Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro
Periodontitis is an inflamation of supporting tissues on teeth that became the main cause of Prophyromonas gingivalis (P. gingivalis) bacteria. Treatment of periodontitis is by giving antibacterial agent therapy. Plants that have antibacterial effects one are Eleutherine bulbosa (UEB) bulbs from the...
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| Format: | Article |
| Language: | English |
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Mulawarman University
2018-06-01
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| Series: | Journal of Tropical Pharmacy and Chemistry |
| Subjects: | |
| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/151 |
| _version_ | 1797637459488538624 |
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| author | Siti Nur Azizah Sinar Yani Sjarif Ismail Masyudhi Masyudhi Endang Sawitri |
| author_facet | Siti Nur Azizah Sinar Yani Sjarif Ismail Masyudhi Masyudhi Endang Sawitri |
| author_sort | Siti Nur Azizah |
| collection | DOAJ |
| description | Periodontitis is an inflamation of supporting tissues on teeth that became the main cause of Prophyromonas gingivalis (P. gingivalis) bacteria. Treatment of periodontitis is by giving antibacterial agent therapy. Plants that have antibacterial effects one are Eleutherine bulbosa (UEB) bulbs from the Iridaceae family. This plant has been used for ethnobotany boils drugs and has been known to have antibacterial activity toward against intestinal pathogen bacteria that is anaerobic Gram Negative, but still unknown its activity against on oral pathogenic bacteria such as P. gingivalis. The purpose of this study was to know the effect of UEB extract on the growth of P. gingivalis bacteria as in vitro. UEB is taken from agriculture center in Samarinda city. UEB Simplisia was tested for antibacterial activity using Kirby-Bauer disc diffusion method on BHI-A media supplemented with vitamin K and hemin. The bacteria which used were P. gingivalis ATCC® 33277. Blank disc 6 mm with nine concentrations attached to BHI-A medium, incubated for 24 hours at 37 °C in an anaerobic atmosphere, then sprayed MTT (3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenylttetrazoliumbromide) reagent and read a few moments later. For comparison, then used Chlorhexidine gluconate (CHX) 2 mg/ml. The statistical analysis with t-test and there was significant different if p<0.05. The result showed that the higher concentration of UEB ethanol extract will increase the growth inhibition zone of P. gingivalis bacteria and reach maximum at 10 mg/ml concentration. The result of t-test showed no difference significant of P. gingivalis bacterial inhibition zone in the treatment group of 7.5 mg/ml and 10 mg/ml concentration on CHX group. This study proves UEB ethanol extract can inhibit the growth of P. gingivalis bacteria.
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| first_indexed | 2024-03-11T12:48:40Z |
| format | Article |
| id | doaj.art-764d7371c46f476baf77fe98e64936f2 |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:48:40Z |
| publishDate | 2018-06-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-764d7371c46f476baf77fe98e64936f22023-11-04T06:12:14ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902018-06-014310.25026/jtpc.v4i3.151Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In VitroSiti Nur Azizah0Sinar Yani1Sjarif Ismail2Masyudhi Masyudhi3Endang Sawitri4Dentistry Study Program, Faculty of Medicine, Mulawarman University, IndonesiaOral Biology Laboratory, Dentistry Study Program, Faculty of Medicine, Mulawarman University, IndonesiaResearch Center on Drugs and Public Health, Mulawarman University, IndonesiaLaboratory of Microbiology, Faculty of Medicine, Mulawarman University, IndonesiaPhysiology Laboratory, Faculty of Medicine, Mulawarman University, IndonesiaPeriodontitis is an inflamation of supporting tissues on teeth that became the main cause of Prophyromonas gingivalis (P. gingivalis) bacteria. Treatment of periodontitis is by giving antibacterial agent therapy. Plants that have antibacterial effects one are Eleutherine bulbosa (UEB) bulbs from the Iridaceae family. This plant has been used for ethnobotany boils drugs and has been known to have antibacterial activity toward against intestinal pathogen bacteria that is anaerobic Gram Negative, but still unknown its activity against on oral pathogenic bacteria such as P. gingivalis. The purpose of this study was to know the effect of UEB extract on the growth of P. gingivalis bacteria as in vitro. UEB is taken from agriculture center in Samarinda city. UEB Simplisia was tested for antibacterial activity using Kirby-Bauer disc diffusion method on BHI-A media supplemented with vitamin K and hemin. The bacteria which used were P. gingivalis ATCC® 33277. Blank disc 6 mm with nine concentrations attached to BHI-A medium, incubated for 24 hours at 37 °C in an anaerobic atmosphere, then sprayed MTT (3-[4,5-Dimethylthiazole-2-yl]-2,5-diphenylttetrazoliumbromide) reagent and read a few moments later. For comparison, then used Chlorhexidine gluconate (CHX) 2 mg/ml. The statistical analysis with t-test and there was significant different if p<0.05. The result showed that the higher concentration of UEB ethanol extract will increase the growth inhibition zone of P. gingivalis bacteria and reach maximum at 10 mg/ml concentration. The result of t-test showed no difference significant of P. gingivalis bacterial inhibition zone in the treatment group of 7.5 mg/ml and 10 mg/ml concentration on CHX group. This study proves UEB ethanol extract can inhibit the growth of P. gingivalis bacteria. https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/151Eleutherine bulbosaProphyromonas gingivalisInhibition ZoneAntibacterial AgentsPeriodontitis |
| spellingShingle | Siti Nur Azizah Sinar Yani Sjarif Ismail Masyudhi Masyudhi Endang Sawitri Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro Journal of Tropical Pharmacy and Chemistry Eleutherine bulbosa Prophyromonas gingivalis Inhibition Zone Antibacterial Agents Periodontitis |
| title | Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro |
| title_full | Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro |
| title_fullStr | Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro |
| title_full_unstemmed | Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro |
| title_short | Effect of Ethanol Extract of Eleutherine bulbosa (Mill.) URB on Anaerobic Bacterial Prophyromonas gingivalis In Vitro |
| title_sort | effect of ethanol extract of eleutherine bulbosa mill urb on anaerobic bacterial prophyromonas gingivalis in vitro |
| topic | Eleutherine bulbosa Prophyromonas gingivalis Inhibition Zone Antibacterial Agents Periodontitis |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/151 |
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