High-fat-diet-induced gut microbiome changes in mice
Obesity is one of the most severe public health problems and may result in many other related diseases, such as heart disease, diabetes, and stroke. Living habits, particularly excessive caloric intake, are a vital contributor to increasing obesity incidence worldwide. Recent studies have revealed a...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-06-01
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| Series: | Stress and Brain |
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| Online Access: | https://www.sciopen.com/article/10.26599/SAB.2022.9060012 |
| _version_ | 1811175556773838848 |
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| author | Wenqi Tang Libiao Pan Jingjing Cheng Xi Wang Lu Zheng Siyu Wang Yudong Zhou Hao Wang |
| author_facet | Wenqi Tang Libiao Pan Jingjing Cheng Xi Wang Lu Zheng Siyu Wang Yudong Zhou Hao Wang |
| author_sort | Wenqi Tang |
| collection | DOAJ |
| description | Obesity is one of the most severe public health problems and may result in many other related diseases, such as heart disease, diabetes, and stroke. Living habits, particularly excessive caloric intake, are a vital contributor to increasing obesity incidence worldwide. Recent studies have revealed an intimate cross-talk between living habits and the gut microbiome. The gut microbiome has also been identified as a critical player in the cause of obesity. Here we used 16S rDNA amplicon sequencing to investigate changes in the gut microbiome composition of mice fed with a high-fat diet (HFD). The total number of OTUs, Chao index, and Shannon index of the gut microbiome showed an increase in the abundance of specific gut microbiome species. Alternatively, Simpson index indicated a decrease in gut microbiome diversity after HFD feeding. We also found that HFD leads to augments in Firmicutes:Bacteroidetes ratio mainly caused by increased Firmicutes. The total abundance of Bacteroidetes was not changed at the phylum level, while at the family level, both Rikenellaceae and Bacteroidaceae showed a significant increase after the HFD. Additionally, after HFD, we found an increase in the abundance of Proteobacteria related to inflammation and a significant decrease in the proportion of Verrucomicrobia. Our results show that HFD induces a broad gut microbiome change in mice. |
| first_indexed | 2024-04-10T19:37:43Z |
| format | Article |
| id | doaj.art-76672739dd49468ab88e3edc01b3a1a1 |
| institution | Directory Open Access Journal |
| issn | 2709-1325 |
| language | English |
| last_indexed | 2024-04-10T19:37:43Z |
| publishDate | 2022-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Stress and Brain |
| spelling | doaj.art-76672739dd49468ab88e3edc01b3a1a12023-01-30T04:00:57ZengTsinghua University PressStress and Brain2709-13252022-06-0121-2173010.26599/SAB.2022.9060012High-fat-diet-induced gut microbiome changes in miceWenqi Tang0Libiao Pan1Jingjing Cheng2Xi Wang3Lu Zheng4Siyu Wang5Yudong Zhou6Hao Wang7Department of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaDepartment of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Key Laboratory for Biomedical Engineering of Education Ministry, Zhejiang University School of Medicine, Hangzhou 310058, ChinaObesity is one of the most severe public health problems and may result in many other related diseases, such as heart disease, diabetes, and stroke. Living habits, particularly excessive caloric intake, are a vital contributor to increasing obesity incidence worldwide. Recent studies have revealed an intimate cross-talk between living habits and the gut microbiome. The gut microbiome has also been identified as a critical player in the cause of obesity. Here we used 16S rDNA amplicon sequencing to investigate changes in the gut microbiome composition of mice fed with a high-fat diet (HFD). The total number of OTUs, Chao index, and Shannon index of the gut microbiome showed an increase in the abundance of specific gut microbiome species. Alternatively, Simpson index indicated a decrease in gut microbiome diversity after HFD feeding. We also found that HFD leads to augments in Firmicutes:Bacteroidetes ratio mainly caused by increased Firmicutes. The total abundance of Bacteroidetes was not changed at the phylum level, while at the family level, both Rikenellaceae and Bacteroidaceae showed a significant increase after the HFD. Additionally, after HFD, we found an increase in the abundance of Proteobacteria related to inflammation and a significant decrease in the proportion of Verrucomicrobia. Our results show that HFD induces a broad gut microbiome change in mice.https://www.sciopen.com/article/10.26599/SAB.2022.9060012gut microbiome16s rdnahigh-fat dietfirmicutesbacteroidaceae |
| spellingShingle | Wenqi Tang Libiao Pan Jingjing Cheng Xi Wang Lu Zheng Siyu Wang Yudong Zhou Hao Wang High-fat-diet-induced gut microbiome changes in mice Stress and Brain gut microbiome 16s rdna high-fat diet firmicutes bacteroidaceae |
| title | High-fat-diet-induced gut microbiome changes in mice |
| title_full | High-fat-diet-induced gut microbiome changes in mice |
| title_fullStr | High-fat-diet-induced gut microbiome changes in mice |
| title_full_unstemmed | High-fat-diet-induced gut microbiome changes in mice |
| title_short | High-fat-diet-induced gut microbiome changes in mice |
| title_sort | high fat diet induced gut microbiome changes in mice |
| topic | gut microbiome 16s rdna high-fat diet firmicutes bacteroidaceae |
| url | https://www.sciopen.com/article/10.26599/SAB.2022.9060012 |
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