Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway
Background/Aims: Periapical periodontitis is caused by bacterial infection and results in both one destruction and tooth loss. Osteopontin (OPN) is a secreted phosphorylated glycoprotein that participates in bone metabolism. Methods: Thirty-three patients with chronic periapical periodontitis and 10...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2018-09-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/493219 |
| _version_ | 1818275637581840384 |
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| author | Ming Dong Xinxin Yu Wanfang Chen Zhenzhen Guo Linlin Sui Yuefei Xu Yuhong Shang Weidong Niu Ying Kong |
| author_facet | Ming Dong Xinxin Yu Wanfang Chen Zhenzhen Guo Linlin Sui Yuefei Xu Yuhong Shang Weidong Niu Ying Kong |
| author_sort | Ming Dong |
| collection | DOAJ |
| description | Background/Aims: Periapical periodontitis is caused by bacterial infection and results in both one destruction and tooth loss. Osteopontin (OPN) is a secreted phosphorylated glycoprotein that participates in bone metabolism. Methods: Thirty-three patients with chronic periapical periodontitis and 10 patients who had undergone the orthodontic removal of healthy tooth tissue (control) at the periodontal ligament were investigated, and an animal model of mouse periapical periodontitis was established for an in vivo analysis. The relationship between OPN and bone destruction during periapical periodontitis was analyzed. Osteoblasts and osteoclasts were cultured in vitro and treated with lipopolysaccharide. An inhibitor of NF-κB was used to pretreat the transfected cells. Results: OPN increased osteoclast proliferation and differentiation, but reduced osteoblasts proliferation and differentiation. OPN activated the NF-κB pathway during periapical periodontitis and accelerated the transfer and phosphorylation of P65 from the cytoplasm to the nucleus. Conclusion: This study demonstrated that OPN played important roles in the progression of periapical periodontitis, and a dual role in bone metabolism during periapical periodontitis, linking osteoclasts and osteoblasts. The underlying mechanism may be related to the NF-κB pathway. |
| first_indexed | 2024-12-12T22:32:55Z |
| format | Article |
| id | doaj.art-76668945b8f044ee97dfc4a159b8cbe6 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-12T22:32:55Z |
| publishDate | 2018-09-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-76668945b8f044ee97dfc4a159b8cbe62022-12-22T00:09:33ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-09-0149388489810.1159/000493219493219Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB PathwayMing DongXinxin YuWanfang ChenZhenzhen GuoLinlin SuiYuefei XuYuhong ShangWeidong NiuYing KongBackground/Aims: Periapical periodontitis is caused by bacterial infection and results in both one destruction and tooth loss. Osteopontin (OPN) is a secreted phosphorylated glycoprotein that participates in bone metabolism. Methods: Thirty-three patients with chronic periapical periodontitis and 10 patients who had undergone the orthodontic removal of healthy tooth tissue (control) at the periodontal ligament were investigated, and an animal model of mouse periapical periodontitis was established for an in vivo analysis. The relationship between OPN and bone destruction during periapical periodontitis was analyzed. Osteoblasts and osteoclasts were cultured in vitro and treated with lipopolysaccharide. An inhibitor of NF-κB was used to pretreat the transfected cells. Results: OPN increased osteoclast proliferation and differentiation, but reduced osteoblasts proliferation and differentiation. OPN activated the NF-κB pathway during periapical periodontitis and accelerated the transfer and phosphorylation of P65 from the cytoplasm to the nucleus. Conclusion: This study demonstrated that OPN played important roles in the progression of periapical periodontitis, and a dual role in bone metabolism during periapical periodontitis, linking osteoclasts and osteoblasts. The underlying mechanism may be related to the NF-κB pathway.https://www.karger.com/Article/FullText/493219OsteopontinBone absorptionPeriapical periodontitisNF-κB |
| spellingShingle | Ming Dong Xinxin Yu Wanfang Chen Zhenzhen Guo Linlin Sui Yuefei Xu Yuhong Shang Weidong Niu Ying Kong Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway Cellular Physiology and Biochemistry Osteopontin Bone absorption Periapical periodontitis NF-κB |
| title | Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway |
| title_full | Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway |
| title_fullStr | Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway |
| title_full_unstemmed | Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway |
| title_short | Osteopontin Promotes Bone Destruction in Periapical Periodontitis by Activating the NF-κB Pathway |
| title_sort | osteopontin promotes bone destruction in periapical periodontitis by activating the nf κb pathway |
| topic | Osteopontin Bone absorption Periapical periodontitis NF-κB |
| url | https://www.karger.com/Article/FullText/493219 |
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