Meibum lipid composition in Asians with dry eye disease.
Previous lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite the higher prevalence of dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to the alterations in lipid profiles in relation to DTS onset and progression...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3197196?pdf=render |
| _version_ | 1828847247117254656 |
|---|---|
| author | Sin Man Lam Louis Tong Siew Sian Yong Bowen Li Shyam S Chaurasia Guanghou Shui Markus R Wenk |
| author_facet | Sin Man Lam Louis Tong Siew Sian Yong Bowen Li Shyam S Chaurasia Guanghou Shui Markus R Wenk |
| author_sort | Sin Man Lam |
| collection | DOAJ |
| description | Previous lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite the higher prevalence of dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to the alterations in lipid profiles in relation to DTS onset and progression is also lacking and warrants comprehensive experimental analysis.We examined the meibum lipidome of 27 DTS patients and 10 control subjects for a total of 256 lipid species from 12 major lipid classes, including cholesteryl ester (CE), wax ester (WE), triacylglyceride (TAG), (O-acyl)-ω-hydroxy fatty acid (OAHFA), glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG) and sphingolipids (sphingomyelin, SM; ceramide, Cer; glucosylceramide, GluCer; dihexosylceramide, DihexCer). Neutral lipids were analysed using high-performance liquid-chromatography coupled with mass spectrometry (HPLC/MS) and tandem mass spectrometry (MS/MS) was used for the qualitative and quantitative analysis of polar lipid species. DTS patients were classified into three severity groups (i.e. mild, moderate and severe) based on the ocular surface disease index (OSDI). A significantly lower level of TAG (p<0.05) was observed in patients under the moderate category compared to the mild category. Notably, a number of OAHFA species displayed consistently decreasing levels that correlate with increasing disease severity. An attempt was also made to investigate the changes in meibum lipid profiles of DTS patients compared to normal individuals classified based on OSDI score. Several unsaturated TAG and PC species were found at significantly higher levels (p<0.05) in patients than controls.The current study presents, for the first time, a comprehensive lipidome of meibum from individuals of an Asian ethnicity, which can potentially offer new insights into the higher prevalence of DTS observed amongst Asian populations. This study also represents an attempt towards identification of lipid species in meibum which could serve as marker for DTS. |
| first_indexed | 2024-12-12T22:05:53Z |
| format | Article |
| id | doaj.art-7643e14730854185a93fa4fb563b0256 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T22:05:53Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7643e14730854185a93fa4fb563b02562022-12-22T00:10:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2433910.1371/journal.pone.0024339Meibum lipid composition in Asians with dry eye disease.Sin Man LamLouis TongSiew Sian YongBowen LiShyam S ChaurasiaGuanghou ShuiMarkus R WenkPrevious lipidomic analyses of the human meibum had largely focused on individuals from non-Asian populations, despite the higher prevalence of dysfunctional tear syndrome (DTS) observed across Asia. Information pertaining to the alterations in lipid profiles in relation to DTS onset and progression is also lacking and warrants comprehensive experimental analysis.We examined the meibum lipidome of 27 DTS patients and 10 control subjects for a total of 256 lipid species from 12 major lipid classes, including cholesteryl ester (CE), wax ester (WE), triacylglyceride (TAG), (O-acyl)-ω-hydroxy fatty acid (OAHFA), glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG) and sphingolipids (sphingomyelin, SM; ceramide, Cer; glucosylceramide, GluCer; dihexosylceramide, DihexCer). Neutral lipids were analysed using high-performance liquid-chromatography coupled with mass spectrometry (HPLC/MS) and tandem mass spectrometry (MS/MS) was used for the qualitative and quantitative analysis of polar lipid species. DTS patients were classified into three severity groups (i.e. mild, moderate and severe) based on the ocular surface disease index (OSDI). A significantly lower level of TAG (p<0.05) was observed in patients under the moderate category compared to the mild category. Notably, a number of OAHFA species displayed consistently decreasing levels that correlate with increasing disease severity. An attempt was also made to investigate the changes in meibum lipid profiles of DTS patients compared to normal individuals classified based on OSDI score. Several unsaturated TAG and PC species were found at significantly higher levels (p<0.05) in patients than controls.The current study presents, for the first time, a comprehensive lipidome of meibum from individuals of an Asian ethnicity, which can potentially offer new insights into the higher prevalence of DTS observed amongst Asian populations. This study also represents an attempt towards identification of lipid species in meibum which could serve as marker for DTS.http://europepmc.org/articles/PMC3197196?pdf=render |
| spellingShingle | Sin Man Lam Louis Tong Siew Sian Yong Bowen Li Shyam S Chaurasia Guanghou Shui Markus R Wenk Meibum lipid composition in Asians with dry eye disease. PLoS ONE |
| title | Meibum lipid composition in Asians with dry eye disease. |
| title_full | Meibum lipid composition in Asians with dry eye disease. |
| title_fullStr | Meibum lipid composition in Asians with dry eye disease. |
| title_full_unstemmed | Meibum lipid composition in Asians with dry eye disease. |
| title_short | Meibum lipid composition in Asians with dry eye disease. |
| title_sort | meibum lipid composition in asians with dry eye disease |
| url | http://europepmc.org/articles/PMC3197196?pdf=render |
| work_keys_str_mv | AT sinmanlam meibumlipidcompositioninasianswithdryeyedisease AT louistong meibumlipidcompositioninasianswithdryeyedisease AT siewsianyong meibumlipidcompositioninasianswithdryeyedisease AT bowenli meibumlipidcompositioninasianswithdryeyedisease AT shyamschaurasia meibumlipidcompositioninasianswithdryeyedisease AT guanghoushui meibumlipidcompositioninasianswithdryeyedisease AT markusrwenk meibumlipidcompositioninasianswithdryeyedisease |