Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide

The excessive use of chemical pesticides has led to pest resistance and the accumulation of residues. Pest resistance occurs when pests become immune to pesticides and require higher doses or stronger pesticides for control. This study aims to identify the optimal solvent for dissolving secondary me...

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Main Authors: Arifan Fahmi, Sapatra Enrico, Fibrilia Sekhar, Fatimah Siti, Nugraheni Arwinda, Prasetyo Alif, Januwardani Sri
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:E3S Web of Conferences
Subjects:
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/85/e3sconf_icenis2023_01020.pdf
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author Arifan Fahmi
Sapatra Enrico
Fibrilia Sekhar
Fatimah Siti
Nugraheni Arwinda
Prasetyo Alif
Januwardani Sri
author_facet Arifan Fahmi
Sapatra Enrico
Fibrilia Sekhar
Fatimah Siti
Nugraheni Arwinda
Prasetyo Alif
Januwardani Sri
author_sort Arifan Fahmi
collection DOAJ
description The excessive use of chemical pesticides has led to pest resistance and the accumulation of residues. Pest resistance occurs when pests become immune to pesticides and require higher doses or stronger pesticides for control. This study aims to identify the optimal solvent for dissolving secondary metabolite compounds that selectively and safely kill or inhibit the growth of plant pests. The biopesticide used is derived from melinjo bark extract through the process of soxhlet extraction using methanol and N-hexane solvents. The study employed a two-factor factorial design with three variables. The findings revealed that methanol solvent was more effective in dissolving phenolic compounds, resulting in a phenol content of 34.23 mg/equivalent Gallic Acid. The optimal solvent ratio was achieved at a ratio of 600 ml. The highest yield, reaching 56.27%, was obtained when using methanol as the solvent. Moreover, the pest mortality rate was found to be 100% at the specified concentration.
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spelling doaj.art-766d3cd3a1a7484596333eb0689230e82024-01-26T10:28:00ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014480102010.1051/e3sconf/202344801020e3sconf_icenis2023_01020Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of BiopesticideArifan Fahmi0Sapatra Enrico1Fibrilia Sekhar2Fatimah Siti3Nugraheni Arwinda4Prasetyo Alif5Januwardani Sri6Industrial Chemical Engineering, Vocational School, Diponegoro UniversityIndustrial Chemical Engineering, Vocational School, Diponegoro UniversityIndustrial Chemical Engineering, Vocational School, Diponegoro UniversityFaculty of Public Health, Diponegoro UniversityFaculty of Medicine, Diponegoro UniversityIndustrial Chemical Engineering, Vocational School, Diponegoro UniversityIndustrial Chemical Engineering, Vocational School, Diponegoro UniversityThe excessive use of chemical pesticides has led to pest resistance and the accumulation of residues. Pest resistance occurs when pests become immune to pesticides and require higher doses or stronger pesticides for control. This study aims to identify the optimal solvent for dissolving secondary metabolite compounds that selectively and safely kill or inhibit the growth of plant pests. The biopesticide used is derived from melinjo bark extract through the process of soxhlet extraction using methanol and N-hexane solvents. The study employed a two-factor factorial design with three variables. The findings revealed that methanol solvent was more effective in dissolving phenolic compounds, resulting in a phenol content of 34.23 mg/equivalent Gallic Acid. The optimal solvent ratio was achieved at a ratio of 600 ml. The highest yield, reaching 56.27%, was obtained when using methanol as the solvent. Moreover, the pest mortality rate was found to be 100% at the specified concentration.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/85/e3sconf_icenis2023_01020.pdfbiopesticidesn-hexane
spellingShingle Arifan Fahmi
Sapatra Enrico
Fibrilia Sekhar
Fatimah Siti
Nugraheni Arwinda
Prasetyo Alif
Januwardani Sri
Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide
E3S Web of Conferences
biopesticides
n-hexane
title Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide
title_full Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide
title_fullStr Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide
title_full_unstemmed Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide
title_short Optimization of N-hexane and Methanol Solvents on Total Phenol Levels of Gnetum gnemon Peel Extract in the Production of Biopesticide
title_sort optimization of n hexane and methanol solvents on total phenol levels of gnetum gnemon peel extract in the production of biopesticide
topic biopesticides
n-hexane
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/85/e3sconf_icenis2023_01020.pdf
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