Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche
Abstract Background Skeletal muscle regeneration is possible thanks to unipotent stem cells, which are satellite cells connected to the myofibers. Populations of stem cells other than muscle-specific satellite cells are considered as sources of cells able to support skeletal muscle reconstruction. A...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-09-01
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| Series: | Stem Cell Research & Therapy |
| Online Access: | http://link.springer.com/article/10.1186/s13287-018-0993-z |
| _version_ | 1819054085211422720 |
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| author | Kamil Kowalski Matthieu Dos Santos Pascal Maire Maria A. Ciemerych Edyta Brzoska |
| author_facet | Kamil Kowalski Matthieu Dos Santos Pascal Maire Maria A. Ciemerych Edyta Brzoska |
| author_sort | Kamil Kowalski |
| collection | DOAJ |
| description | Abstract Background Skeletal muscle regeneration is possible thanks to unipotent stem cells, which are satellite cells connected to the myofibers. Populations of stem cells other than muscle-specific satellite cells are considered as sources of cells able to support skeletal muscle reconstruction. Among these are bone marrow-derived mesenchymal stem cells (BM-MSCs), which are multipotent, self-renewing stem cells present in the bone marrow stroma. Available data documenting the ability of BM-MSCs to undergo myogenic differentiation are not definitive. In the current work, we aimed to check if the satellite cell niche could impact the ability of bone marrow-derived cells to follow a myogenic program. Methods We established a new in-vitro method for the coculture of bone marrow-derived cells (BMCs) that express CXCR4 (CXCR4+BMCs; the stromal-derived factor-1 (Sdf-1) receptor) with myofibers. Using various tests, we analyzed the myogenic identity of BMCs and their ability to fuse with myoblasts in vitro and in vivo. Results We showed that Sdf-1 treatment increased the number of CXCR4+BMCs able to bind the myofiber and occupy the satellite cell niche. Moreover, interaction with myofibers induced the expression of myogenic regulatory factors (MRFs) in CXCR4+BMCs. CXCR4+BMCs, pretreated by the coculture with myofibers and Sdf-1, participated in myotube formation in vitro and also myofiber reconstruction in vivo. We also showed that Sdf-1 overexpression in vivo (in injured and regenerating muscles) supported the participation of CXCR4+BMCs in new myofiber formation. Conclusion We showed that CXCR4+BMC interaction with myofibers (that is, within the satellite cell niche) induced CXCR4+BMC myogenic commitment. CXCR4+BMCs, pretreated using such a method of culture, were able to participate in skeletal muscle regeneration. |
| first_indexed | 2024-12-21T12:46:01Z |
| format | Article |
| id | doaj.art-765504c67f73453ea6e3c3f685035297 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-21T12:46:01Z |
| publishDate | 2018-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-765504c67f73453ea6e3c3f6850352972022-12-21T19:03:38ZengBMCStem Cell Research & Therapy1757-65122018-09-019111410.1186/s13287-018-0993-zInduction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell nicheKamil Kowalski0Matthieu Dos Santos1Pascal Maire2Maria A. Ciemerych3Edyta Brzoska4Department of Cytology, Faculty of Biology, University of WarsawInstitut Cochin, Université Paris-Descartes, Centre National de la Recherche Scientifique (CNRS), UMR 8104Institut Cochin, Université Paris-Descartes, Centre National de la Recherche Scientifique (CNRS), UMR 8104Department of Cytology, Faculty of Biology, University of WarsawDepartment of Cytology, Faculty of Biology, University of WarsawAbstract Background Skeletal muscle regeneration is possible thanks to unipotent stem cells, which are satellite cells connected to the myofibers. Populations of stem cells other than muscle-specific satellite cells are considered as sources of cells able to support skeletal muscle reconstruction. Among these are bone marrow-derived mesenchymal stem cells (BM-MSCs), which are multipotent, self-renewing stem cells present in the bone marrow stroma. Available data documenting the ability of BM-MSCs to undergo myogenic differentiation are not definitive. In the current work, we aimed to check if the satellite cell niche could impact the ability of bone marrow-derived cells to follow a myogenic program. Methods We established a new in-vitro method for the coculture of bone marrow-derived cells (BMCs) that express CXCR4 (CXCR4+BMCs; the stromal-derived factor-1 (Sdf-1) receptor) with myofibers. Using various tests, we analyzed the myogenic identity of BMCs and their ability to fuse with myoblasts in vitro and in vivo. Results We showed that Sdf-1 treatment increased the number of CXCR4+BMCs able to bind the myofiber and occupy the satellite cell niche. Moreover, interaction with myofibers induced the expression of myogenic regulatory factors (MRFs) in CXCR4+BMCs. CXCR4+BMCs, pretreated by the coculture with myofibers and Sdf-1, participated in myotube formation in vitro and also myofiber reconstruction in vivo. We also showed that Sdf-1 overexpression in vivo (in injured and regenerating muscles) supported the participation of CXCR4+BMCs in new myofiber formation. Conclusion We showed that CXCR4+BMC interaction with myofibers (that is, within the satellite cell niche) induced CXCR4+BMC myogenic commitment. CXCR4+BMCs, pretreated using such a method of culture, were able to participate in skeletal muscle regeneration.http://link.springer.com/article/10.1186/s13287-018-0993-z |
| spellingShingle | Kamil Kowalski Matthieu Dos Santos Pascal Maire Maria A. Ciemerych Edyta Brzoska Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche Stem Cell Research & Therapy |
| title | Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche |
| title_full | Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche |
| title_fullStr | Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche |
| title_full_unstemmed | Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche |
| title_short | Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche |
| title_sort | induction of bone marrow derived cells myogenic identity by their interactions with the satellite cell niche |
| url | http://link.springer.com/article/10.1186/s13287-018-0993-z |
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