Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau
In the extreme alpine climate of the Qinghai-Tibet Plateau (QTP), plant growth and reproduction are limited by extremely cold temperatures, low soil moisture, and scarce nutrient availability. The root-associated microbiome indirectly promotes plant growth and plays a role in the fitness of plants o...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2023-05-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/15361.pdf |
| _version_ | 1827610775529717760 |
|---|---|
| author | Shuting Chen Pengxi Cao Ting Li Yuyan Wang Xing Liu |
| author_facet | Shuting Chen Pengxi Cao Ting Li Yuyan Wang Xing Liu |
| author_sort | Shuting Chen |
| collection | DOAJ |
| description | In the extreme alpine climate of the Qinghai-Tibet Plateau (QTP), plant growth and reproduction are limited by extremely cold temperatures, low soil moisture, and scarce nutrient availability. The root-associated microbiome indirectly promotes plant growth and plays a role in the fitness of plants on the QTP, particularly in Tibetan medicinal plants. Despite the importance of the root-associated microbiome, little is known about the root zone. This study used high-throughput sequencing to investigate two medicinal Meconopsis plants, M. horridula and M. integrifolia, to determine whether habitat or plant identity had a more significant impact on the microbial composition of the roots. The fungal sequences were obtained using ITS-1 and ITS-2, and bacterial sequences were obtained using 16S rRNA. Different microbial patterns were observed in the microbial compositions of fungi and bacteria in the root zones of two Meconopsis plants. In contrast to bacteria, which were not significantly impacted by plant identity or habitat, the fungi in the root zone were significantly impacted by plant identity, but not habitat. In addition, the synergistic effect was more significant than the antagonistic effect in the correlation between fungi and bacteria in the root zone soil. The fungal structure was influenced by total nitrogen and pH, whereas the structure of bacterial communities was influenced by soil moisture and organic matter. Plant identity had a greater influence on fungal structure than habitat in two Meconopsis plants. The dissimilarity of fungal communities suggests that more attention should be paid to fungi-plant interactions. |
| first_indexed | 2024-03-09T07:55:41Z |
| format | Article |
| id | doaj.art-765669dce7144588805f52bf177b4ce9 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:55:41Z |
| publishDate | 2023-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-765669dce7144588805f52bf177b4ce92023-12-03T01:01:10ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1536110.7717/peerj.15361Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet PlateauShuting Chen0Pengxi Cao1Ting Li2Yuyan Wang3Xing Liu4Laboratory of Adaptation and Evolution of Plateau Biota to Extreme Environments, School of Ecology and Environment, Tibet University, Lhasa, ChinaLaboratory of Adaptation and Evolution of Plateau Biota to Extreme Environments, School of Ecology and Environment, Tibet University, Lhasa, ChinaLaboratory of Adaptation and Evolution of Plateau Biota to Extreme Environments, School of Ecology and Environment, Tibet University, Lhasa, ChinaLaboratory of Adaptation and Evolution of Plateau Biota to Extreme Environments, School of Ecology and Environment, Tibet University, Lhasa, ChinaLaboratory of Adaptation and Evolution of Plateau Biota to Extreme Environments, School of Ecology and Environment, Tibet University, Lhasa, ChinaIn the extreme alpine climate of the Qinghai-Tibet Plateau (QTP), plant growth and reproduction are limited by extremely cold temperatures, low soil moisture, and scarce nutrient availability. The root-associated microbiome indirectly promotes plant growth and plays a role in the fitness of plants on the QTP, particularly in Tibetan medicinal plants. Despite the importance of the root-associated microbiome, little is known about the root zone. This study used high-throughput sequencing to investigate two medicinal Meconopsis plants, M. horridula and M. integrifolia, to determine whether habitat or plant identity had a more significant impact on the microbial composition of the roots. The fungal sequences were obtained using ITS-1 and ITS-2, and bacterial sequences were obtained using 16S rRNA. Different microbial patterns were observed in the microbial compositions of fungi and bacteria in the root zones of two Meconopsis plants. In contrast to bacteria, which were not significantly impacted by plant identity or habitat, the fungi in the root zone were significantly impacted by plant identity, but not habitat. In addition, the synergistic effect was more significant than the antagonistic effect in the correlation between fungi and bacteria in the root zone soil. The fungal structure was influenced by total nitrogen and pH, whereas the structure of bacterial communities was influenced by soil moisture and organic matter. Plant identity had a greater influence on fungal structure than habitat in two Meconopsis plants. The dissimilarity of fungal communities suggests that more attention should be paid to fungi-plant interactions.https://peerj.com/articles/15361.pdfMicrobial communityBacterial communitiesFungal communitiesSoil propertiesCommunity ecologyMicrobial structure |
| spellingShingle | Shuting Chen Pengxi Cao Ting Li Yuyan Wang Xing Liu Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau PeerJ Microbial community Bacterial communities Fungal communities Soil properties Community ecology Microbial structure |
| title | Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau |
| title_full | Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau |
| title_fullStr | Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau |
| title_full_unstemmed | Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau |
| title_short | Microbial diversity patterns in the root zone of two Meconopsis plants on the Qinghai-Tibet Plateau |
| title_sort | microbial diversity patterns in the root zone of two meconopsis plants on the qinghai tibet plateau |
| topic | Microbial community Bacterial communities Fungal communities Soil properties Community ecology Microbial structure |
| url | https://peerj.com/articles/15361.pdf |
| work_keys_str_mv | AT shutingchen microbialdiversitypatternsintherootzoneoftwomeconopsisplantsontheqinghaitibetplateau AT pengxicao microbialdiversitypatternsintherootzoneoftwomeconopsisplantsontheqinghaitibetplateau AT tingli microbialdiversitypatternsintherootzoneoftwomeconopsisplantsontheqinghaitibetplateau AT yuyanwang microbialdiversitypatternsintherootzoneoftwomeconopsisplantsontheqinghaitibetplateau AT xingliu microbialdiversitypatternsintherootzoneoftwomeconopsisplantsontheqinghaitibetplateau |