Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq
Seed size play a significant role in maize yield production. Two maize inbred lines with distinct seed size and weight, V671 (a large-seed inbred line) and Mc (a small-seed inbred line), were investigated by RNA-seq at 14 days after pollination (DAP), when maize endosperm undergoes an active transit...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311918619562 |
| _version_ | 1818602861007732736 |
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| author | Yu-qian ZHOU Qin-yang WANG Hai-liang ZHAO Dian-ming GONG Chuan-long SUN Xue-mei REN Zhong-xiang LIU Hai-jun HE Fa-zhan QIU |
| author_facet | Yu-qian ZHOU Qin-yang WANG Hai-liang ZHAO Dian-ming GONG Chuan-long SUN Xue-mei REN Zhong-xiang LIU Hai-jun HE Fa-zhan QIU |
| author_sort | Yu-qian ZHOU |
| collection | DOAJ |
| description | Seed size play a significant role in maize yield production. Two maize inbred lines with distinct seed size and weight, V671 (a large-seed inbred line) and Mc (a small-seed inbred line), were investigated by RNA-seq at 14 days after pollination (DAP), when maize endosperm undergoes an active transition from mitosis to storage accumulation. RNA-seq expression data showed that the small-seed line Mc had a higher storage accumulation activity, whereas the large-seed kernel line V671 possessed a higher DNA synthesis activity. An investigation of the pattern of increasing kernel width at serial DAPs showed that V671 experienced an increased kernel width later than did Mc, but the rate and duration of increase were longer in V671. SDS-PAGE of the storage proteins and quantitative RT-PCR of cell cycle-related genes and indole-3-acetic (IAA) synthesis genes certified that the transition from mitosis to storage accumulation starts earlier in Mc. We hypothesized that the difference in the mitosis-to-storage accumulation transition accounts for the larger seed size in V671 vs. Mc. |
| first_indexed | 2024-12-16T13:14:00Z |
| format | Article |
| id | doaj.art-764c229c5c094f0ca6febb8b918c5b0e |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-16T13:14:00Z |
| publishDate | 2018-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-764c229c5c094f0ca6febb8b918c5b0e2022-12-21T22:30:31ZengElsevierJournal of Integrative Agriculture2095-31192018-07-0117715741584Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seqYu-qian ZHOU0Qin-yang WANG1Hai-liang ZHAO2Dian-ming GONG3Chuan-long SUN4Xue-mei REN5Zhong-xiang LIU6Hai-jun HE7Fa-zhan QIU8Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. ChinaCrop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R. ChinaCrop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, P.R. ChinaNational Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R. China; Correspondence QIU Fa-zhan, Tel: +86-27-87286870, Fax: +86-27-87281006Seed size play a significant role in maize yield production. Two maize inbred lines with distinct seed size and weight, V671 (a large-seed inbred line) and Mc (a small-seed inbred line), were investigated by RNA-seq at 14 days after pollination (DAP), when maize endosperm undergoes an active transition from mitosis to storage accumulation. RNA-seq expression data showed that the small-seed line Mc had a higher storage accumulation activity, whereas the large-seed kernel line V671 possessed a higher DNA synthesis activity. An investigation of the pattern of increasing kernel width at serial DAPs showed that V671 experienced an increased kernel width later than did Mc, but the rate and duration of increase were longer in V671. SDS-PAGE of the storage proteins and quantitative RT-PCR of cell cycle-related genes and indole-3-acetic (IAA) synthesis genes certified that the transition from mitosis to storage accumulation starts earlier in Mc. We hypothesized that the difference in the mitosis-to-storage accumulation transition accounts for the larger seed size in V671 vs. Mc.http://www.sciencedirect.com/science/article/pii/S2095311918619562maize (Zea mays L.)kernelRNA-seqmitosis-storage accumulationtransitionendosperm |
| spellingShingle | Yu-qian ZHOU Qin-yang WANG Hai-liang ZHAO Dian-ming GONG Chuan-long SUN Xue-mei REN Zhong-xiang LIU Hai-jun HE Fa-zhan QIU Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq Journal of Integrative Agriculture maize (Zea mays L.) kernel RNA-seq mitosis-storage accumulation transition endosperm |
| title | Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq |
| title_full | Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq |
| title_fullStr | Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq |
| title_full_unstemmed | Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq |
| title_short | Unravelling transcriptome changes between two distinct maize inbred lines using RNA-seq |
| title_sort | unravelling transcriptome changes between two distinct maize inbred lines using rna seq |
| topic | maize (Zea mays L.) kernel RNA-seq mitosis-storage accumulation transition endosperm |
| url | http://www.sciencedirect.com/science/article/pii/S2095311918619562 |
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