Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury
Abstract Introduction The extracellular matrix (ECM) provides structural support for neuronal, glial, and vascular components of the brain, and regulates intercellular signaling required for cellular morphogenesis, differentiation and homeostasis. We hypothesize that the pathophysiology of diffuse b...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-09-01
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| Series: | Brain and Behavior |
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| Online Access: | https://doi.org/10.1002/brb3.1767 |
| _version_ | 1818448861458333696 |
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| author | Daniel R. Griffiths Taylor M. Jenkins Caroline P. Addington Sarah E. Stabenfeldt Jonathan Lifshitz |
| author_facet | Daniel R. Griffiths Taylor M. Jenkins Caroline P. Addington Sarah E. Stabenfeldt Jonathan Lifshitz |
| author_sort | Daniel R. Griffiths |
| collection | DOAJ |
| description | Abstract Introduction The extracellular matrix (ECM) provides structural support for neuronal, glial, and vascular components of the brain, and regulates intercellular signaling required for cellular morphogenesis, differentiation and homeostasis. We hypothesize that the pathophysiology of diffuse brain injury impacts the ECM in a multi‐dimensional way across brain regions and over time, which could facilitate damage and repair processes. Methods Experimental diffuse TBI was induced in male Sprague‐Dawley rats (325–375 g) by midline fluid percussion injury (FPI); uninjured sham rats serve as controls. Tissue from the cortex, thalamus, and hippocampus was collected at 15 min, 1, 2, 6, and 18 hr postinjury as well as 1, 3, 7, and 14 days postinjury. All samples were quantified by Western blot for glycoproteins: fibronectin, laminin, reelin, and tenascin‐C. Band intensities were normalized to sham and relative to β‐actin. Results In the cortex, fibronectin decreased significantly at 15 min, 1 hr, and 2 hr postinjury, while tenascin‐C decreased significantly at 7 and 14 days postinjury. In the thalamus, reelin decreased significantly at 2 hr, 3 and 14 days postinjury. In the hippocampus, tenascin‐C increased significantly at 15 min and 7 days postinjury. Conclusion Acute changes in the levels of these glycoproteins suggest involvement in circuit dismantling, whereas postacute levels may indicate a restorative or regenerative response associated with recovery from TBI. |
| first_indexed | 2024-12-14T20:26:14Z |
| format | Article |
| id | doaj.art-766b52c466bf42699c082d7b7852cc3a |
| institution | Directory Open Access Journal |
| issn | 2162-3279 |
| language | English |
| last_indexed | 2024-12-14T20:26:14Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Brain and Behavior |
| spelling | doaj.art-766b52c466bf42699c082d7b7852cc3a2022-12-21T22:48:37ZengWileyBrain and Behavior2162-32792020-09-01109n/an/a10.1002/brb3.1767Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injuryDaniel R. Griffiths0Taylor M. Jenkins1Caroline P. Addington2Sarah E. Stabenfeldt3Jonathan Lifshitz4BARROW Neurological Institute at Phoenix Children’s Hospital Phoenix AZ USADepartment of Child Health University of Arizona College of Medicine ‐ Phoenix Phoenix AZ USASchool of Biological and Health Systems Engineering, Ira A. Fulton Schools of Engineering Arizona State University Tempe AZ USASchool of Biological and Health Systems Engineering, Ira A. Fulton Schools of Engineering Arizona State University Tempe AZ USABARROW Neurological Institute at Phoenix Children’s Hospital Phoenix AZ USAAbstract Introduction The extracellular matrix (ECM) provides structural support for neuronal, glial, and vascular components of the brain, and regulates intercellular signaling required for cellular morphogenesis, differentiation and homeostasis. We hypothesize that the pathophysiology of diffuse brain injury impacts the ECM in a multi‐dimensional way across brain regions and over time, which could facilitate damage and repair processes. Methods Experimental diffuse TBI was induced in male Sprague‐Dawley rats (325–375 g) by midline fluid percussion injury (FPI); uninjured sham rats serve as controls. Tissue from the cortex, thalamus, and hippocampus was collected at 15 min, 1, 2, 6, and 18 hr postinjury as well as 1, 3, 7, and 14 days postinjury. All samples were quantified by Western blot for glycoproteins: fibronectin, laminin, reelin, and tenascin‐C. Band intensities were normalized to sham and relative to β‐actin. Results In the cortex, fibronectin decreased significantly at 15 min, 1 hr, and 2 hr postinjury, while tenascin‐C decreased significantly at 7 and 14 days postinjury. In the thalamus, reelin decreased significantly at 2 hr, 3 and 14 days postinjury. In the hippocampus, tenascin‐C increased significantly at 15 min and 7 days postinjury. Conclusion Acute changes in the levels of these glycoproteins suggest involvement in circuit dismantling, whereas postacute levels may indicate a restorative or regenerative response associated with recovery from TBI.https://doi.org/10.1002/brb3.1767extracellular matrixprimary somatosensory barrel cortexproteinS1BFtraumatic brain injuryventral posterior medial |
| spellingShingle | Daniel R. Griffiths Taylor M. Jenkins Caroline P. Addington Sarah E. Stabenfeldt Jonathan Lifshitz Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury Brain and Behavior extracellular matrix primary somatosensory barrel cortex protein S1BF traumatic brain injury ventral posterior medial |
| title | Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury |
| title_full | Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury |
| title_fullStr | Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury |
| title_full_unstemmed | Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury |
| title_short | Extracellular matrix proteins are time‐dependent and regional‐specific markers in experimental diffuse brain injury |
| title_sort | extracellular matrix proteins are time dependent and regional specific markers in experimental diffuse brain injury |
| topic | extracellular matrix primary somatosensory barrel cortex protein S1BF traumatic brain injury ventral posterior medial |
| url | https://doi.org/10.1002/brb3.1767 |
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