Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers
IntroductionDietary nutrient content is crucial for energy metabolism and development of gut microbiota. Herein, this study aimed to explore the effects of fat-to-fiber ratios on nutrient transporter, energy metabolism and gut microbiota when ingredients composition was altered.MethodsA total of 240...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1298262/full |
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| author | Qiuyu Jiang Lihua Zhao Zhibin Ban Zhibin Ban Bingkun Zhang |
| author_facet | Qiuyu Jiang Lihua Zhao Zhibin Ban Zhibin Ban Bingkun Zhang |
| author_sort | Qiuyu Jiang |
| collection | DOAJ |
| description | IntroductionDietary nutrient content is crucial for energy metabolism and development of gut microbiota. Herein, this study aimed to explore the effects of fat-to-fiber ratios on nutrient transporter, energy metabolism and gut microbiota when ingredients composition was altered.MethodsA total of 240 as-hatched broiler chickens were randomly assigned into three groups including low fat-high dietary fiber (LF-HD), medium fat-medium dietary fiber (MF-MD) and high fat-low dietary fiber (HF-LD), with diets being iso-protein, and broilers were offered the same commercial diets from 21 to 42 d. The data were analyzed using one-way ANOVA of SPSS.Results and DiscussionResults showed that HF-LD diet significantly increased glucose content and decreased triglyceride in serum of broilers (p < 0.05). The mRNA abundance of jejunal gene involved in glucose transporter and tricarboxylic acid (TCA) cycle was significantly increased in broilers fed with HF-LD diets. Compared with LF-HD, HF-LD had a lower abundance of Anaerofilum and CHKCI001, and an increased proportion of beneficial bacteria such as Alistipes, Catenibacillus, Intestinimonas, Lactobacillus, and Peptococcus (p < 0.05). Functional prediction of these microbial changes indicated that HF-LD diet drove caecal microbiota to participate in carbohydrate metabolism and TCA cycle (p < 0.05). Dietary HF-LD-induced microbiota changes were positively correlated with growth performance of broilers (p < 0.05). Therefore, HF-LD diet increased glucose transporters and energy metabolism in intestine and shaped microbial structure and metabolic pathways, which may benefit the growth performance of broilers. |
| first_indexed | 2024-04-24T22:40:32Z |
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| id | doaj.art-763bab18f0554480969ddd3d4ca551c4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T22:40:32Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-763bab18f0554480969ddd3d4ca551c42024-03-19T04:33:02ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.12982621298262Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilersQiuyu Jiang0Lihua Zhao1Zhibin Ban2Zhibin Ban3Bingkun Zhang4State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaLaboratory of Animal Nutrition Metabolism, Jilin Academy of Agricultural Sciences, Jilin, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaIntroductionDietary nutrient content is crucial for energy metabolism and development of gut microbiota. Herein, this study aimed to explore the effects of fat-to-fiber ratios on nutrient transporter, energy metabolism and gut microbiota when ingredients composition was altered.MethodsA total of 240 as-hatched broiler chickens were randomly assigned into three groups including low fat-high dietary fiber (LF-HD), medium fat-medium dietary fiber (MF-MD) and high fat-low dietary fiber (HF-LD), with diets being iso-protein, and broilers were offered the same commercial diets from 21 to 42 d. The data were analyzed using one-way ANOVA of SPSS.Results and DiscussionResults showed that HF-LD diet significantly increased glucose content and decreased triglyceride in serum of broilers (p < 0.05). The mRNA abundance of jejunal gene involved in glucose transporter and tricarboxylic acid (TCA) cycle was significantly increased in broilers fed with HF-LD diets. Compared with LF-HD, HF-LD had a lower abundance of Anaerofilum and CHKCI001, and an increased proportion of beneficial bacteria such as Alistipes, Catenibacillus, Intestinimonas, Lactobacillus, and Peptococcus (p < 0.05). Functional prediction of these microbial changes indicated that HF-LD diet drove caecal microbiota to participate in carbohydrate metabolism and TCA cycle (p < 0.05). Dietary HF-LD-induced microbiota changes were positively correlated with growth performance of broilers (p < 0.05). Therefore, HF-LD diet increased glucose transporters and energy metabolism in intestine and shaped microbial structure and metabolic pathways, which may benefit the growth performance of broilers.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1298262/fullenergyfatnon-starch polysaccharidemicrobiotabroiler |
| spellingShingle | Qiuyu Jiang Lihua Zhao Zhibin Ban Zhibin Ban Bingkun Zhang Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers Frontiers in Microbiology energy fat non-starch polysaccharide microbiota broiler |
| title | Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers |
| title_full | Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers |
| title_fullStr | Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers |
| title_full_unstemmed | Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers |
| title_short | Different fat-to-fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers |
| title_sort | different fat to fiber ratios by changing wheat inclusion level impact energy metabolism and microbial structure of broilers |
| topic | energy fat non-starch polysaccharide microbiota broiler |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1298262/full |
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