Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone
An innovative approach to treating bone defects is using synthetic bone substitutes made of biomaterials. The proposed method to obtain polylactide scaffolds using the phase inversion technique with a freeze extraction variant enables the production of substitutes with morphology similar to cancello...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/8/410 |
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| author | Anna Kołakowska Dorota Kołbuk Andrzej Chwojnowski Andrzej Rafalski Agnieszka Gadomska-Gajadhur |
| author_facet | Anna Kołakowska Dorota Kołbuk Andrzej Chwojnowski Andrzej Rafalski Agnieszka Gadomska-Gajadhur |
| author_sort | Anna Kołakowska |
| collection | DOAJ |
| description | An innovative approach to treating bone defects is using synthetic bone substitutes made of biomaterials. The proposed method to obtain polylactide scaffolds using the phase inversion technique with a freeze extraction variant enables the production of substitutes with morphology similar to cancellous bone (pore size 100–400 µm, open porosity 94%). The high absorbability of the implants will enable their use as platelet-rich plasma (PRP) carriers in future medical devices. Surface modification by dipping enabled the deposition of the hydrophilic chitosan (CS) layer, maintaining good bone tissue properties and high absorbability (850% dry weight). Introducing CS increases surface roughness and causes local changes in surface free energy, promoting bone cell adhesion. Through this research, we have developed a new and original method of low-temperature modification of PLA substitutes with chitosan. This method uses non-toxic reagents that do not cause changes in the structure of the PLA matrix. The obtained bone substitutes are characterised by exceptionally high hydrophilicity and morphology similar to spongy bone. In vitro studies were performed to analyse the effect of morphology and chitosan on cellular viability. Substitutes with properties similar to those of cancellous bone and which promote bone cell growth were obtained. |
| first_indexed | 2024-03-10T23:50:32Z |
| format | Article |
| id | doaj.art-76608721c48c45af888da7c529ca0a65 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T23:50:32Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-76608721c48c45af888da7c529ca0a652023-11-19T01:43:06ZengMDPI AGJournal of Functional Biomaterials2079-49832023-08-0114841010.3390/jfb14080410Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous BoneAnna Kołakowska0Dorota Kołbuk1Andrzej Chwojnowski2Andrzej Rafalski3Agnieszka Gadomska-Gajadhur4Faculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664 Warsaw, PolandInstitute of Fundamental Technological Research Polish Academy of Sciences, Pawińskiego St. 5B, 02-106 Warsaw, PolandNalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Ks. Trojdena St. 4, 02-109 Warsaw, PolandRadiosterilisation Facility for Medical Supplies and Transplants, Institute Nuclear Chemistry and Technology, Dorodna St. 16, 03-195 Warsaw, PolandFaculty of Chemistry, Warsaw University of Technology, Noakowskiego St. 3, 00-664 Warsaw, PolandAn innovative approach to treating bone defects is using synthetic bone substitutes made of biomaterials. The proposed method to obtain polylactide scaffolds using the phase inversion technique with a freeze extraction variant enables the production of substitutes with morphology similar to cancellous bone (pore size 100–400 µm, open porosity 94%). The high absorbability of the implants will enable their use as platelet-rich plasma (PRP) carriers in future medical devices. Surface modification by dipping enabled the deposition of the hydrophilic chitosan (CS) layer, maintaining good bone tissue properties and high absorbability (850% dry weight). Introducing CS increases surface roughness and causes local changes in surface free energy, promoting bone cell adhesion. Through this research, we have developed a new and original method of low-temperature modification of PLA substitutes with chitosan. This method uses non-toxic reagents that do not cause changes in the structure of the PLA matrix. The obtained bone substitutes are characterised by exceptionally high hydrophilicity and morphology similar to spongy bone. In vitro studies were performed to analyse the effect of morphology and chitosan on cellular viability. Substitutes with properties similar to those of cancellous bone and which promote bone cell growth were obtained.https://www.mdpi.com/2079-4983/14/8/410chitosanpolylactidebone substitutetissue regeneration |
| spellingShingle | Anna Kołakowska Dorota Kołbuk Andrzej Chwojnowski Andrzej Rafalski Agnieszka Gadomska-Gajadhur Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone Journal of Functional Biomaterials chitosan polylactide bone substitute tissue regeneration |
| title | Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone |
| title_full | Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone |
| title_fullStr | Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone |
| title_full_unstemmed | Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone |
| title_short | Chitosan-Based High-Intensity Modification of the Biodegradable Substitutes for Cancellous Bone |
| title_sort | chitosan based high intensity modification of the biodegradable substitutes for cancellous bone |
| topic | chitosan polylactide bone substitute tissue regeneration |
| url | https://www.mdpi.com/2079-4983/14/8/410 |
| work_keys_str_mv | AT annakołakowska chitosanbasedhighintensitymodificationofthebiodegradablesubstitutesforcancellousbone AT dorotakołbuk chitosanbasedhighintensitymodificationofthebiodegradablesubstitutesforcancellousbone AT andrzejchwojnowski chitosanbasedhighintensitymodificationofthebiodegradablesubstitutesforcancellousbone AT andrzejrafalski chitosanbasedhighintensitymodificationofthebiodegradablesubstitutesforcancellousbone AT agnieszkagadomskagajadhur chitosanbasedhighintensitymodificationofthebiodegradablesubstitutesforcancellousbone |