Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study
Background: Microbiological studies have shown that Candida albicans is the most commonly found fungi, ranging from 7% to 18% of the root canal infections of tooth. Various intracanal medicaments were analyzed to know the best among them. Objectives: The study objective was to evaluate and compare t...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | SRM Journal of Research in Dental Sciences |
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| Online Access: | http://www.srmjrds.in/article.asp?issn=0976-433X;year=2020;volume=11;issue=2;spage=82;epage=86;aulast=Paul |
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| author | Prenu Paul T P Chandru Faizal C Peedikayil Gufran Ahmed Soni Kottayi Najma Azhikodan |
| author_facet | Prenu Paul T P Chandru Faizal C Peedikayil Gufran Ahmed Soni Kottayi Najma Azhikodan |
| author_sort | Prenu Paul |
| collection | DOAJ |
| description | Background: Microbiological studies have shown that Candida albicans is the most commonly found fungi, ranging from 7% to 18% of the root canal infections of tooth. Various intracanal medicaments were analyzed to know the best among them. Objectives: The study objective was to evaluate and compare the antifungal efficacy of various intracanal medicaments, i.e., calcium hydroxide, Odontopaste®, Ledermix® paste, Pulpdent® paste, tri-antibiotic paste, and Coconut® oil cream on C. albicans Materials and Methods: Seventy freshly extracted human permanent maxillary and mandibular anterior teeth extracted for various therapeutic reasons were selected for the study. A standard strain of C. albicans (10,231) was selected and suspended in 20.0 ml of Sabouraud Dextrose broth, and the suspension was adjusted to match the turbidity equivalent to 0.5 McFarland standards. The dentinal blocks were inoculated with C. albicans strains. The seventy dentinal blocks were then divided into seven groups according to the intracanal medicaments used in the study. Each group comprised of ten specimens. Each group was further divided into two subgroups according to the experimental periods, i.e., day 1 and day 7. The data were tabulated and statistically analyzed using nonparametric Wilcoxon signed-rank test and Kruskal–Wallis test to compare the mean and median reduction of C. albicans between intracanal medicaments. P < 0.05 was considered statistically significant.Results: Among the tested medicaments, Odontopaste® and Ledermix® paste showed the maximum effectiveness throughout the experimental period followed by Pulpdent® paste. Conclusion: The medicaments used in this study showed antimicrobial activity even though complete elimination was not obtained with any of the medicaments. The present study also showed that Odontopaste® and Ledermix® paste were the most effective antimicrobial medicaments against C. albicans. More researches are necessary for improving the antifungal efficacy of the medication used nowadays as intracanal dressing. |
| first_indexed | 2024-04-11T12:30:11Z |
| format | Article |
| id | doaj.art-765cc31607af404f90af0ac5829a7d32 |
| institution | Directory Open Access Journal |
| issn | 2772-5243 2772-5251 |
| language | English |
| last_indexed | 2024-04-11T12:30:11Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | SRM Journal of Research in Dental Sciences |
| spelling | doaj.art-765cc31607af404f90af0ac5829a7d322022-12-22T04:23:47ZengWolters Kluwer Medknow PublicationsSRM Journal of Research in Dental Sciences2772-52432772-52512020-01-01112828610.4103/srmjrds.srmjrds_20_20Effectiveness of various intracanal medicaments on Candida albicans: An in vitro studyPrenu PaulT P ChandruFaizal C PeedikayilGufran AhmedSoni KottayiNajma AzhikodanBackground: Microbiological studies have shown that Candida albicans is the most commonly found fungi, ranging from 7% to 18% of the root canal infections of tooth. Various intracanal medicaments were analyzed to know the best among them. Objectives: The study objective was to evaluate and compare the antifungal efficacy of various intracanal medicaments, i.e., calcium hydroxide, Odontopaste®, Ledermix® paste, Pulpdent® paste, tri-antibiotic paste, and Coconut® oil cream on C. albicans Materials and Methods: Seventy freshly extracted human permanent maxillary and mandibular anterior teeth extracted for various therapeutic reasons were selected for the study. A standard strain of C. albicans (10,231) was selected and suspended in 20.0 ml of Sabouraud Dextrose broth, and the suspension was adjusted to match the turbidity equivalent to 0.5 McFarland standards. The dentinal blocks were inoculated with C. albicans strains. The seventy dentinal blocks were then divided into seven groups according to the intracanal medicaments used in the study. Each group comprised of ten specimens. Each group was further divided into two subgroups according to the experimental periods, i.e., day 1 and day 7. The data were tabulated and statistically analyzed using nonparametric Wilcoxon signed-rank test and Kruskal–Wallis test to compare the mean and median reduction of C. albicans between intracanal medicaments. P < 0.05 was considered statistically significant.Results: Among the tested medicaments, Odontopaste® and Ledermix® paste showed the maximum effectiveness throughout the experimental period followed by Pulpdent® paste. Conclusion: The medicaments used in this study showed antimicrobial activity even though complete elimination was not obtained with any of the medicaments. The present study also showed that Odontopaste® and Ledermix® paste were the most effective antimicrobial medicaments against C. albicans. More researches are necessary for improving the antifungal efficacy of the medication used nowadays as intracanal dressing.http://www.srmjrds.in/article.asp?issn=0976-433X;year=2020;volume=11;issue=2;spage=82;epage=86;aulast=Paulcalcium hydroxidecandida albicanscoconut oilintracanal medicamentsledermix® pasteodontopaste®pulpdent® paste |
| spellingShingle | Prenu Paul T P Chandru Faizal C Peedikayil Gufran Ahmed Soni Kottayi Najma Azhikodan Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study SRM Journal of Research in Dental Sciences calcium hydroxide candida albicans coconut oil intracanal medicaments ledermix® paste odontopaste® pulpdent® paste |
| title | Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study |
| title_full | Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study |
| title_fullStr | Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study |
| title_full_unstemmed | Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study |
| title_short | Effectiveness of various intracanal medicaments on Candida albicans: An in vitro study |
| title_sort | effectiveness of various intracanal medicaments on candida albicans an in vitro study |
| topic | calcium hydroxide candida albicans coconut oil intracanal medicaments ledermix® paste odontopaste® pulpdent® paste |
| url | http://www.srmjrds.in/article.asp?issn=0976-433X;year=2020;volume=11;issue=2;spage=82;epage=86;aulast=Paul |
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