Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity
Abstract Background MicroRNAs (miRNAs) play an important regulatory role in mammalian reproduction. Currently, most studies are primarily concentrated on ovarian miRNAs, ignoring the influence of endometrial miRNAs on the fecundity of female sheep. To uncover potential regulators of sheep fecundity,...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09681-y |
| _version_ | 1797453815170990080 |
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| author | Jihong Dong Xuecheng Jiang Nan Liu Hegang Li Jinshan Zhao Jianning He Xiaoxiao Gao |
| author_facet | Jihong Dong Xuecheng Jiang Nan Liu Hegang Li Jinshan Zhao Jianning He Xiaoxiao Gao |
| author_sort | Jihong Dong |
| collection | DOAJ |
| description | Abstract Background MicroRNAs (miRNAs) play an important regulatory role in mammalian reproduction. Currently, most studies are primarily concentrated on ovarian miRNAs, ignoring the influence of endometrial miRNAs on the fecundity of female sheep. To uncover potential regulators of sheep fecundity, RNA-seq was used to comparatively analyze miRNA expression profiles of endometrium between high prolificacy sheep (HP, litter size = 3) and low prolificacy sheep (LP, litter size = 1) with FecB genotype. Results Firstly, genomic features of miRNAs from endometrium were analyzed. Furthermore, 58 differentially expressed (DE) miRNAs were found in the endometrium of Hu sheep with different litter size. A co-expression network of DE miRNAs and target genes has been constructed, and hub genes related litter size are included, such as DE miRNA unconservative_NC_019472.2_1229533 and unconservative_NC_019481.2_1637827 target to estrogen receptor α (ESR1) and unconservative_NC_019481.2_1637827 targets to transcription factor 7 (TCF7). Moreover, functional annotation analysis showed that the target genes (NRCAM and NEGR1) of the DE miRNAs were significantly enriched in cell adhesion molecules (CAMs) signaling pathway, which was related to uterine receptivity. Conclusion Taken together, this study provides a new valuable resource for understanding the molecular mechanisms underlying Hu sheep prolificacy. |
| first_indexed | 2024-03-09T15:27:16Z |
| format | Article |
| id | doaj.art-766b191ce11f462184ed1e0ae15b275c |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-09T15:27:16Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-766b191ce11f462184ed1e0ae15b275c2023-11-26T12:26:01ZengBMCBMC Genomics1471-21642023-10-0124111010.1186/s12864-023-09681-yIdentification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundityJihong Dong0Xuecheng Jiang1Nan Liu2Hegang Li3Jinshan Zhao4Jianning He5Xiaoxiao Gao6College of Animal Science and Technology, Qingdao Agricultural UniversityCollege of Animal Science and Technology, Qingdao Agricultural UniversityCollege of Animal Science and Technology, Qingdao Agricultural UniversityCollege of Animal Science and Technology, Qingdao Agricultural UniversityCollege of Animal Science and Technology, Qingdao Agricultural UniversityCollege of Animal Science and Technology, Qingdao Agricultural UniversityCollege of Animal Science and Technology, Qingdao Agricultural UniversityAbstract Background MicroRNAs (miRNAs) play an important regulatory role in mammalian reproduction. Currently, most studies are primarily concentrated on ovarian miRNAs, ignoring the influence of endometrial miRNAs on the fecundity of female sheep. To uncover potential regulators of sheep fecundity, RNA-seq was used to comparatively analyze miRNA expression profiles of endometrium between high prolificacy sheep (HP, litter size = 3) and low prolificacy sheep (LP, litter size = 1) with FecB genotype. Results Firstly, genomic features of miRNAs from endometrium were analyzed. Furthermore, 58 differentially expressed (DE) miRNAs were found in the endometrium of Hu sheep with different litter size. A co-expression network of DE miRNAs and target genes has been constructed, and hub genes related litter size are included, such as DE miRNA unconservative_NC_019472.2_1229533 and unconservative_NC_019481.2_1637827 target to estrogen receptor α (ESR1) and unconservative_NC_019481.2_1637827 targets to transcription factor 7 (TCF7). Moreover, functional annotation analysis showed that the target genes (NRCAM and NEGR1) of the DE miRNAs were significantly enriched in cell adhesion molecules (CAMs) signaling pathway, which was related to uterine receptivity. Conclusion Taken together, this study provides a new valuable resource for understanding the molecular mechanisms underlying Hu sheep prolificacy.https://doi.org/10.1186/s12864-023-09681-ymicroRNAsEndometriumFecundityHu sheep |
| spellingShingle | Jihong Dong Xuecheng Jiang Nan Liu Hegang Li Jinshan Zhao Jianning He Xiaoxiao Gao Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity BMC Genomics microRNAs Endometrium Fecundity Hu sheep |
| title | Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity |
| title_full | Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity |
| title_fullStr | Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity |
| title_full_unstemmed | Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity |
| title_short | Identification and analysis of differentially expressed microRNAs in endometrium to explore the regulation of sheep fecundity |
| title_sort | identification and analysis of differentially expressed micrornas in endometrium to explore the regulation of sheep fecundity |
| topic | microRNAs Endometrium Fecundity Hu sheep |
| url | https://doi.org/10.1186/s12864-023-09681-y |
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