CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i>
As a common abiotic stress, drought severely impairs the growth, development, and even survival of plants. Here we report a transcription factor, <i>Caragana korshinskii</i> REVOLUTA(CkREV), which can bidirectionally regulate the expression of the critical enzyme gene <i>CkYUC5<...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/11/5902 |
| _version_ | 1797493307369062400 |
|---|---|
| author | Jia-Yang Li Jie-Jie Ren Tian-Xin Zhang Jin-Hao Cui Chun-Mei Gong |
| author_facet | Jia-Yang Li Jie-Jie Ren Tian-Xin Zhang Jin-Hao Cui Chun-Mei Gong |
| author_sort | Jia-Yang Li |
| collection | DOAJ |
| description | As a common abiotic stress, drought severely impairs the growth, development, and even survival of plants. Here we report a transcription factor, <i>Caragana korshinskii</i> REVOLUTA(CkREV), which can bidirectionally regulate the expression of the critical enzyme gene <i>CkYUC5</i> in auxin synthesis according to external environment changes, so as to control the biosynthesis of auxin and further enhance the drought resistance of plants. Quantitative analysis reveals that the expression level of both <i>CkYUC5</i> and <i>AtYUC5</i> is down-regulated after <i>C. korshinskii</i> and <i>Arabidopsis thaliana</i> are exposed to drought. Functional verification of CkREV reveals that CkREV up-regulates the expression of <i>AtYUC5</i> in transgenic <i>A. thaliana</i> under common conditions, while down-regulating it under drought conditions. Meanwhile, the expression of <i>CkYUC5</i> is also down-regulated in <i>C. korshinskii</i> leaves instantaneously overexpressing CkREV. We apply a dual-luciferase reporter system to discover that CkREV can bind to the promoter of <i>CkYUC5</i> to regulate its expression, which is further proved by EMSA and Y1H esxperiments. Functional verification of CkREV in <i>C. korshinskii</i> and transgenic <i>A. thaliana</i> shows that CkREV can regulate the expression of <i>CkYUC5</i> and <i>AtYUC5</i> in a contrary way, maintaining the equilibrium of plants between growth and drought resisting. CkREV can positively regulate the expression of <i>CkYUC5</i> to promote auxin synthesis in favor of growth under normal development. However, CkREV can also respond to external signals and negatively regulate the expression of <i>CkYUC5</i>, which inhibits auxin synthesis in order to reduce growth rate, lower water demands, and eventually improve the drought resistance of plants. |
| first_indexed | 2024-03-10T01:18:08Z |
| format | Article |
| id | doaj.art-76542c313a2f40be8e7c1652149c5b50 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:18:08Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-76542c313a2f40be8e7c1652149c5b502023-11-23T14:06:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311590210.3390/ijms23115902CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i>Jia-Yang Li0Jie-Jie Ren1Tian-Xin Zhang2Jin-Hao Cui3Chun-Mei Gong4College of Horticulture, Northwest A&F University, Xianyang 712100, ChinaCollege of Horticulture, Northwest A&F University, Xianyang 712100, ChinaCollege of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaCollege of Life Sciences, Northwest A&F University, Xianyang 712100, ChinaCollege of Horticulture, Northwest A&F University, Xianyang 712100, ChinaAs a common abiotic stress, drought severely impairs the growth, development, and even survival of plants. Here we report a transcription factor, <i>Caragana korshinskii</i> REVOLUTA(CkREV), which can bidirectionally regulate the expression of the critical enzyme gene <i>CkYUC5</i> in auxin synthesis according to external environment changes, so as to control the biosynthesis of auxin and further enhance the drought resistance of plants. Quantitative analysis reveals that the expression level of both <i>CkYUC5</i> and <i>AtYUC5</i> is down-regulated after <i>C. korshinskii</i> and <i>Arabidopsis thaliana</i> are exposed to drought. Functional verification of CkREV reveals that CkREV up-regulates the expression of <i>AtYUC5</i> in transgenic <i>A. thaliana</i> under common conditions, while down-regulating it under drought conditions. Meanwhile, the expression of <i>CkYUC5</i> is also down-regulated in <i>C. korshinskii</i> leaves instantaneously overexpressing CkREV. We apply a dual-luciferase reporter system to discover that CkREV can bind to the promoter of <i>CkYUC5</i> to regulate its expression, which is further proved by EMSA and Y1H esxperiments. Functional verification of CkREV in <i>C. korshinskii</i> and transgenic <i>A. thaliana</i> shows that CkREV can regulate the expression of <i>CkYUC5</i> and <i>AtYUC5</i> in a contrary way, maintaining the equilibrium of plants between growth and drought resisting. CkREV can positively regulate the expression of <i>CkYUC5</i> to promote auxin synthesis in favor of growth under normal development. However, CkREV can also respond to external signals and negatively regulate the expression of <i>CkYUC5</i>, which inhibits auxin synthesis in order to reduce growth rate, lower water demands, and eventually improve the drought resistance of plants.https://www.mdpi.com/1422-0067/23/11/5902<i>Caragana korshinskii</i>droughtauxin synthesisHD-ZIP IIIstress resistance |
| spellingShingle | Jia-Yang Li Jie-Jie Ren Tian-Xin Zhang Jin-Hao Cui Chun-Mei Gong CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i> International Journal of Molecular Sciences <i>Caragana korshinskii</i> drought auxin synthesis HD-ZIP III stress resistance |
| title | CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i> |
| title_full | CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i> |
| title_fullStr | CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i> |
| title_full_unstemmed | CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i> |
| title_short | CkREV Enhances the Drought Resistance of <i>Caragana korshinskii</i> through Regulating the Expression of Auxin Synthetase Gene <i>CkYUC5</i> |
| title_sort | ckrev enhances the drought resistance of i caragana korshinskii i through regulating the expression of auxin synthetase gene i ckyuc5 i |
| topic | <i>Caragana korshinskii</i> drought auxin synthesis HD-ZIP III stress resistance |
| url | https://www.mdpi.com/1422-0067/23/11/5902 |
| work_keys_str_mv | AT jiayangli ckrevenhancesthedroughtresistanceoficaraganakorshinskiiithroughregulatingtheexpressionofauxinsynthetasegeneickyuc5i AT jiejieren ckrevenhancesthedroughtresistanceoficaraganakorshinskiiithroughregulatingtheexpressionofauxinsynthetasegeneickyuc5i AT tianxinzhang ckrevenhancesthedroughtresistanceoficaraganakorshinskiiithroughregulatingtheexpressionofauxinsynthetasegeneickyuc5i AT jinhaocui ckrevenhancesthedroughtresistanceoficaraganakorshinskiiithroughregulatingtheexpressionofauxinsynthetasegeneickyuc5i AT chunmeigong ckrevenhancesthedroughtresistanceoficaraganakorshinskiiithroughregulatingtheexpressionofauxinsynthetasegeneickyuc5i |