Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic
Abstract Background The Asian tiger mosquito, Aedes (Stegomyia) albopictus (Skuse) is an important worldwide invasive species and can be a locally important vector of chikungunya, dengue and, potentially, Zika. This species is native to Southeast Asia where populations thrive in both temperate and t...
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| Format: | Article |
| Language: | English |
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BMC
2019-10-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3740-0 |
| _version_ | 1819045781591556096 |
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| author | Maysa Tiemi Motoki Dina Madera Fonseca Elliott Frederic Miot Bruna Demari-Silva Phoutmany Thammavong Somsanith Chonephetsarath Nothasine Phommavanh Jeffrey Conrad Hertz Pattamaporn Kittayapong Paul Trevor Brey Sebastien Marcombe |
| author_facet | Maysa Tiemi Motoki Dina Madera Fonseca Elliott Frederic Miot Bruna Demari-Silva Phoutmany Thammavong Somsanith Chonephetsarath Nothasine Phommavanh Jeffrey Conrad Hertz Pattamaporn Kittayapong Paul Trevor Brey Sebastien Marcombe |
| author_sort | Maysa Tiemi Motoki |
| collection | DOAJ |
| description | Abstract Background The Asian tiger mosquito, Aedes (Stegomyia) albopictus (Skuse) is an important worldwide invasive species and can be a locally important vector of chikungunya, dengue and, potentially, Zika. This species is native to Southeast Asia where populations thrive in both temperate and tropical climates. A better understanding of the population structure of Ae. albopictus in Lao PDR is very important in order to support the implementation of strategies for diseases prevention and vector control. In the present study, we investigated the genetic variability of Ae. albopictus across a north-south transect in Lao PDR. Methods We used variability in a 1337-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1), to assess the population structure of Ae. albopictus in Lao PDR. For context, we also examined variability at the same genetic locus in samples of Ae. albopictus from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA. Results We observed very high levels of genetic polymorphism with 46 novel haplotypes in Ae. albopictus from 9 localities in Lao PDR and Thailand populations. Significant differences were observed between the Luangnamtha population and other locations in Lao PDR. However, we found no evidence of isolation by distance. There was overall little genetic structure indicating ongoing and frequent gene flow among populations or a recent population expansion. Indeed, the neutrality test supported population expansion in Laotian Ae. albopictus and mismatch distribution analyses showed a lack of low frequency alleles, a pattern often seen in bottlenecked populations. When samples from Lao PDR were analyzed together with samples from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA, phylogenetic network and Bayesian cluster analysis showed that most populations from tropical/subtropical regions are more genetically related to each other, than populations from temperate regions. Similarly, most populations from temperate regions are more genetically related to each other, than those from tropical/subtropical regions. Conclusions Aedes albopictus in Lao PDR are genetically related to populations from tropical/subtropical regions (i.e. Thailand, Singapore, and California and Texas in the USA). The extensive gene flow among locations in Lao PDR indicates that local control is undermined by repeated introductions from untreated sites. |
| first_indexed | 2024-12-21T10:34:02Z |
| format | Article |
| id | doaj.art-7638a1f16f6341c3829f8d722f6020d0 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-21T10:34:02Z |
| publishDate | 2019-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-7638a1f16f6341c3829f8d722f6020d02022-12-21T19:07:08ZengBMCParasites & Vectors1756-33052019-10-0112111210.1186/s13071-019-3740-0Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic RepublicMaysa Tiemi Motoki0Dina Madera Fonseca1Elliott Frederic Miot2Bruna Demari-Silva3Phoutmany Thammavong4Somsanith Chonephetsarath5Nothasine Phommavanh6Jeffrey Conrad Hertz7Pattamaporn Kittayapong8Paul Trevor Brey9Sebastien Marcombe10Medical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosCenter for Vector Biology and Department of Entomology, Rutgers UniversityMedical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosDepartment of Epidemiology, Faculty of Public Health, University of Sao PauloMedical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosMedical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosMedical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosU.S. Naval Medical Research Unit TWOMahidol UniversityMedical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosMedical Entomology and Vector Borne Disease Unit, Institut Pasteur du LaosAbstract Background The Asian tiger mosquito, Aedes (Stegomyia) albopictus (Skuse) is an important worldwide invasive species and can be a locally important vector of chikungunya, dengue and, potentially, Zika. This species is native to Southeast Asia where populations thrive in both temperate and tropical climates. A better understanding of the population structure of Ae. albopictus in Lao PDR is very important in order to support the implementation of strategies for diseases prevention and vector control. In the present study, we investigated the genetic variability of Ae. albopictus across a north-south transect in Lao PDR. Methods We used variability in a 1337-bp fragment of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1), to assess the population structure of Ae. albopictus in Lao PDR. For context, we also examined variability at the same genetic locus in samples of Ae. albopictus from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA. Results We observed very high levels of genetic polymorphism with 46 novel haplotypes in Ae. albopictus from 9 localities in Lao PDR and Thailand populations. Significant differences were observed between the Luangnamtha population and other locations in Lao PDR. However, we found no evidence of isolation by distance. There was overall little genetic structure indicating ongoing and frequent gene flow among populations or a recent population expansion. Indeed, the neutrality test supported population expansion in Laotian Ae. albopictus and mismatch distribution analyses showed a lack of low frequency alleles, a pattern often seen in bottlenecked populations. When samples from Lao PDR were analyzed together with samples from Thailand, China, Taiwan, Japan, Singapore, Italy and the USA, phylogenetic network and Bayesian cluster analysis showed that most populations from tropical/subtropical regions are more genetically related to each other, than populations from temperate regions. Similarly, most populations from temperate regions are more genetically related to each other, than those from tropical/subtropical regions. Conclusions Aedes albopictus in Lao PDR are genetically related to populations from tropical/subtropical regions (i.e. Thailand, Singapore, and California and Texas in the USA). The extensive gene flow among locations in Lao PDR indicates that local control is undermined by repeated introductions from untreated sites.http://link.springer.com/article/10.1186/s13071-019-3740-0Aedes albopictuscox1 geneGenetic populationLao PDR |
| spellingShingle | Maysa Tiemi Motoki Dina Madera Fonseca Elliott Frederic Miot Bruna Demari-Silva Phoutmany Thammavong Somsanith Chonephetsarath Nothasine Phommavanh Jeffrey Conrad Hertz Pattamaporn Kittayapong Paul Trevor Brey Sebastien Marcombe Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic Parasites & Vectors Aedes albopictus cox1 gene Genetic population Lao PDR |
| title | Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic |
| title_full | Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic |
| title_fullStr | Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic |
| title_full_unstemmed | Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic |
| title_short | Population genetics of Aedes albopictus (Diptera: Culicidae) in its native range in Lao People’s Democratic Republic |
| title_sort | population genetics of aedes albopictus diptera culicidae in its native range in lao people s democratic republic |
| topic | Aedes albopictus cox1 gene Genetic population Lao PDR |
| url | http://link.springer.com/article/10.1186/s13071-019-3740-0 |
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