Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters
2,3-Butanediol (2,3-BD) is a reagent with remarkable commercial use as a platform chemical in numerous industries. The present study aims to determine the capabilities of non-pathogenic and cellulolytic <i>Bacillus licheniformis</i> 24 as a 2,3-BD producer. By applying the Plackett–Burma...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/3/118 |
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| author | Lidia Tsigoriyna Dimitar Ganchev Penka Petrova Kaloyan Petrov |
| author_facet | Lidia Tsigoriyna Dimitar Ganchev Penka Petrova Kaloyan Petrov |
| author_sort | Lidia Tsigoriyna |
| collection | DOAJ |
| description | 2,3-Butanediol (2,3-BD) is a reagent with remarkable commercial use as a platform chemical in numerous industries. The present study aims to determine the capabilities of non-pathogenic and cellulolytic <i>Bacillus licheniformis</i> 24 as a 2,3-BD producer. By applying the Plackett–Burman design and response surface methodology through central composite design (CCD), a complex optimization of medium and process parameters was conducted. Thus, among ten studied factors of medium content, four components were evaluated with a significant positive effect on 2,3-BD formation. Their optimal values for 2,3-BD production (yeast extract, 13.38 g/L; tryptone, 6.41 g/L; K<sub>2</sub>HPO<sub>4</sub>, 4.2 g/L; MgSO<sub>4</sub>, 0.32 g/L), as well as the optimal temperature (37.8 °C), pH (6.23) and aeration rate (3.68 vvm) were predicted by CCD experiments and validated in a series of batch processes. In optimized batch fermentation of 200 g/L of glucose 91.23 g/L of 2,3-BD was obtained, with the overall productivity of 1.94 g/L/h and yield of 0.488 g/g. To reveal the maximum 2,3-BD tolerance of <i>B. licheniformis</i> 24, fed-batch fermentation was carried out. The obtained 138.8 g/L of 2,3-BD with a yield of 0.479 g/g and productivity of 1.16 g/L/h ranks the strain among the best 2,3-BD producers. |
| first_indexed | 2024-03-10T07:41:38Z |
| format | Article |
| id | doaj.art-763a57c17ffa4c358122d2d418b62baa |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T07:41:38Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-763a57c17ffa4c358122d2d418b62baa2023-11-22T12:59:24ZengMDPI AGFermentation2311-56372021-07-017311810.3390/fermentation7030118Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological ParametersLidia Tsigoriyna0Dimitar Ganchev1Penka Petrova2Kaloyan Petrov3Institute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Microbiology, Bulgarian Academy of Sciences, 1113 Sofia, BulgariaInstitute of Chemical Engineering, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria2,3-Butanediol (2,3-BD) is a reagent with remarkable commercial use as a platform chemical in numerous industries. The present study aims to determine the capabilities of non-pathogenic and cellulolytic <i>Bacillus licheniformis</i> 24 as a 2,3-BD producer. By applying the Plackett–Burman design and response surface methodology through central composite design (CCD), a complex optimization of medium and process parameters was conducted. Thus, among ten studied factors of medium content, four components were evaluated with a significant positive effect on 2,3-BD formation. Their optimal values for 2,3-BD production (yeast extract, 13.38 g/L; tryptone, 6.41 g/L; K<sub>2</sub>HPO<sub>4</sub>, 4.2 g/L; MgSO<sub>4</sub>, 0.32 g/L), as well as the optimal temperature (37.8 °C), pH (6.23) and aeration rate (3.68 vvm) were predicted by CCD experiments and validated in a series of batch processes. In optimized batch fermentation of 200 g/L of glucose 91.23 g/L of 2,3-BD was obtained, with the overall productivity of 1.94 g/L/h and yield of 0.488 g/g. To reveal the maximum 2,3-BD tolerance of <i>B. licheniformis</i> 24, fed-batch fermentation was carried out. The obtained 138.8 g/L of 2,3-BD with a yield of 0.479 g/g and productivity of 1.16 g/L/h ranks the strain among the best 2,3-BD producers.https://www.mdpi.com/2311-5637/7/3/1182,3-butanediol<i>Bacillus licheniformis</i>Plackett–Burman designresponse surface methodology |
| spellingShingle | Lidia Tsigoriyna Dimitar Ganchev Penka Petrova Kaloyan Petrov Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters Fermentation 2,3-butanediol <i>Bacillus licheniformis</i> Plackett–Burman design response surface methodology |
| title | Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters |
| title_full | Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters |
| title_fullStr | Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters |
| title_full_unstemmed | Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters |
| title_short | Highly Efficient 2,3-Butanediol Production by <i>Bacillus licheniformis</i> via Complex Optimization of Nutritional and Technological Parameters |
| title_sort | highly efficient 2 3 butanediol production by i bacillus licheniformis i via complex optimization of nutritional and technological parameters |
| topic | 2,3-butanediol <i>Bacillus licheniformis</i> Plackett–Burman design response surface methodology |
| url | https://www.mdpi.com/2311-5637/7/3/118 |
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