Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor
The method of Fe3O4 magnetic nanoparticle synthesis by co-precipitation, modification by 3-aminopropylsilane and conjugation with pH-(low)-insertion peptide (pHLIP) is reported. The characterization of nanoparticles by scanning electron microscopy, transmission electron microscopy, Fourier-transform...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340919314180 |
| _version_ | 1828375128629575680 |
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| author | Alexandra G. Pershina Olga Ya Brikunova Natalya A. Perekucha Alexander M. Demin Oleg B. Shevelev Dina Malkeyeva Elena Kiseleva Artem S. Minin Larisa A. Kostikova Ivan V. Stepanov Dmitriy K. Kuznetsov Vladimir Ya Shur Victor P. Krasnov |
| author_facet | Alexandra G. Pershina Olga Ya Brikunova Natalya A. Perekucha Alexander M. Demin Oleg B. Shevelev Dina Malkeyeva Elena Kiseleva Artem S. Minin Larisa A. Kostikova Ivan V. Stepanov Dmitriy K. Kuznetsov Vladimir Ya Shur Victor P. Krasnov |
| author_sort | Alexandra G. Pershina |
| collection | DOAJ |
| description | The method of Fe3O4 magnetic nanoparticle synthesis by co-precipitation, modification by 3-aminopropylsilane and conjugation with pH-(low)-insertion peptide (pHLIP) is reported. The characterization of nanoparticles by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, elemental and thermogravimetric analyses as well as dynamic light scattering and z-potential measurements is provided. The effect of nanoparticles on the viability of mouse and human peripheral blood mononuclear cells is tested by flow cytometry. The experimental details of nanoparticle administration to tumor-bearing mice, magnetic resonance imaging scanning as well as subsequent tumor sample collection and their processing for transmission electron microscopy, inductively coupled plasma atomic emission spectroscopy, histological and immunohistochemical analyses are described. Biodistribution of the nanoparticles in mice and blood serum analysis data for experimental animals are given. The data are useful for an experiment workflow design and for the development of theranostic systems based on magnetic nanoparticles. Keywords: pHLIP, Iron oxide magnetic nanoparticle, Nanoparticle characterization, Tumor, Biodistribution, Cytotoxicity |
| first_indexed | 2024-04-14T07:43:28Z |
| format | Article |
| id | doaj.art-766c106041614a0c8cafa6f4ad735534 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-14T07:43:28Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-766c106041614a0c8cafa6f4ad7355342022-12-22T02:05:26ZengElsevierData in Brief2352-34092020-04-0129Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumorAlexandra G. Pershina0Olga Ya Brikunova1Natalya A. Perekucha2Alexander M. Demin3Oleg B. Shevelev4Dina Malkeyeva5Elena Kiseleva6Artem S. Minin7Larisa A. Kostikova8Ivan V. Stepanov9Dmitriy K. Kuznetsov10Vladimir Ya Shur11Victor P. Krasnov12Siberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, Russia; Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 30, Lenin Ave., Tomsk, 634050, Russia; Corresponding author. Siberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, Russia.Siberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, RussiaSiberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, RussiaPostovsky Institute of Organic Synthesis UB RAS, 22, S. Kovalevskaya St., 620990, Yekaterinburg, RussiaInstitute of Cytology and Genetics SB RAS, 10, Lavrentiev Ave., 630090, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, 10, Lavrentiev Ave., 630090, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, 10, Lavrentiev Ave., 630090, Novosibirsk, RussiaMiheev Institute of Metal Physics UB RAS, 18, S. Kovalevskaya St., 620990, Yekaterinburg, RussiaResearch School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, 30, Lenin Ave., Tomsk, 634050, RussiaSiberian State Medical University, 2, Moskovsky trakt, 634050, Tomsk, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, 51, Lenin Ave., 620000, Yekaterinburg, RussiaInstitute of Natural Sciences and Mathematics, Ural Federal University, 51, Lenin Ave., 620000, Yekaterinburg, RussiaPostovsky Institute of Organic Synthesis UB RAS, 22, S. Kovalevskaya St., 620990, Yekaterinburg, RussiaThe method of Fe3O4 magnetic nanoparticle synthesis by co-precipitation, modification by 3-aminopropylsilane and conjugation with pH-(low)-insertion peptide (pHLIP) is reported. The characterization of nanoparticles by scanning electron microscopy, transmission electron microscopy, Fourier-transform infrared spectroscopy, elemental and thermogravimetric analyses as well as dynamic light scattering and z-potential measurements is provided. The effect of nanoparticles on the viability of mouse and human peripheral blood mononuclear cells is tested by flow cytometry. The experimental details of nanoparticle administration to tumor-bearing mice, magnetic resonance imaging scanning as well as subsequent tumor sample collection and their processing for transmission electron microscopy, inductively coupled plasma atomic emission spectroscopy, histological and immunohistochemical analyses are described. Biodistribution of the nanoparticles in mice and blood serum analysis data for experimental animals are given. The data are useful for an experiment workflow design and for the development of theranostic systems based on magnetic nanoparticles. Keywords: pHLIP, Iron oxide magnetic nanoparticle, Nanoparticle characterization, Tumor, Biodistribution, Cytotoxicityhttp://www.sciencedirect.com/science/article/pii/S2352340919314180 |
| spellingShingle | Alexandra G. Pershina Olga Ya Brikunova Natalya A. Perekucha Alexander M. Demin Oleg B. Shevelev Dina Malkeyeva Elena Kiseleva Artem S. Minin Larisa A. Kostikova Ivan V. Stepanov Dmitriy K. Kuznetsov Vladimir Ya Shur Victor P. Krasnov Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor Data in Brief |
| title | Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor |
| title_full | Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor |
| title_fullStr | Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor |
| title_full_unstemmed | Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor |
| title_short | Supporting data and methods for the characterization of iron oxide nanoparticles conjugated with pH-(low)-insertion peptide, testing their cytotoxicity and analyses of biodistribution in SCID mice bearing MDA-MB231 tumor |
| title_sort | supporting data and methods for the characterization of iron oxide nanoparticles conjugated with ph low insertion peptide testing their cytotoxicity and analyses of biodistribution in scid mice bearing mda mb231 tumor |
| url | http://www.sciencedirect.com/science/article/pii/S2352340919314180 |
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