Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing
Despite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/11/6/977 |
| _version_ | 1797532263706001408 |
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| author | Jaehyung Jeon Chanyong Park Dinesh Veeran Ponnuvelu Sungsu Park |
| author_facet | Jaehyung Jeon Chanyong Park Dinesh Veeran Ponnuvelu Sungsu Park |
| author_sort | Jaehyung Jeon |
| collection | DOAJ |
| description | Despite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed channels using vat photopolymerization to avoid fluid evaporation. In detail, height of the enclosed channels was adjusted from 0.3 to 0.17 mm by varying the UV exposure time from 1 to 4 s for the top barrier, whereas the exposure time for the bottom and side barriers was fixed. As a result, sample flow in the enclosed channels of 3D-μPADs showed lesser wicking speed with very scant evaporation compared to that in the hemi channels in the 3D-μPADs. The stoppage of evaporation in the enclosed channels significantly improved the gray intensity and uniformity in the detection zone of the 3D-μPADs, resulting in as low as 0.3 mM glucose detection. Thus 3D-μPADs with enclosed channels showed enhanced sensitivity compared to the 3D-μPADs with hemi channels when dealing with a small volume sample. Our work provides a new insight into 3D-μPAD design with enclosed channels, which redefines the methodology in 3D printing. |
| first_indexed | 2024-03-10T10:56:38Z |
| format | Article |
| id | doaj.art-76625650e3414baa8f0e9edb25476b0c |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T10:56:38Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-76625650e3414baa8f0e9edb25476b0c2023-11-21T21:50:22ZengMDPI AGDiagnostics2075-44182021-05-0111697710.3390/diagnostics11060977Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care TestingJaehyung Jeon0Chanyong Park1Dinesh Veeran Ponnuvelu2Sungsu Park3School of Mechanical Engineering, Sungkyunkwan University, Seoburo 2066, Jangan-gu, Suwon 16419, KoreaSchool of Mechanical Engineering, Sungkyunkwan University, Seoburo 2066, Jangan-gu, Suwon 16419, KoreaSchool of Mechanical Engineering, Sungkyunkwan University, Seoburo 2066, Jangan-gu, Suwon 16419, KoreaSchool of Mechanical Engineering, Sungkyunkwan University, Seoburo 2066, Jangan-gu, Suwon 16419, KoreaDespite the potential in fabrication of microfluidic paper-based analytical devices (μPADs) for point-of-care testing (POCT) kits, the development of simple, accurate, and rapid devices with higher sensitivity remains challenging. Here, we report a novel method for 3D-μPAD fabrication with enclosed channels using vat photopolymerization to avoid fluid evaporation. In detail, height of the enclosed channels was adjusted from 0.3 to 0.17 mm by varying the UV exposure time from 1 to 4 s for the top barrier, whereas the exposure time for the bottom and side barriers was fixed. As a result, sample flow in the enclosed channels of 3D-μPADs showed lesser wicking speed with very scant evaporation compared to that in the hemi channels in the 3D-μPADs. The stoppage of evaporation in the enclosed channels significantly improved the gray intensity and uniformity in the detection zone of the 3D-μPADs, resulting in as low as 0.3 mM glucose detection. Thus 3D-μPADs with enclosed channels showed enhanced sensitivity compared to the 3D-μPADs with hemi channels when dealing with a small volume sample. Our work provides a new insight into 3D-μPAD design with enclosed channels, which redefines the methodology in 3D printing.https://www.mdpi.com/2075-4418/11/6/977paper-based analytical devices3D printingenclosed channelsmall-volume sampleevaporationpoint-of-care testing |
| spellingShingle | Jaehyung Jeon Chanyong Park Dinesh Veeran Ponnuvelu Sungsu Park Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing Diagnostics paper-based analytical devices 3D printing enclosed channel small-volume sample evaporation point-of-care testing |
| title | Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing |
| title_full | Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing |
| title_fullStr | Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing |
| title_full_unstemmed | Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing |
| title_short | Enhanced Sensing Behavior of Three-Dimensional Microfluidic Paper-Based Analytical Devices (3D-μPADs) with Evaporation-Free Enclosed Channels for Point-of-Care Testing |
| title_sort | enhanced sensing behavior of three dimensional microfluidic paper based analytical devices 3d μpads with evaporation free enclosed channels for point of care testing |
| topic | paper-based analytical devices 3D printing enclosed channel small-volume sample evaporation point-of-care testing |
| url | https://www.mdpi.com/2075-4418/11/6/977 |
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