Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States
Recent advances in molecular sequencing technology and the increased availability of fieldable laboratory equipment have provided researchers with the opportunity to conduct real-time or near real-time gene-based biodiversity assessments of aquatic ecosystems. In this study, we developed a workflow...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.640527/full |
| _version_ | 1818412365391069184 |
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| author | Cheryl Lewis Ames Cheryl Lewis Ames Cheryl Lewis Ames Aki H. Ohdera Aki H. Ohdera Sophie M. Colston Allen G. Collins Allen G. Collins William K. Fitt André C. Morandini André C. Morandini Jeffrey S. Erickson Gary J. Vora |
| author_facet | Cheryl Lewis Ames Cheryl Lewis Ames Cheryl Lewis Ames Aki H. Ohdera Aki H. Ohdera Sophie M. Colston Allen G. Collins Allen G. Collins William K. Fitt André C. Morandini André C. Morandini Jeffrey S. Erickson Gary J. Vora |
| author_sort | Cheryl Lewis Ames |
| collection | DOAJ |
| description | Recent advances in molecular sequencing technology and the increased availability of fieldable laboratory equipment have provided researchers with the opportunity to conduct real-time or near real-time gene-based biodiversity assessments of aquatic ecosystems. In this study, we developed a workflow and portable kit for fieldable environmental DNA sequencing (FeDS) and tested its efficacy by characterizing the breadth of jellyfish (Medusozoa) taxa in the coastal waters of the Upper and Lower Florida Keys. Environmental DNA was isolated from seawater collection events at eight sites and samples were subjected to medusozoan 16S rRNA gene and metazoan mitochondrial cytochrome oxidase 1 gene profiling via metabarcoding onsite. In total, FeDS yielded 175,326 processed sequence reads providing evidence for 53 medusozoan taxa. Our most salient findings revealed eDNA from: (1) two venomous box jellyfish (Cubozoa) species, including taxa whose stings cause the notorious Irukandji envenomation syndrome; (2) two species of potentially introduced stalked jellyfish (Staurozoa); and (3) a likely cryptic species of upside-down jellyfish (Scyphozoa). Taken together, the results of this study highlight the merits of FeDS in conducting biodiversity surveys of endemic and introduced species, and as a potential tool for assessing envenomation and/or conservation-related threats. |
| first_indexed | 2024-12-14T10:46:09Z |
| format | Article |
| id | doaj.art-766ac0066cfc4e22b5cb9c49e179d2df |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-14T10:46:09Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-766ac0066cfc4e22b5cb9c49e179d2df2022-12-21T23:05:26ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-04-01810.3389/fmars.2021.640527640527Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United StatesCheryl Lewis Ames0Cheryl Lewis Ames1Cheryl Lewis Ames2Aki H. Ohdera3Aki H. Ohdera4Sophie M. Colston5Allen G. Collins6Allen G. Collins7William K. Fitt8André C. Morandini9André C. Morandini10Jeffrey S. Erickson11Gary J. Vora12National Research Council, National Academy of Sciences, U.S. Naval Research Laboratory, Washington, DC, United StatesGraduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Sendai, JapanDepartment of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United StatesDepartment of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United StatesDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, United StatesNational Research Council, National Academy of Sciences, U.S. Naval Research Laboratory, Washington, DC, United StatesDepartment of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United StatesNational Systematics Laboratory of the National Oceanic Atmospheric Administration Fisheries Service, National Museum of Natural History, Smithsonian Institution, Washington, DC, United StatesOdum School of Ecology, University of Georgia, Athens, GA, United StatesDepartamento de Zoologia, Instituto de Biociências, University of São Paulo, São Paulo, BrazilCentro de Biologia Marinha, University of São Paulo, São Sebastião, BrazilCenter for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC, United StatesCenter for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington, DC, United StatesRecent advances in molecular sequencing technology and the increased availability of fieldable laboratory equipment have provided researchers with the opportunity to conduct real-time or near real-time gene-based biodiversity assessments of aquatic ecosystems. In this study, we developed a workflow and portable kit for fieldable environmental DNA sequencing (FeDS) and tested its efficacy by characterizing the breadth of jellyfish (Medusozoa) taxa in the coastal waters of the Upper and Lower Florida Keys. Environmental DNA was isolated from seawater collection events at eight sites and samples were subjected to medusozoan 16S rRNA gene and metazoan mitochondrial cytochrome oxidase 1 gene profiling via metabarcoding onsite. In total, FeDS yielded 175,326 processed sequence reads providing evidence for 53 medusozoan taxa. Our most salient findings revealed eDNA from: (1) two venomous box jellyfish (Cubozoa) species, including taxa whose stings cause the notorious Irukandji envenomation syndrome; (2) two species of potentially introduced stalked jellyfish (Staurozoa); and (3) a likely cryptic species of upside-down jellyfish (Scyphozoa). Taken together, the results of this study highlight the merits of FeDS in conducting biodiversity surveys of endemic and introduced species, and as a potential tool for assessing envenomation and/or conservation-related threats.https://www.frontiersin.org/articles/10.3389/fmars.2021.640527/fulleDNAenvenomationupside-down jellyfishconservationbiodiversityportable lab kit |
| spellingShingle | Cheryl Lewis Ames Cheryl Lewis Ames Cheryl Lewis Ames Aki H. Ohdera Aki H. Ohdera Sophie M. Colston Allen G. Collins Allen G. Collins William K. Fitt André C. Morandini André C. Morandini Jeffrey S. Erickson Gary J. Vora Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States Frontiers in Marine Science eDNA envenomation upside-down jellyfish conservation biodiversity portable lab kit |
| title | Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States |
| title_full | Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States |
| title_fullStr | Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States |
| title_full_unstemmed | Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States |
| title_short | Fieldable Environmental DNA Sequencing to Assess Jellyfish Biodiversity in Nearshore Waters of the Florida Keys, United States |
| title_sort | fieldable environmental dna sequencing to assess jellyfish biodiversity in nearshore waters of the florida keys united states |
| topic | eDNA envenomation upside-down jellyfish conservation biodiversity portable lab kit |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.640527/full |
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