Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae
Abstract Background For decades, plastic has been a valuable global product due to its convenience and low price. For example, polyethylene terephthalate (PET) was one of the most popular materials for disposable bottles due to its beneficial properties, namely impact resistance, high clarity, and l...
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Microbial Cell Factories |
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| Online Access: | http://link.springer.com/article/10.1186/s12934-020-01355-8 |
| _version_ | 1811214873664684032 |
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| author | Ji Won Kim Su-Bin Park Quynh-Giao Tran Dae-Hyun Cho Dong-Yun Choi Yong Jae Lee Hee-Sik Kim |
| author_facet | Ji Won Kim Su-Bin Park Quynh-Giao Tran Dae-Hyun Cho Dong-Yun Choi Yong Jae Lee Hee-Sik Kim |
| author_sort | Ji Won Kim |
| collection | DOAJ |
| description | Abstract Background For decades, plastic has been a valuable global product due to its convenience and low price. For example, polyethylene terephthalate (PET) was one of the most popular materials for disposable bottles due to its beneficial properties, namely impact resistance, high clarity, and light weight. Increasing demand of plastic resulted in indiscriminate disposal by consumers, causing severe accumulation of plastic wastes. Because of this, scientists have made great efforts to find a way to biologically treat plastic wastes. As a result, a novel plastic degradation enzyme, PETase, which can hydrolyze PET, was discovered in Ideonella sakaiensis 201-F6 in 2016. Results A green algae, Chlamydomonas reinhardtii, which produces PETase, was developed for this study. Two representative strains (C. reinhardtii CC-124 and CC-503) were examined, and we found that CC-124 could express PETase well. To verify the catalytic activity of PETase produced by C. reinhardtii, cell lysate of the transformant and PET samples were co-incubated at 30 °C for up to 4 weeks. After incubation, terephthalic acid (TPA), i.e. the fully-degraded form of PET, was detected by high performance liquid chromatography analysis. Additionally, morphological changes, such as holes and dents on the surface of PET film, were observed using scanning electron microscopy. Conclusions A PET hydrolyzing enzyme, PETase, was successfully expressed in C. reinhardtii, and its catalytic activity was demonstrated. To the best of our knowledge, this is the first case of PETase expression in green algae. |
| first_indexed | 2024-04-12T06:12:49Z |
| format | Article |
| id | doaj.art-763f006354d14883bb92c89ec1797286 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-12T06:12:49Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-763f006354d14883bb92c89ec17972862022-12-22T03:44:39ZengBMCMicrobial Cell Factories1475-28592020-04-011911910.1186/s12934-020-01355-8Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgaeJi Won Kim0Su-Bin Park1Quynh-Giao Tran2Dae-Hyun Cho3Dong-Yun Choi4Yong Jae Lee5Hee-Sik Kim6Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Abstract Background For decades, plastic has been a valuable global product due to its convenience and low price. For example, polyethylene terephthalate (PET) was one of the most popular materials for disposable bottles due to its beneficial properties, namely impact resistance, high clarity, and light weight. Increasing demand of plastic resulted in indiscriminate disposal by consumers, causing severe accumulation of plastic wastes. Because of this, scientists have made great efforts to find a way to biologically treat plastic wastes. As a result, a novel plastic degradation enzyme, PETase, which can hydrolyze PET, was discovered in Ideonella sakaiensis 201-F6 in 2016. Results A green algae, Chlamydomonas reinhardtii, which produces PETase, was developed for this study. Two representative strains (C. reinhardtii CC-124 and CC-503) were examined, and we found that CC-124 could express PETase well. To verify the catalytic activity of PETase produced by C. reinhardtii, cell lysate of the transformant and PET samples were co-incubated at 30 °C for up to 4 weeks. After incubation, terephthalic acid (TPA), i.e. the fully-degraded form of PET, was detected by high performance liquid chromatography analysis. Additionally, morphological changes, such as holes and dents on the surface of PET film, were observed using scanning electron microscopy. Conclusions A PET hydrolyzing enzyme, PETase, was successfully expressed in C. reinhardtii, and its catalytic activity was demonstrated. To the best of our knowledge, this is the first case of PETase expression in green algae.http://link.springer.com/article/10.1186/s12934-020-01355-8Polyethylene terephthalate (PET)PET hydrolase (PETase)Plastic degradationMicroalgaeChlamydomonas reinhardtiiBioremediation |
| spellingShingle | Ji Won Kim Su-Bin Park Quynh-Giao Tran Dae-Hyun Cho Dong-Yun Choi Yong Jae Lee Hee-Sik Kim Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae Microbial Cell Factories Polyethylene terephthalate (PET) PET hydrolase (PETase) Plastic degradation Microalgae Chlamydomonas reinhardtii Bioremediation |
| title | Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae |
| title_full | Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae |
| title_fullStr | Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae |
| title_full_unstemmed | Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae |
| title_short | Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae |
| title_sort | functional expression of polyethylene terephthalate degrading enzyme petase in green microalgae |
| topic | Polyethylene terephthalate (PET) PET hydrolase (PETase) Plastic degradation Microalgae Chlamydomonas reinhardtii Bioremediation |
| url | http://link.springer.com/article/10.1186/s12934-020-01355-8 |
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