pacientes con ERC no en DP.Género, factor sCD14, diuresis residual yantecedentes de peritonitis se asociaron de forma significativa a cambios en el urobioma. Diabetes y tiempo en DP también se asociaron con grupos taxonómicos específicos, aunque no de forma estadísticamente significativa. La deplección de Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus o Dermabacterse correlacionó con antecedentes de diabetes, historia de peritonitis y niveles alterados de sCD14. Conclusiones: Nuestros resultados subrayan la importancia del microbioma urogenital como potencial biomarcadordel estado de salud de nuestros pacientes en DP. |
| first_indexed | 2024-03-07T15:47:32Z |
| format | Article |
| id | doaj.art-7638229d88d44eb1ab004317ebc62d8d |
| institution | Directory Open Access Journal |
| issn | 0211-6995 |
| language | Spanish |
| last_indexed | 2024-03-07T15:47:32Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Nefrología |
| spelling | doaj.art-7638229d88d44eb1ab004317ebc62d8d2024-03-05T04:29:30ZspaElsevierNefrología0211-69952024-03-01442194203The urogenital microbiome in chronic kidney disease patients on peritoneal dialysisRicardo Araujo0Ana Merino-Ribas1Luciano Pereira2Joana Campos3Nádia Silva4Inês Soares Alencastre5Manuel Pestana6Benedita Sampaio-Maia7Nephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; Corresponding author.Nephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; Nephrology Department, Hospital Universitari de Girona Doctor Josep Trueta, Girona, Spain; Universitat Autònoma de Barcelona, Barcelona, SpainNephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; Nephrology Department, Centro Hospitalar Universitário de São João, Porto, PortugalNephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, PortugalNephrology Department, Centro Hospitalar Universitário de São João, Porto, PortugalNephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, PortugalNephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; Nephrology Department, Centro Hospitalar Universitário de São João, Porto, PortugalNephrology & Infectious Diseases R&D Group, i3S – Instituto de Investigação e Inovação em Saúde, INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; Faculdade de Medicina Dentária, Universidade do Porto, Porto, PortugalIntroduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood microbiome have been reported to be altered in patients with chronic kidney disease (CKD), and specifically in peritoneal dialysis (PD) patients. Still, there are currently no studies describing the urogenital microbiome in CKD-PD patients. In this study we characterized the urobiome profile in 46 PD patients and analyzed its clinical and inflammatory parameters. Materials and methods: Mid-stream urine, fecal and blood samples were collected from 46 patients undergoing PD at Centro Hospitalar Universitário de São João (CHUSJ) in Porto, Portugal. Exclusion criteria were age under 18 years old, inability to give informed consent, history of infection in the last three months, and antibiotic intake in the last three months. The microbiome communities were analyzed by amplification and sequencing of the V3–V4 region of the bacterial 16S rRNA gene. Correlations with the patients’ clinical data and inflammatory profile were performed. Results: CKD-PD patients presented a unique urobiome profile dominated by Bacillota, Actinomycetota and Pseudomonadota and characterized by a lower Shannon diversity than fecal and blood microbiome. The taxonomic profiles of urogenital samples were organized in multiple subtypes dominated by populations of Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, being similar to other non-PD-CKD patients. Gender, sCD14, residual diuresis and history of peritonitis were significantly associated to variations in the urobiome. Although not reaching statistical significance, diabetes and the time on PD also showed association with particular taxonomic groups. Depletion of Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus or Dermabacter populations correlated with CKD-PD patients with history of diabetes, history of peritonitis and altered levels of sCD14. Conclusions: Our results highlight urogenital microbiome as a potential partner and/or marker in the overall health state of CKD-PD patients. Resumen: Introducción y objetivos: Diabetes, dislipemia, edad avanzada, género, infecciones del tracto urinario y toma reciente de antibióticos, entre otras, se han asociado a una disminución en la riqueza del urobioma y a otras fluctuaciones de dicho microbioma.Recientemente, se han descrito alteraciones en losmicrobiomas intestinal y en sangreen pacientes con enfermedad renal crónica (ERC) y, específicamente, en pacientes en diálisis peritoneal (DP).A pesar de ello, aún no existen estudios que describan el microbioma urogenital en pacientes en DP. En el presente trabajo, caracterizamos el urobioma en 46 pacientes en DP. Pacientes y métodos: Se recogieron muestras de orina (micción espontánea), heces y sangre de 46 pacientes en DP del Centro HospitalarUniversitário de São João en Oporto, Portugal. Los criterios de exclusión fueron edad menor a 18 años, incapacidad para entenderel consentimiento informado, e historia de infección y toma de antibióticos en los últimos 3 meses. Las comunidades microbiológicas fueron analizadas por amplificación y secuenciación de las regiones V3-V4 del 16S rRNA bacteriano. Se realizaron correlaciones con los datos clínicos y el perfil inflamatorio de los pacientes. Resultados: Los pacientes en DP presentaron un urobioma único dominado por Bacillota, Actinomycetota yPseudomonadota, y caracterizado por una menor diversidad de Shannon que los microbiomas en sangre e intestinal. Los perfiles taxonómicos de las muestras urogenitales se organizaron en múltiples subtipos dominados por poblaciones de Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, siendo similar al descrito para otros pacientes con ERC no en DP.Género, factor sCD14, diuresis residual yantecedentes de peritonitis se asociaron de forma significativa a cambios en el urobioma. Diabetes y tiempo en DP también se asociaron con grupos taxonómicos específicos, aunque no de forma estadísticamente significativa. La deplección de Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus o Dermabacterse correlacionó con antecedentes de diabetes, historia de peritonitis y niveles alterados de sCD14. Conclusiones: Nuestros resultados subrayan la importancia del microbioma urogenital como potencial biomarcadordel estado de salud de nuestros pacientes en DP.http://www.sciencedirect.com/science/article/pii/S021169952300139XMicrobioma urogenitalUrobiomaMicrobioma humanoMicrobioma intestinalInfección del tracto urinarioDiálisis peritoneal |
| spellingShingle | Ricardo Araujo Ana Merino-Ribas Luciano Pereira Joana Campos Nádia Silva Inês Soares Alencastre Manuel Pestana Benedita Sampaio-Maia The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis Nefrología Microbioma urogenital Urobioma Microbioma humano Microbioma intestinal Infección del tracto urinario Diálisis peritoneal |
| title | The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis |
| title_full | The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis |
| title_fullStr | The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis |
| title_full_unstemmed | The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis |
| title_short | The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis |
| title_sort | urogenital microbiome in chronic kidney disease patients on peritoneal dialysis |
| topic | Microbioma urogenital Urobioma Microbioma humano Microbioma intestinal Infección del tracto urinario Diálisis peritoneal |
| url | http://www.sciencedirect.com/science/article/pii/S021169952300139X |
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