Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro
The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissocia...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Brain Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3425/11/6/717 |
| _version_ | 1797532327781335040 |
|---|---|
| author | Yana Pigareva Arseniy Gladkov Vladimir Kolpakov Irina Mukhina Anton Bukatin Victor B. Kazantsev Alexey Pimashkin |
| author_facet | Yana Pigareva Arseniy Gladkov Vladimir Kolpakov Irina Mukhina Anton Bukatin Victor B. Kazantsev Alexey Pimashkin |
| author_sort | Yana Pigareva |
| collection | DOAJ |
| description | The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissociated cells with unidirectional synaptic connections formed by asymmetric microchannels. The complexity of the microchannel geometry defined the strength of the synaptic connectivity and the properties of spiking activity propagation. In this study, we developed an experimental platform to study the effects of synaptic plasticity on a network level with predefined locations of unidirectionally connected cellular assemblies using multisite extracellular electrophysiology. |
| first_indexed | 2024-03-10T10:57:34Z |
| format | Article |
| id | doaj.art-7652b575af344d88b15e39ffe7ddb7c3 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-03-10T10:57:34Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj.art-7652b575af344d88b15e39ffe7ddb7c32023-11-21T21:46:55ZengMDPI AGBrain Sciences2076-34252021-05-0111671710.3390/brainsci11060717Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In VitroYana Pigareva0Arseniy Gladkov1Vladimir Kolpakov2Irina Mukhina3Anton Bukatin4Victor B. Kazantsev5Alexey Pimashkin6Neurotechnology Department, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaNeurotechnology Department, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaNeurotechnology Department, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaNeurotechnology Department, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaThe Laboratory of Renewable Energy Sources, Alferov Saint-Petersburg National Research Academic University of the Russian Academy of Sciences, 194021 Saint-Petersburg, RussiaNeurotechnology Department, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaNeurotechnology Department, Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaThe structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissociated cells with unidirectional synaptic connections formed by asymmetric microchannels. The complexity of the microchannel geometry defined the strength of the synaptic connectivity and the properties of spiking activity propagation. In this study, we developed an experimental platform to study the effects of synaptic plasticity on a network level with predefined locations of unidirectionally connected cellular assemblies using multisite extracellular electrophysiology.https://www.mdpi.com/2076-3425/11/6/717microfluidicsdissociated culturemodular neural networksmicroelectrode arraysynaptic plasticity |
| spellingShingle | Yana Pigareva Arseniy Gladkov Vladimir Kolpakov Irina Mukhina Anton Bukatin Victor B. Kazantsev Alexey Pimashkin Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro Brain Sciences microfluidics dissociated culture modular neural networks microelectrode array synaptic plasticity |
| title | Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro |
| title_full | Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro |
| title_fullStr | Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro |
| title_full_unstemmed | Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro |
| title_short | Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro |
| title_sort | experimental platform to study spiking pattern propagation in modular networks in vitro |
| topic | microfluidics dissociated culture modular neural networks microelectrode array synaptic plasticity |
| url | https://www.mdpi.com/2076-3425/11/6/717 |
| work_keys_str_mv | AT yanapigareva experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro AT arseniygladkov experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro AT vladimirkolpakov experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro AT irinamukhina experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro AT antonbukatin experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro AT victorbkazantsev experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro AT alexeypimashkin experimentalplatformtostudyspikingpatternpropagationinmodularnetworksinvitro |