Applications and challenges of rhodopsin-based optogenetics in biomedicine
Optogenetics is an emerging bioengineering technology that has been rapidly developed in recent years by cross-integrating optics, genetic engineering, electrophysiology, software control, and other disciplines. Since the first demonstration of the millisecond neuromodulation ability of the channelr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.966772/full |
| _version_ | 1798000462500200448 |
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| author | Hanci Zhang Hui Fang Deqiang Liu Yiming Zhang Joseph Adu-Amankwaah Jinxiang Yuan Jinxiang Yuan Jinxiang Yuan Rubin Tan Jianping Zhu |
| author_facet | Hanci Zhang Hui Fang Deqiang Liu Yiming Zhang Joseph Adu-Amankwaah Jinxiang Yuan Jinxiang Yuan Jinxiang Yuan Rubin Tan Jianping Zhu |
| author_sort | Hanci Zhang |
| collection | DOAJ |
| description | Optogenetics is an emerging bioengineering technology that has been rapidly developed in recent years by cross-integrating optics, genetic engineering, electrophysiology, software control, and other disciplines. Since the first demonstration of the millisecond neuromodulation ability of the channelrhodopsin-2 (ChR2), the application of optogenetic technology in basic life science research has been rapidly progressed, especially in neurobiology, which has driven the development of the discipline. As the optogenetic tool protein, microbial rhodopsins have been continuously explored, modified, and optimized, with many variants becoming available, with structural characteristics and functions that are highly diversified. Their applicability has been broadened, encouraging more researchers and clinicians to utilize optogenetics technology in research. In this review, we summarize the species and variant types of the most important class of tool proteins in optogenetic techniques, the microbial rhodopsins, and review the current applications of optogenetics based on rhodopsin qualitative light in biology and other fields. We also review the challenges facing this technology, to ultimately provide an in-depth technical reference to support the application of optogenetics in translational and clinical research. |
| first_indexed | 2024-04-11T11:20:40Z |
| format | Article |
| id | doaj.art-764c690aa26148a6a325a3b213d5a85d |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-11T11:20:40Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-764c690aa26148a6a325a3b213d5a85d2022-12-22T04:27:04ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-09-011610.3389/fnins.2022.966772966772Applications and challenges of rhodopsin-based optogenetics in biomedicineHanci Zhang0Hui Fang1Deqiang Liu2Yiming Zhang3Joseph Adu-Amankwaah4Jinxiang Yuan5Jinxiang Yuan6Jinxiang Yuan7Rubin Tan8Jianping Zhu9College of Life Sciences, Shandong Normal University, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaDepartment of Physiology, Basic Medical School, Xuzhou Medical University, Xuzhou, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaCollaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, ChinaLin He’s Academician Workstation of New Medicine and Clinical Translation, Jining Medical University, Jining, ChinaDepartment of Physiology, Basic Medical School, Xuzhou Medical University, Xuzhou, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaOptogenetics is an emerging bioengineering technology that has been rapidly developed in recent years by cross-integrating optics, genetic engineering, electrophysiology, software control, and other disciplines. Since the first demonstration of the millisecond neuromodulation ability of the channelrhodopsin-2 (ChR2), the application of optogenetic technology in basic life science research has been rapidly progressed, especially in neurobiology, which has driven the development of the discipline. As the optogenetic tool protein, microbial rhodopsins have been continuously explored, modified, and optimized, with many variants becoming available, with structural characteristics and functions that are highly diversified. Their applicability has been broadened, encouraging more researchers and clinicians to utilize optogenetics technology in research. In this review, we summarize the species and variant types of the most important class of tool proteins in optogenetic techniques, the microbial rhodopsins, and review the current applications of optogenetics based on rhodopsin qualitative light in biology and other fields. We also review the challenges facing this technology, to ultimately provide an in-depth technical reference to support the application of optogenetics in translational and clinical research.https://www.frontiersin.org/articles/10.3389/fnins.2022.966772/fulloptogeneticsmicrobial rhodopsinAlzheimer’s diseaseParkinson’s diseasecardiac optogeneticsoptical cochlear implant |
| spellingShingle | Hanci Zhang Hui Fang Deqiang Liu Yiming Zhang Joseph Adu-Amankwaah Jinxiang Yuan Jinxiang Yuan Jinxiang Yuan Rubin Tan Jianping Zhu Applications and challenges of rhodopsin-based optogenetics in biomedicine Frontiers in Neuroscience optogenetics microbial rhodopsin Alzheimer’s disease Parkinson’s disease cardiac optogenetics optical cochlear implant |
| title | Applications and challenges of rhodopsin-based optogenetics in biomedicine |
| title_full | Applications and challenges of rhodopsin-based optogenetics in biomedicine |
| title_fullStr | Applications and challenges of rhodopsin-based optogenetics in biomedicine |
| title_full_unstemmed | Applications and challenges of rhodopsin-based optogenetics in biomedicine |
| title_short | Applications and challenges of rhodopsin-based optogenetics in biomedicine |
| title_sort | applications and challenges of rhodopsin based optogenetics in biomedicine |
| topic | optogenetics microbial rhodopsin Alzheimer’s disease Parkinson’s disease cardiac optogenetics optical cochlear implant |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.966772/full |
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