Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i>
Phytochemical investigation and chromatographic separation of extracts from one new actinobacteria strain <i>Amycolatopsis taiwanensis</i> that was isolated from soil of Yilan township, in the north of Taiwan, led to the isolation of nine new compounds, amycolataiwanensins A–I (<b>...
Main Authors: | , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2021-09-01
|
Series: | Molecules |
Subjects: | |
Online Access: | https://www.mdpi.com/1420-3049/26/19/5765 |
_version_ | 1797515994897317888 |
---|---|
author | Yung-Shun Su Ming-Der Wu Jih-Jung Chen Ming-Jen Cheng Yueh-Hsiung Kuo Chee-Yin Chai Aij-Lie Kwan |
author_facet | Yung-Shun Su Ming-Der Wu Jih-Jung Chen Ming-Jen Cheng Yueh-Hsiung Kuo Chee-Yin Chai Aij-Lie Kwan |
author_sort | Yung-Shun Su |
collection | DOAJ |
description | Phytochemical investigation and chromatographic separation of extracts from one new actinobacteria strain <i>Amycolatopsis taiwanensis</i> that was isolated from soil of Yilan township, in the north of Taiwan, led to the isolation of nine new compounds, amycolataiwanensins A–I (<b>1</b>–<b>9</b>, resp.), and one <i>new natural product</i>, namely amycolataiwanensin J (<b>10</b>). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic-data analysis (1D- and 2D-NMR, MS, and UV) and comparison with literature data. The effect of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages was evaluated. Of the isolates, <b>3</b>, <b>5</b>, <b>7</b> and <b>8</b> exhibited potent anti-NO production activity, with <i>IC</i><sub>50</sub> values of 17.52, 12.31, 17.81 and 13.32 μM, respectively, compared to that of quercetin, an iNOS inhibitor with an IC<sub>50</sub> value of 35.94 μM. This is the first report on indole metabolite from the genus <i>Amycolatopsis</i>. |
first_indexed | 2024-03-10T06:55:03Z |
format | Article |
id | doaj.art-765e5670f4874252bc930dbeadf9b7d2 |
institution | Directory Open Access Journal |
issn | 1420-3049 |
language | English |
last_indexed | 2024-03-10T06:55:03Z |
publishDate | 2021-09-01 |
publisher | MDPI AG |
record_format | Article |
series | Molecules |
spelling | doaj.art-765e5670f4874252bc930dbeadf9b7d22023-11-22T16:32:20ZengMDPI AGMolecules1420-30492021-09-012619576510.3390/molecules26195765Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i>Yung-Shun Su0Ming-Der Wu1Jih-Jung Chen2Ming-Jen Cheng3Yueh-Hsiung Kuo4Chee-Yin Chai5Aij-Lie Kwan6Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University (KMU), Kaohsiung 807, TaiwanBioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, TaiwanDepartment of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University (NYCU), Taipei 112, TaiwanBioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 300, TaiwanDepartment of Chemistry, National Taiwan University, Taipei 106, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University (KMU), Kaohsiung 807, TaiwanGraduate Institute of Medicine, College of Medicine, Kaohsiung Medical University (KMU), Kaohsiung 807, TaiwanPhytochemical investigation and chromatographic separation of extracts from one new actinobacteria strain <i>Amycolatopsis taiwanensis</i> that was isolated from soil of Yilan township, in the north of Taiwan, led to the isolation of nine new compounds, amycolataiwanensins A–I (<b>1</b>–<b>9</b>, resp.), and one <i>new natural product</i>, namely amycolataiwanensin J (<b>10</b>). The structures of the new compounds were unambiguously elucidated on the basis of extensive spectroscopic-data analysis (1D- and 2D-NMR, MS, and UV) and comparison with literature data. The effect of some isolates on the inhibition of NO production in lipopolysaccharide-activated RAW 264.7 murine macrophages was evaluated. Of the isolates, <b>3</b>, <b>5</b>, <b>7</b> and <b>8</b> exhibited potent anti-NO production activity, with <i>IC</i><sub>50</sub> values of 17.52, 12.31, 17.81 and 13.32 μM, respectively, compared to that of quercetin, an iNOS inhibitor with an IC<sub>50</sub> value of 35.94 μM. This is the first report on indole metabolite from the genus <i>Amycolatopsis</i>.https://www.mdpi.com/1420-3049/26/19/5765<i>Amycolatopsis taiwanensis</i>Pseudonocardiaceaeactinobacteriasecondary metabolitesNO inhibition |
spellingShingle | Yung-Shun Su Ming-Der Wu Jih-Jung Chen Ming-Jen Cheng Yueh-Hsiung Kuo Chee-Yin Chai Aij-Lie Kwan Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i> Molecules <i>Amycolatopsis taiwanensis</i> Pseudonocardiaceae actinobacteria secondary metabolites NO inhibition |
title | Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i> |
title_full | Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i> |
title_fullStr | Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i> |
title_full_unstemmed | Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i> |
title_short | Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria <i>Amycolatopsis taiwanensis</i> |
title_sort | secondary metabolites with anti inflammatory activities from one actinobacteria i amycolatopsis taiwanensis i |
topic | <i>Amycolatopsis taiwanensis</i> Pseudonocardiaceae actinobacteria secondary metabolites NO inhibition |
url | https://www.mdpi.com/1420-3049/26/19/5765 |
work_keys_str_mv | AT yungshunsu secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi AT mingderwu secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi AT jihjungchen secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi AT mingjencheng secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi AT yuehhsiungkuo secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi AT cheeyinchai secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi AT aijliekwan secondarymetaboliteswithantiinflammatoryactivitiesfromoneactinobacteriaiamycolatopsistaiwanensisi |