Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress
Global warming is expected to reduce the nutrient concentration in the upper ocean and affect the physiology of marine diatoms, but the underlying molecular mechanisms controlling these physiological changes are currently unknown. To understand these mechanisms, here we investigated iTRAQ based prot...
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| Format: | Article |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.554832/full |
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| author | Satheeswaran Thangaraj Satheesh Kumar Palanisamy Guicheng Zhang Guicheng Zhang Jun Sun |
| author_facet | Satheeswaran Thangaraj Satheesh Kumar Palanisamy Guicheng Zhang Guicheng Zhang Jun Sun |
| author_sort | Satheeswaran Thangaraj |
| collection | DOAJ |
| description | Global warming is expected to reduce the nutrient concentration in the upper ocean and affect the physiology of marine diatoms, but the underlying molecular mechanisms controlling these physiological changes are currently unknown. To understand these mechanisms, here we investigated iTRAQ based proteomic profiling of diatom Skeletonema dohrnii in a multifactorial experimental with a combining change of temperature and silicate concentrations. In total, 3369 differently abundant proteins were detected in four different environmental conditions, and the function of all proteins was identified using Gene Ontology and KEGG pathway analysis. For discriminating the proteome variation among samples, multivariate statistical analysis (PCA, PLS-DA) was performed by comparing the protein ratio differences. Further, performing pathway analysis on diatom proteomes, we here demonstrated downregulation of photosynthesis, carbon metabolism, and ribosome biogenesis in the cellular process that leads to decrease the oxidoreductase activity and affects the cell cycle of the diatom. Using PLS-DA VIP score plot analysis, we identified 15 protein biomarkers for discriminating studied samples. Of these, five proteins or gene (rbcL, PRK, atpB, DNA-binding, and signal transduction) identified as key biomarkers, induced by temperature and silicate stress in diatom metabolism. Our results show that proteomic finger-printing of S. dohrnii with different environmental conditions adds biological information that strengthens marine phytoplankton proteome analysis. |
| first_indexed | 2024-12-20T11:59:17Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T11:59:17Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-76424f5f1e944619bcce9ef25827a3452022-12-21T19:41:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-01-011110.3389/fmicb.2020.554832554832Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental StressSatheeswaran Thangaraj0Satheesh Kumar Palanisamy1Guicheng Zhang2Guicheng Zhang3Jun Sun4College of Marine Science and Technology, China University of Geosciences, Wuhan, ChinaDepartment of Zoology, School of Natural Science, Ryan Institute, National University of Ireland, Galway, IrelandResearch Center for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin, ChinaTianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin, ChinaCollege of Marine Science and Technology, China University of Geosciences, Wuhan, ChinaGlobal warming is expected to reduce the nutrient concentration in the upper ocean and affect the physiology of marine diatoms, but the underlying molecular mechanisms controlling these physiological changes are currently unknown. To understand these mechanisms, here we investigated iTRAQ based proteomic profiling of diatom Skeletonema dohrnii in a multifactorial experimental with a combining change of temperature and silicate concentrations. In total, 3369 differently abundant proteins were detected in four different environmental conditions, and the function of all proteins was identified using Gene Ontology and KEGG pathway analysis. For discriminating the proteome variation among samples, multivariate statistical analysis (PCA, PLS-DA) was performed by comparing the protein ratio differences. Further, performing pathway analysis on diatom proteomes, we here demonstrated downregulation of photosynthesis, carbon metabolism, and ribosome biogenesis in the cellular process that leads to decrease the oxidoreductase activity and affects the cell cycle of the diatom. Using PLS-DA VIP score plot analysis, we identified 15 protein biomarkers for discriminating studied samples. Of these, five proteins or gene (rbcL, PRK, atpB, DNA-binding, and signal transduction) identified as key biomarkers, induced by temperature and silicate stress in diatom metabolism. Our results show that proteomic finger-printing of S. dohrnii with different environmental conditions adds biological information that strengthens marine phytoplankton proteome analysis.https://www.frontiersin.org/articles/10.3389/fmicb.2020.554832/fullglobal warmingphotosynthesiscarbon metabolismnutrient stratificationsiTRAQ-proteomicsclimate change |
| spellingShingle | Satheeswaran Thangaraj Satheesh Kumar Palanisamy Guicheng Zhang Guicheng Zhang Jun Sun Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress Frontiers in Microbiology global warming photosynthesis carbon metabolism nutrient stratifications iTRAQ-proteomics climate change |
| title | Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress |
| title_full | Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress |
| title_fullStr | Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress |
| title_full_unstemmed | Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress |
| title_short | Quantitative Proteomic Profiling of Marine Diatom Skeletonema dohrnii in Response to Temperature and Silicate Induced Environmental Stress |
| title_sort | quantitative proteomic profiling of marine diatom skeletonema dohrnii in response to temperature and silicate induced environmental stress |
| topic | global warming photosynthesis carbon metabolism nutrient stratifications iTRAQ-proteomics climate change |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.554832/full |
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