The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome
Chemerin is an adipocyte derived signalling molecule (adipokine) that serves as a ligand activator of Chemokine-like receptor 1(CMKLR1). Chemerin/CMKLR1 signalling is well established to regulate fundamental processes in metabolism and inflammation. The composition and function of gut microbiota has...
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PeerJ Inc.
2018-09-01
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| Online Access: | https://peerj.com/articles/5494.pdf |
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| author | Helen J. Dranse Ashlee Zheng André M. Comeau Morgan G.I. Langille Brian A. Zabel Christopher J. Sinal |
| author_facet | Helen J. Dranse Ashlee Zheng André M. Comeau Morgan G.I. Langille Brian A. Zabel Christopher J. Sinal |
| author_sort | Helen J. Dranse |
| collection | DOAJ |
| description | Chemerin is an adipocyte derived signalling molecule (adipokine) that serves as a ligand activator of Chemokine-like receptor 1(CMKLR1). Chemerin/CMKLR1 signalling is well established to regulate fundamental processes in metabolism and inflammation. The composition and function of gut microbiota has also been shown to impact the development of metabolic and inflammatory diseases such as obesity, diabetes and inflammatory bowel disease. In this study, we assessed the microbiome composition of fecal samples isolated from wildtype, chemerin, or CMKLR1 knockout mice using Illumina-based sequencing. Moreover, the knockout mice and respective wildtype mice used in this study were housed at different universities allowing us to compare facility-dependent effects on microbiome composition. While there was no difference in alpha diversity within samples when compared by either facility or genotype, we observed a dramatic difference in the presence and abundance of numerous taxa between facilities. There were minor differences in bacterial abundance between wildtype and chemerin knockout mice, but significantly more differences in taxa abundance between wildtype and CMKLR1 knockout mice. Specifically, CMKLR1 knockout mice exhibited decreased abundance of Akkermansia and Prevotella, which correlated with body weight in CMKLR1 knockout, but not wildtype mice. This is the first study to investigate a linkage between chemerin/CMKLR1 signaling and microbiome composition. The results of our study suggest that chemerin/CMKLR1 signaling influences metabolic processes through effects on the gut microbiome. Furthermore, the dramatic difference in microbiome composition between facilities might contribute to discrepancies in the metabolic phenotype of CMKLR1 knockout mice reported by independent groups. Considered altogether, these findings establish a foundation for future studies to investigate the relationship between chemerin signaling and the gut microbiome on the development and progression of metabolic and inflammatory disease. |
| first_indexed | 2024-03-09T06:45:20Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:45:20Z |
| publishDate | 2018-09-01 |
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| spelling | doaj.art-7659fa36726e4a4d8c35a0d5e36a0f8c2023-12-03T10:37:47ZengPeerJ Inc.PeerJ2167-83592018-09-016e549410.7717/peerj.5494The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiomeHelen J. Dranse0Ashlee Zheng1André M. Comeau2Morgan G.I. Langille3Brian A. Zabel4Christopher J. Sinal5Department of Pharmacology, Dalhousie University, Halifax, CanadaPalo Alto Institute for Research and Education, Veterans Affairs Palo Alto Health Care System, Palo Alto, United States of AmericaDepartment of Pharmacology, Dalhousie University, Halifax, CanadaDepartment of Pharmacology, Dalhousie University, Halifax, CanadaPalo Alto Institute for Research and Education, Veterans Affairs Palo Alto Health Care System, Palo Alto, United States of AmericaDepartment of Pharmacology, Dalhousie University, Halifax, CanadaChemerin is an adipocyte derived signalling molecule (adipokine) that serves as a ligand activator of Chemokine-like receptor 1(CMKLR1). Chemerin/CMKLR1 signalling is well established to regulate fundamental processes in metabolism and inflammation. The composition and function of gut microbiota has also been shown to impact the development of metabolic and inflammatory diseases such as obesity, diabetes and inflammatory bowel disease. In this study, we assessed the microbiome composition of fecal samples isolated from wildtype, chemerin, or CMKLR1 knockout mice using Illumina-based sequencing. Moreover, the knockout mice and respective wildtype mice used in this study were housed at different universities allowing us to compare facility-dependent effects on microbiome composition. While there was no difference in alpha diversity within samples when compared by either facility or genotype, we observed a dramatic difference in the presence and abundance of numerous taxa between facilities. There were minor differences in bacterial abundance between wildtype and chemerin knockout mice, but significantly more differences in taxa abundance between wildtype and CMKLR1 knockout mice. Specifically, CMKLR1 knockout mice exhibited decreased abundance of Akkermansia and Prevotella, which correlated with body weight in CMKLR1 knockout, but not wildtype mice. This is the first study to investigate a linkage between chemerin/CMKLR1 signaling and microbiome composition. The results of our study suggest that chemerin/CMKLR1 signaling influences metabolic processes through effects on the gut microbiome. Furthermore, the dramatic difference in microbiome composition between facilities might contribute to discrepancies in the metabolic phenotype of CMKLR1 knockout mice reported by independent groups. Considered altogether, these findings establish a foundation for future studies to investigate the relationship between chemerin signaling and the gut microbiome on the development and progression of metabolic and inflammatory disease.https://peerj.com/articles/5494.pdfMicrobiomeChemerinChemokine-like receptor 1AdipokineMouseAdipose |
| spellingShingle | Helen J. Dranse Ashlee Zheng André M. Comeau Morgan G.I. Langille Brian A. Zabel Christopher J. Sinal The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome PeerJ Microbiome Chemerin Chemokine-like receptor 1 Adipokine Mouse Adipose |
| title | The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome |
| title_full | The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome |
| title_fullStr | The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome |
| title_full_unstemmed | The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome |
| title_short | The impact of chemerin or chemokine-like receptor 1 loss on the mouse gut microbiome |
| title_sort | impact of chemerin or chemokine like receptor 1 loss on the mouse gut microbiome |
| topic | Microbiome Chemerin Chemokine-like receptor 1 Adipokine Mouse Adipose |
| url | https://peerj.com/articles/5494.pdf |
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