Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids
The ever-increasing global energy demand, juxtaposed with critical concerns about greenhouse gas emissions, emphatically underscores the urgency to pivot toward sustainable and eco-friendly energy alternatives. Tapping into microbial metabolism for clean energy generation stands out as a particularl...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/11/984 |
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| author | Kai Wang Zhuoheng Wu Jingping Du Yining Liu Zehao Zhu Pan Feng Haoran Bi Yang Zhang Yanhui Liu Biqiang Chen Meng Wang Tianwei Tan |
| author_facet | Kai Wang Zhuoheng Wu Jingping Du Yining Liu Zehao Zhu Pan Feng Haoran Bi Yang Zhang Yanhui Liu Biqiang Chen Meng Wang Tianwei Tan |
| author_sort | Kai Wang |
| collection | DOAJ |
| description | The ever-increasing global energy demand, juxtaposed with critical concerns about greenhouse gas emissions, emphatically underscores the urgency to pivot toward sustainable and eco-friendly energy alternatives. Tapping into microbial metabolism for clean energy generation stands out as a particularly promising avenue in this endeavor. Given this backdrop, we delved deeply into the metabolic engineering potential of <i>Saccharomyces cerevisiae</i>, thereby aiming for the bioconversion of formate and acetate—both CO<sub>2</sub> derivatives—into free fatty acids (FFAs) as precursors for biofuel production. Our study not only elucidated the metabolic pathways within <i>S. cerevisiae</i> that are tailored for efficient formate and acetate utilization but also shone a light on the meticulous optimization strategies that amplify FFA synthesis. The engineered strains, under refined conditions, exhibited up to an 8-fold increase in an FFA titer, thus reaching a production level of 6.6 g/L, which showcases the potential of microbial metabolism in clean energy generation. Our findings offer a promising step toward harnessing microbial metabolism for sustainable energy production, thereby bridging the gap between waste carbon utilization and greener fuel alternatives. |
| first_indexed | 2024-03-09T16:50:55Z |
| format | Article |
| id | doaj.art-7648e9d3bafc4cba8b4136370104bcd2 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T16:50:55Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-7648e9d3bafc4cba8b4136370104bcd22023-11-24T14:41:26ZengMDPI AGFermentation2311-56372023-11-0191198410.3390/fermentation9110984Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty AcidsKai Wang0Zhuoheng Wu1Jingping Du2Yining Liu3Zehao Zhu4Pan Feng5Haoran Bi6Yang Zhang7Yanhui Liu8Biqiang Chen9Meng Wang10Tianwei Tan11National Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaNational Energy Research Center for Biorefinery, Beijing University of Chemical Technology, Beijing 100029, ChinaThe ever-increasing global energy demand, juxtaposed with critical concerns about greenhouse gas emissions, emphatically underscores the urgency to pivot toward sustainable and eco-friendly energy alternatives. Tapping into microbial metabolism for clean energy generation stands out as a particularly promising avenue in this endeavor. Given this backdrop, we delved deeply into the metabolic engineering potential of <i>Saccharomyces cerevisiae</i>, thereby aiming for the bioconversion of formate and acetate—both CO<sub>2</sub> derivatives—into free fatty acids (FFAs) as precursors for biofuel production. Our study not only elucidated the metabolic pathways within <i>S. cerevisiae</i> that are tailored for efficient formate and acetate utilization but also shone a light on the meticulous optimization strategies that amplify FFA synthesis. The engineered strains, under refined conditions, exhibited up to an 8-fold increase in an FFA titer, thus reaching a production level of 6.6 g/L, which showcases the potential of microbial metabolism in clean energy generation. Our findings offer a promising step toward harnessing microbial metabolism for sustainable energy production, thereby bridging the gap between waste carbon utilization and greener fuel alternatives.https://www.mdpi.com/2311-5637/9/11/984free fatty acids<i>Saccharomyces cerevisiae</i>formate and acetatemetabolic engineeringfed-batch fermentation |
| spellingShingle | Kai Wang Zhuoheng Wu Jingping Du Yining Liu Zehao Zhu Pan Feng Haoran Bi Yang Zhang Yanhui Liu Biqiang Chen Meng Wang Tianwei Tan Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids Fermentation free fatty acids <i>Saccharomyces cerevisiae</i> formate and acetate metabolic engineering fed-batch fermentation |
| title | Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids |
| title_full | Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids |
| title_fullStr | Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids |
| title_full_unstemmed | Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids |
| title_short | Metabolic Engineering of <i>Saccharomyces cerevisiae</i> for Conversion of Formate and Acetate into Free Fatty Acids |
| title_sort | metabolic engineering of i saccharomyces cerevisiae i for conversion of formate and acetate into free fatty acids |
| topic | free fatty acids <i>Saccharomyces cerevisiae</i> formate and acetate metabolic engineering fed-batch fermentation |
| url | https://www.mdpi.com/2311-5637/9/11/984 |
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