Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein
Abstract Liver ischemia–reperfusion (IR) injury is associated with poor outcome after liver transplantation and liver resections. Hexafluoroisopropanol (HFIP) is a tri‐fluorinated metabolites of volatile anesthetics and has modulatory effects on inflammation that have been observed mainly in cell cu...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Pharmacology Research & Perspectives |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/prp2.1027 |
| _version_ | 1798005764937220096 |
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| author | Agustin Vintimilla Moscoso Estela Regina Ramos Figueira Joel Avancini Rocha‐Filho Martin Urner Cinthia Lanchotte Jose Jukemura Jorge Luiz Saraiva Ximenes Sergio Carlos Nahas Luiz Augusto Carneiro D'Albuquerque Flavio Henrique Ferreira Galvao |
| author_facet | Agustin Vintimilla Moscoso Estela Regina Ramos Figueira Joel Avancini Rocha‐Filho Martin Urner Cinthia Lanchotte Jose Jukemura Jorge Luiz Saraiva Ximenes Sergio Carlos Nahas Luiz Augusto Carneiro D'Albuquerque Flavio Henrique Ferreira Galvao |
| author_sort | Agustin Vintimilla Moscoso |
| collection | DOAJ |
| description | Abstract Liver ischemia–reperfusion (IR) injury is associated with poor outcome after liver transplantation and liver resections. Hexafluoroisopropanol (HFIP) is a tri‐fluorinated metabolites of volatile anesthetics and has modulatory effects on inflammation that have been observed mainly in cell culture experiments. In this survey, we investigated the effects of HFIP in a rat model of normothermic hepatic ischemia–reperfusion injury. Twenty‐four male Wistar rats were randomized into three groups: (1) control in which animals were submitted to 30 min of partial liver ischemia with resection of non‐ischemic liver lobes immediate after reperfusion, (2) pre‐ischemia (PI) group in which animals received intravenous HFIP (67 mg/kg) 5 min before liver ischemia, and (3) pre‐reperfusion (PR) group in which animals received intravenous HFIP (67 mg/kg) 5 min before reperfusion. Four hours after reperfusion, all animals were euthanized for sample collection. Aspartate and alanine transaminases, glucose, and high mobility group box‐1 (HMGB‐1) protein concentrations showed a significant decreased, and malondialdehyde was increased in the PR group compared with control and PI groups. Interleukin 6 (IL‐6) was increased in the PI group compared with control and PR groups. IL‐10 and ‐12 were increased in the PR and PI groups, respectively, when compared with the control group. Glucose decreased in the PR when compared with the control group. Post‐conditioning with HFIP led to a decrease in hepatocellular injury and was associated with a downregulation of HMGB‐1. The HFIP resulted in a better control of inflammatory response to ischemia–reperfusion even without causing a reduction in oxidative stress. |
| first_indexed | 2024-04-11T12:44:18Z |
| format | Article |
| id | doaj.art-765ca6ecfcb64b28a2bfd749ceab97ee |
| institution | Directory Open Access Journal |
| issn | 2052-1707 |
| language | English |
| last_indexed | 2024-04-11T12:44:18Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pharmacology Research & Perspectives |
| spelling | doaj.art-765ca6ecfcb64b28a2bfd749ceab97ee2022-12-22T04:23:24ZengWileyPharmacology Research & Perspectives2052-17072022-12-01106n/an/a10.1002/prp2.1027Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 proteinAgustin Vintimilla Moscoso0Estela Regina Ramos Figueira1Joel Avancini Rocha‐Filho2Martin Urner3Cinthia Lanchotte4Jose Jukemura5Jorge Luiz Saraiva Ximenes6Sergio Carlos Nahas7Luiz Augusto Carneiro D'Albuquerque8Flavio Henrique Ferreira Galvao9Laboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilDivisao Interdepartamental de Medicina Intensiva Universidade de Toronto Toronto Ontario CanadaLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilDepartamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilLaboratorio de Investigaçao Medica 37, Departamento de Gastroenterologia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina Universidade de Sao Paulo Sao Paulo BrazilAbstract Liver ischemia–reperfusion (IR) injury is associated with poor outcome after liver transplantation and liver resections. Hexafluoroisopropanol (HFIP) is a tri‐fluorinated metabolites of volatile anesthetics and has modulatory effects on inflammation that have been observed mainly in cell culture experiments. In this survey, we investigated the effects of HFIP in a rat model of normothermic hepatic ischemia–reperfusion injury. Twenty‐four male Wistar rats were randomized into three groups: (1) control in which animals were submitted to 30 min of partial liver ischemia with resection of non‐ischemic liver lobes immediate after reperfusion, (2) pre‐ischemia (PI) group in which animals received intravenous HFIP (67 mg/kg) 5 min before liver ischemia, and (3) pre‐reperfusion (PR) group in which animals received intravenous HFIP (67 mg/kg) 5 min before reperfusion. Four hours after reperfusion, all animals were euthanized for sample collection. Aspartate and alanine transaminases, glucose, and high mobility group box‐1 (HMGB‐1) protein concentrations showed a significant decreased, and malondialdehyde was increased in the PR group compared with control and PI groups. Interleukin 6 (IL‐6) was increased in the PI group compared with control and PR groups. IL‐10 and ‐12 were increased in the PR and PI groups, respectively, when compared with the control group. Glucose decreased in the PR when compared with the control group. Post‐conditioning with HFIP led to a decrease in hepatocellular injury and was associated with a downregulation of HMGB‐1. The HFIP resulted in a better control of inflammatory response to ischemia–reperfusion even without causing a reduction in oxidative stress.https://doi.org/10.1002/prp2.1027hexafluoroisopropanolischemiaischemic postconditioningischemic preconditioningliverreperfusion injury |
| spellingShingle | Agustin Vintimilla Moscoso Estela Regina Ramos Figueira Joel Avancini Rocha‐Filho Martin Urner Cinthia Lanchotte Jose Jukemura Jorge Luiz Saraiva Ximenes Sergio Carlos Nahas Luiz Augusto Carneiro D'Albuquerque Flavio Henrique Ferreira Galvao Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein Pharmacology Research & Perspectives hexafluoroisopropanol ischemia ischemic postconditioning ischemic preconditioning liver reperfusion injury |
| title | Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein |
| title_full | Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein |
| title_fullStr | Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein |
| title_full_unstemmed | Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein |
| title_short | Hexafluoroisopropanol decreases liver ischemia–reperfusion injury by downregulation of high mobility group box‐1 protein |
| title_sort | hexafluoroisopropanol decreases liver ischemia reperfusion injury by downregulation of high mobility group box 1 protein |
| topic | hexafluoroisopropanol ischemia ischemic postconditioning ischemic preconditioning liver reperfusion injury |
| url | https://doi.org/10.1002/prp2.1027 |
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