Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

Cloning, expression, and one-step purification/immobilization of two carbohydrate-binding module-tagged alcohol dehydrogenases

Abstract Background The feasibility of biochemical transformation processes is usually greatly dependent on biocatalysts cost. Therefore, immobilizing and reusing biocatalysts is an approach to be considered to bring biotransformations closer to industrial feasibility, since it does not only allow t...

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Bibliographic Details
Main Authors: Mario Benito, Ramón Román, Garazi Ortiz, Antoni Casablancas, Gregorio Álvaro, Gloria Caminal, Gloria González, Marina Guillén
Format: Article
Language:English
Published: BMC 2022-06-01
Series:Journal of Biological Engineering
Subjects:
Carbohydrate-binding module
Escherichia coli
Alcohol dehydrogenase
One-step immobilization/purification
Regenerated amorphous cellulose
Online Access:https://doi.org/10.1186/s13036-022-00295-8

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