13C MRI of hyperpolarized pyruvate at 120 µT
Abstract Nuclear spin hyperpolarization increases the sensitivity of magnetic resonance dramatically, enabling many new applications, including real-time metabolic imaging. Parahydrogen-based signal amplification by reversible exchange (SABRE) was employed to hyperpolarize [1-13C]pyruvate and demons...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-54770-x |
| _version_ | 1797275213287653376 |
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| author | Nicolas Kempf Rainer Körber Markus Plaumann Andrey N. Pravdivtsev Jörn Engelmann Johannes Boldt Klaus Scheffler Thomas Theis Kai Buckenmaier |
| author_facet | Nicolas Kempf Rainer Körber Markus Plaumann Andrey N. Pravdivtsev Jörn Engelmann Johannes Boldt Klaus Scheffler Thomas Theis Kai Buckenmaier |
| author_sort | Nicolas Kempf |
| collection | DOAJ |
| description | Abstract Nuclear spin hyperpolarization increases the sensitivity of magnetic resonance dramatically, enabling many new applications, including real-time metabolic imaging. Parahydrogen-based signal amplification by reversible exchange (SABRE) was employed to hyperpolarize [1-13C]pyruvate and demonstrate 13C imaging in situ at 120 µT, about twice Earth’s magnetic field, with two different signal amplification by reversible exchange variants: SABRE in shield enables alignment transfer to heteronuclei (SABRE-SHEATH), where hyperpolarization is transferred from parahydrogen to [1-13C]pyruvate at a magnetic field below 1 µT, and low-irradiation generates high tesla (LIGHT-SABRE), where hyperpolarization was prepared at 120 µT, avoiding magnetic field cycling. The 3-dimensional images of a phantom were obtained using a superconducting quantum interference device (SQUID) based magnetic field detector with submillimeter resolution. These 13C images demonstrate the feasibility of low-field 13C metabolic magnetic resonance imaging (MRI) of 50 mM [1-13C]pyruvate hyperpolarized by parahydrogen in reversible exchange imaged at about twice Earth’s magnetic field. Using thermal 13C polarization available at 120 µT, the same experiment would have taken about 300 billion years. |
| first_indexed | 2024-03-07T15:10:09Z |
| format | Article |
| id | doaj.art-7655cbf0626440f38c811e53fb01f8b7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:10:09Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7655cbf0626440f38c811e53fb01f8b72024-03-05T18:40:34ZengNature PortfolioScientific Reports2045-23222024-02-011411710.1038/s41598-024-54770-x13C MRI of hyperpolarized pyruvate at 120 µTNicolas Kempf0Rainer Körber1Markus Plaumann2Andrey N. Pravdivtsev3Jörn Engelmann4Johannes Boldt5Klaus Scheffler6Thomas Theis7Kai Buckenmaier8High-Field Magnetic Resonance Center, Max Planck Institute for Biological CyberneticsPhysikalisch-Technische BundesanstaltInstitute for Molecular Biology and Medicinal Chemistry, Medical Faculty, Otto-von-Guericke UniversitySection Biomedical Imaging, Molecular Imaging North Competence Center (MOIN CC), Department of Radiology and Neuroradiology, University Medical Center, Kiel UniversityHigh-Field Magnetic Resonance Center, Max Planck Institute for Biological CyberneticsHigh-Field Magnetic Resonance Center, Max Planck Institute for Biological CyberneticsHigh-Field Magnetic Resonance Center, Max Planck Institute for Biological CyberneticsHigh-Field Magnetic Resonance Center, Max Planck Institute for Biological CyberneticsHigh-Field Magnetic Resonance Center, Max Planck Institute for Biological CyberneticsAbstract Nuclear spin hyperpolarization increases the sensitivity of magnetic resonance dramatically, enabling many new applications, including real-time metabolic imaging. Parahydrogen-based signal amplification by reversible exchange (SABRE) was employed to hyperpolarize [1-13C]pyruvate and demonstrate 13C imaging in situ at 120 µT, about twice Earth’s magnetic field, with two different signal amplification by reversible exchange variants: SABRE in shield enables alignment transfer to heteronuclei (SABRE-SHEATH), where hyperpolarization is transferred from parahydrogen to [1-13C]pyruvate at a magnetic field below 1 µT, and low-irradiation generates high tesla (LIGHT-SABRE), where hyperpolarization was prepared at 120 µT, avoiding magnetic field cycling. The 3-dimensional images of a phantom were obtained using a superconducting quantum interference device (SQUID) based magnetic field detector with submillimeter resolution. These 13C images demonstrate the feasibility of low-field 13C metabolic magnetic resonance imaging (MRI) of 50 mM [1-13C]pyruvate hyperpolarized by parahydrogen in reversible exchange imaged at about twice Earth’s magnetic field. Using thermal 13C polarization available at 120 µT, the same experiment would have taken about 300 billion years.https://doi.org/10.1038/s41598-024-54770-x |
| spellingShingle | Nicolas Kempf Rainer Körber Markus Plaumann Andrey N. Pravdivtsev Jörn Engelmann Johannes Boldt Klaus Scheffler Thomas Theis Kai Buckenmaier 13C MRI of hyperpolarized pyruvate at 120 µT Scientific Reports |
| title | 13C MRI of hyperpolarized pyruvate at 120 µT |
| title_full | 13C MRI of hyperpolarized pyruvate at 120 µT |
| title_fullStr | 13C MRI of hyperpolarized pyruvate at 120 µT |
| title_full_unstemmed | 13C MRI of hyperpolarized pyruvate at 120 µT |
| title_short | 13C MRI of hyperpolarized pyruvate at 120 µT |
| title_sort | 13c mri of hyperpolarized pyruvate at 120 µt |
| url | https://doi.org/10.1038/s41598-024-54770-x |
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