Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw
The one-pot biomass conversion process is a promising strategy to minimize potential product loss and reduce processing costs. However, this strategy has technical limitations due to the inhibitory effects of biomass components like lignin as well as the generated inhibitors (e.g., furans, phenols)...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Chemical Engineering |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fceng.2022.1004240/full |
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| author | Daehwan Kim Anqi Ji Armoni L. Jackson Bailee Brown Youngmi Kim Sun Min Kim Craig Laufer Drew Ferrier Chang Geun Yoo |
| author_facet | Daehwan Kim Anqi Ji Armoni L. Jackson Bailee Brown Youngmi Kim Sun Min Kim Craig Laufer Drew Ferrier Chang Geun Yoo |
| author_sort | Daehwan Kim |
| collection | DOAJ |
| description | The one-pot biomass conversion process is a promising strategy to minimize potential product loss and reduce processing costs. However, this strategy has technical limitations due to the inhibitory effects of biomass components like lignin as well as the generated inhibitors (e.g., furans, phenols) during biomass processing. In this study, the inhibitory effects of liquid hydrolysates formed by hydrothermal pretreatment of soybean straw with either sodium hydroxide (NaOH) or hydrogen peroxide (H2O2) on cellulolytic enzyme activity were investigated. Hydrothermal pretreatment of soybean straw (10% w/v) was carried out with either sodium hydroxide (1% v/v) or hydrogen peroxide (1% v/v) at 121°C for 60 min to evaluate the effect of water-soluble inhibitors released from soybean pretreatment on cellulolytic enzyme activity. The fraction of cellulose in pretreated solids (1% w/v glucan) was enzymatically hydrolyzed for 72 h with 45 IU/g glucan (corresponding to 25 mg enzyme protein/g glucan) in the presence of either buffer or liquid hydrolysate generated from the pretreatments. Hydrolysis of NaOH and H2O2 pretreated solids resulted in 57% and 39% of glucose yields in buffer, respectively. In the presence of the liquid hydrolysates, NaOH and H2O2 pretreated biomass showed 20% and 30% glucose yield, respectively, indicating the enzyme suppression by inhibitors in the liquid hydrolysates. Of the enzyme activities in hydrolysates tested, NaOH hydrolysate showed a higher inhibitory effect on enzyme activities (mainly β-glucosidase) compared to H2O2 liquid, where enzyme deactivation has a first-order correlation and the manner in which the vacuum-filtered inhibitors were generated from pretreated soybean straw. |
| first_indexed | 2024-04-11T06:55:49Z |
| format | Article |
| id | doaj.art-7651a4f87c214b2582a6f1ba259c6b7c |
| institution | Directory Open Access Journal |
| issn | 2673-2718 |
| language | English |
| last_indexed | 2024-04-11T06:55:49Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemical Engineering |
| spelling | doaj.art-7651a4f87c214b2582a6f1ba259c6b7c2022-12-22T04:39:02ZengFrontiers Media S.A.Frontiers in Chemical Engineering2673-27182022-11-01410.3389/fceng.2022.10042401004240Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean strawDaehwan Kim0Anqi Ji1Armoni L. Jackson2Bailee Brown3Youngmi Kim4Sun Min Kim5Craig Laufer6Drew Ferrier7Chang Geun Yoo8Department of Biology, Hood College, Frederick, MD, United StatesDepartment of Chemical Engineering, State University of New York—College of Environmental Science and Forestry, Syracuse, NY, United StatesDepartment of Biology, Hood College, Frederick, MD, United StatesDepartment of Biology, Hood College, Frederick, MD, United StatesDepartment of Agricultural Engineering Technology, University of Wisconsin—River Falls, River Falls, WI, United StatesCargill Starches Sweeteners and Texturizers North America, Dayton, OH, United StatesDepartment of Biology, Hood College, Frederick, MD, United StatesDepartment of Biology, Hood College, Frederick, MD, United StatesDepartment of Chemical Engineering, State University of New York—College of Environmental Science and Forestry, Syracuse, NY, United StatesThe one-pot biomass conversion process is a promising strategy to minimize potential product loss and reduce processing costs. However, this strategy has technical limitations due to the inhibitory effects of biomass components like lignin as well as the generated inhibitors (e.g., furans, phenols) during biomass processing. In this study, the inhibitory effects of liquid hydrolysates formed by hydrothermal pretreatment of soybean straw with either sodium hydroxide (NaOH) or hydrogen peroxide (H2O2) on cellulolytic enzyme activity were investigated. Hydrothermal pretreatment of soybean straw (10% w/v) was carried out with either sodium hydroxide (1% v/v) or hydrogen peroxide (1% v/v) at 121°C for 60 min to evaluate the effect of water-soluble inhibitors released from soybean pretreatment on cellulolytic enzyme activity. The fraction of cellulose in pretreated solids (1% w/v glucan) was enzymatically hydrolyzed for 72 h with 45 IU/g glucan (corresponding to 25 mg enzyme protein/g glucan) in the presence of either buffer or liquid hydrolysate generated from the pretreatments. Hydrolysis of NaOH and H2O2 pretreated solids resulted in 57% and 39% of glucose yields in buffer, respectively. In the presence of the liquid hydrolysates, NaOH and H2O2 pretreated biomass showed 20% and 30% glucose yield, respectively, indicating the enzyme suppression by inhibitors in the liquid hydrolysates. Of the enzyme activities in hydrolysates tested, NaOH hydrolysate showed a higher inhibitory effect on enzyme activities (mainly β-glucosidase) compared to H2O2 liquid, where enzyme deactivation has a first-order correlation and the manner in which the vacuum-filtered inhibitors were generated from pretreated soybean straw.https://www.frontiersin.org/articles/10.3389/fceng.2022.1004240/fullsoybean strawpretreatmentenzyme hydrolysisinhibitionphenolic compounds |
| spellingShingle | Daehwan Kim Anqi Ji Armoni L. Jackson Bailee Brown Youngmi Kim Sun Min Kim Craig Laufer Drew Ferrier Chang Geun Yoo Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw Frontiers in Chemical Engineering soybean straw pretreatment enzyme hydrolysis inhibition phenolic compounds |
| title | Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw |
| title_full | Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw |
| title_fullStr | Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw |
| title_full_unstemmed | Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw |
| title_short | Inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw |
| title_sort | inhibition of cellulase activity by liquid hydrolysates from hydrothermally pretreated soybean straw |
| topic | soybean straw pretreatment enzyme hydrolysis inhibition phenolic compounds |
| url | https://www.frontiersin.org/articles/10.3389/fceng.2022.1004240/full |
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