Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells
Abstract Background Environmental factors, such as oxidative stress, have the potential to modify the epigenetic landscape of cells. We have previously shown that DNA methyltransferase (DNMT) activity can be inhibited by sublethal doses of hydrogen peroxide (H2O2). However, site-specific changes in...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-03-01
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| Series: | Epigenetics & Chromatin |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13072-021-00388-6 |
| _version_ | 1818738596273717248 |
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| author | Annika R. Seddon Yusmiati Liau Paul E. Pace Allison L. Miller Andrew B. Das Martin A. Kennedy Mark B. Hampton Aaron J. Stevens |
| author_facet | Annika R. Seddon Yusmiati Liau Paul E. Pace Allison L. Miller Andrew B. Das Martin A. Kennedy Mark B. Hampton Aaron J. Stevens |
| author_sort | Annika R. Seddon |
| collection | DOAJ |
| description | Abstract Background Environmental factors, such as oxidative stress, have the potential to modify the epigenetic landscape of cells. We have previously shown that DNA methyltransferase (DNMT) activity can be inhibited by sublethal doses of hydrogen peroxide (H2O2). However, site-specific changes in DNA methylation and the reversibility of any changes have not been explored. Using bead chip array technology, differential methylation was assessed in Jurkat T-lymphoma cells following exposure to H2O2. Results Sublethal H2O2 exposure was associated with an initial genome-wide decrease in DNA methylation in replicating cells, which was largely corrected 72 h later. However, some alterations were conserved through subsequent cycles of cell division. Significant changes to the variability of DNA methylation were also observed both globally and at the site-specific level. Conclusions This research indicates that increased exposure to H2O2 can result in long-term alterations to DNA methylation patterns, providing a mechanism for environmental factors to have prolonged impact on gene expression. |
| first_indexed | 2024-12-18T01:11:27Z |
| format | Article |
| id | doaj.art-76705e9ea37444d39a7a32b5c3689805 |
| institution | Directory Open Access Journal |
| issn | 1756-8935 |
| language | English |
| last_indexed | 2024-12-18T01:11:27Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Epigenetics & Chromatin |
| spelling | doaj.art-76705e9ea37444d39a7a32b5c36898052022-12-21T21:26:04ZengBMCEpigenetics & Chromatin1756-89352021-03-0114111610.1186/s13072-021-00388-6Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cellsAnnika R. Seddon0Yusmiati Liau1Paul E. Pace2Allison L. Miller3Andrew B. Das4Martin A. Kennedy5Mark B. Hampton6Aaron J. Stevens7Department of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoDepartment of Pathology and Biomedical Science, University of OtagoAbstract Background Environmental factors, such as oxidative stress, have the potential to modify the epigenetic landscape of cells. We have previously shown that DNA methyltransferase (DNMT) activity can be inhibited by sublethal doses of hydrogen peroxide (H2O2). However, site-specific changes in DNA methylation and the reversibility of any changes have not been explored. Using bead chip array technology, differential methylation was assessed in Jurkat T-lymphoma cells following exposure to H2O2. Results Sublethal H2O2 exposure was associated with an initial genome-wide decrease in DNA methylation in replicating cells, which was largely corrected 72 h later. However, some alterations were conserved through subsequent cycles of cell division. Significant changes to the variability of DNA methylation were also observed both globally and at the site-specific level. Conclusions This research indicates that increased exposure to H2O2 can result in long-term alterations to DNA methylation patterns, providing a mechanism for environmental factors to have prolonged impact on gene expression.https://doi.org/10.1186/s13072-021-00388-6DNA methylationDNA methyltransferaseEpigeneticsOxidative stressHydrogen peroxideCancer |
| spellingShingle | Annika R. Seddon Yusmiati Liau Paul E. Pace Allison L. Miller Andrew B. Das Martin A. Kennedy Mark B. Hampton Aaron J. Stevens Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells Epigenetics & Chromatin DNA methylation DNA methyltransferase Epigenetics Oxidative stress Hydrogen peroxide Cancer |
| title | Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells |
| title_full | Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells |
| title_fullStr | Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells |
| title_full_unstemmed | Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells |
| title_short | Genome-wide impact of hydrogen peroxide on maintenance DNA methylation in replicating cells |
| title_sort | genome wide impact of hydrogen peroxide on maintenance dna methylation in replicating cells |
| topic | DNA methylation DNA methyltransferase Epigenetics Oxidative stress Hydrogen peroxide Cancer |
| url | https://doi.org/10.1186/s13072-021-00388-6 |
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