Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement
The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2010-05-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020 |
| _version_ | 1797757191481982976 |
|---|---|
| author | Juliana Magalhães Vital-Brazil Ilana Teruszkin Balassiano Fabiano Sutter de Oliveira Alberto Dias de Souza Costa Leandro Hillen Martha Maria Pereira |
| author_facet | Juliana Magalhães Vital-Brazil Ilana Teruszkin Balassiano Fabiano Sutter de Oliveira Alberto Dias de Souza Costa Leandro Hillen Martha Maria Pereira |
| author_sort | Juliana Magalhães Vital-Brazil |
| collection | DOAJ |
| description | The aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies. |
| first_indexed | 2024-03-12T18:11:25Z |
| format | Article |
| id | doaj.art-76670b6bec4f4b9389350a3b38f370a6 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:11:25Z |
| publishDate | 2010-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-76670b6bec4f4b9389350a3b38f370a62023-08-02T09:15:23ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-05-01105335335510.1590/S0074-02762010000300020Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlementJuliana Magalhães Vital-BrazilIlana Teruszkin BalassianoFabiano Sutter de OliveiraAlberto Dias de Souza CostaLeandro HillenMartha Maria PereiraThe aim of this study was to apply a molecular protocol to detect leptospiral DNA in environmental water samples. The study was carried out in a peri-urban settlement in Petrópolis, state of Rio de Janeiro. A multiplex PCR method employing the primers LipL32 and 16SrRNA was used. Three out of 100 analysed samples were positive in the multiplex PCR, two were considered to have saprophytic leptospires and one had pathogenic leptospires. The results obtained supported the idea that multiplex PCR can be used to detect Leptospira spp in water samples. This method was also able to differentiate between saprophytic and pathogenic leptospires and was able to do so much more easily than conventional methodologies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020Leptospira sppmultiplex PCRwater sample |
| spellingShingle | Juliana Magalhães Vital-Brazil Ilana Teruszkin Balassiano Fabiano Sutter de Oliveira Alberto Dias de Souza Costa Leandro Hillen Martha Maria Pereira Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement Memorias do Instituto Oswaldo Cruz Leptospira spp multiplex PCR water sample |
| title | Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement |
| title_full | Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement |
| title_fullStr | Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement |
| title_full_unstemmed | Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement |
| title_short | Multiplex PCR-based detection of Leptospira in environmental water samples obtained from a slum settlement |
| title_sort | multiplex pcr based detection of leptospira in environmental water samples obtained from a slum settlement |
| topic | Leptospira spp multiplex PCR water sample |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300020 |
| work_keys_str_mv | AT julianamagalhaesvitalbrazil multiplexpcrbaseddetectionofleptospirainenvironmentalwatersamplesobtainedfromaslumsettlement AT ilanateruszkinbalassiano multiplexpcrbaseddetectionofleptospirainenvironmentalwatersamplesobtainedfromaslumsettlement AT fabianosutterdeoliveira multiplexpcrbaseddetectionofleptospirainenvironmentalwatersamplesobtainedfromaslumsettlement AT albertodiasdesouzacosta multiplexpcrbaseddetectionofleptospirainenvironmentalwatersamplesobtainedfromaslumsettlement AT leandrohillen multiplexpcrbaseddetectionofleptospirainenvironmentalwatersamplesobtainedfromaslumsettlement AT marthamariapereira multiplexpcrbaseddetectionofleptospirainenvironmentalwatersamplesobtainedfromaslumsettlement |