VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis
Background: Hypovitaminosis D is a public health problem due to its implications for various diseases. Vitamin D has numerous functions, such as modulating the metabolism of cellular tissues, and it is expressed through the vitamin D receptor (VDR) gene that may influence gene expression modulation,...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/16/3565 |
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| author | Vanessa Rosa Retamoso Fernanda Barbisan Graziele Meira Moro Patricia Maurer Débora Vasquez Rubio Lauren Flores Viera dos Santos Lyana Berro Feijóo Matias Nunes Frizzo Ivana Beatrice Mânica da Cruz Vanusa Manfredini Ana Letícia Vargas Barcelos Jacqueline da Costa Escobar Piccoli |
| author_facet | Vanessa Rosa Retamoso Fernanda Barbisan Graziele Meira Moro Patricia Maurer Débora Vasquez Rubio Lauren Flores Viera dos Santos Lyana Berro Feijóo Matias Nunes Frizzo Ivana Beatrice Mânica da Cruz Vanusa Manfredini Ana Letícia Vargas Barcelos Jacqueline da Costa Escobar Piccoli |
| author_sort | Vanessa Rosa Retamoso |
| collection | DOAJ |
| description | Background: Hypovitaminosis D is a public health problem due to its implications for various diseases. Vitamin D has numerous functions, such as modulating the metabolism of cellular tissues, and it is expressed through the vitamin D receptor (VDR) gene that may influence gene expression modulation, which plays an important role in vitamin D metabolism. Objective: To evaluate the effect of the genotypes of BsmI single nucleotide polymorphism (SNP) of the VDR gene on VDR, SOD2, and CYP24A1 gene expression in individuals with low serum vitamin D levels. Methods: This was a cross-sectional analytical study. After signing the informed consent form, individuals were invited to participate and answered a structured questionnaire with identification data. Blood was collected for biochemical analysis, and vitamin D was measured by chemiluminescence; BsmI polymorphism was determined using real-time polymerase chain reaction (PCR) assays with TaqMan allelic discrimination, and gene expression was conducted by qRT-PCR using QuantiFast SYBR<sup>®</sup> Green PCR Master Mix. Data were analyzed using the SPSS 20.0 software, and differences were considered significant at <i>p</i> < 0.05. Results: 98 individuals with vitamin D ≤ 20 ng/dL were evaluated, and the BsmI SNP of the VDR gene showed CYP24A1 overexpression and low SOD2 expression. Conclusion: BsmI SNP of the VDR gene can modulate the expression of the genes evaluated without interfering with serum levels. |
| first_indexed | 2024-03-10T23:40:36Z |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T23:40:36Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-7677f6480c694f7589691945c8bd8d602023-11-19T02:29:50ZengMDPI AGNutrients2072-66432023-08-011516356510.3390/nu15163565VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with HypovitaminosisVanessa Rosa Retamoso0Fernanda Barbisan1Graziele Meira Moro2Patricia Maurer3Débora Vasquez Rubio4Lauren Flores Viera dos Santos5Lyana Berro Feijóo6Matias Nunes Frizzo7Ivana Beatrice Mânica da Cruz8Vanusa Manfredini9Ana Letícia Vargas Barcelos10Jacqueline da Costa Escobar Piccoli11Postgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilPharmacy Department and Post Graduation in Gerontology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, BrazilPharmacy Department and Post Graduation in Gerontology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, BrazilPostgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilPostgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilPhysiotherapy Course, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruguaiana 97508-000, RS, BrazilPostgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilDepartment of Life Sciences, Northwest Regional University (Unijui), R. do Comércio, 3000-Universitário, Ijuí 98700-000, RS, BrazilPharmacy Department and Post Graduation in Gerontology, Federal University of Santa Maria (UFSM), Santa Maria 97105-900, RS, BrazilPostgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilPostgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilPostgraduate Program in Biochemistry, Federal University of Pampa e Campus Uruguaiana, BR 472-Km 592-Mailbox 118, Uruuguaiana 97508-000, RS, BrazilBackground: Hypovitaminosis D is a public health problem due to its implications for various diseases. Vitamin D has numerous functions, such as modulating the metabolism of cellular tissues, and it is expressed through the vitamin D receptor (VDR) gene that may influence gene expression modulation, which plays an important role in vitamin D metabolism. Objective: To evaluate the effect of the genotypes of BsmI single nucleotide polymorphism (SNP) of the VDR gene on VDR, SOD2, and CYP24A1 gene expression in individuals with low serum vitamin D levels. Methods: This was a cross-sectional analytical study. After signing the informed consent form, individuals were invited to participate and answered a structured questionnaire with identification data. Blood was collected for biochemical analysis, and vitamin D was measured by chemiluminescence; BsmI polymorphism was determined using real-time polymerase chain reaction (PCR) assays with TaqMan allelic discrimination, and gene expression was conducted by qRT-PCR using QuantiFast SYBR<sup>®</sup> Green PCR Master Mix. Data were analyzed using the SPSS 20.0 software, and differences were considered significant at <i>p</i> < 0.05. Results: 98 individuals with vitamin D ≤ 20 ng/dL were evaluated, and the BsmI SNP of the VDR gene showed CYP24A1 overexpression and low SOD2 expression. Conclusion: BsmI SNP of the VDR gene can modulate the expression of the genes evaluated without interfering with serum levels.https://www.mdpi.com/2072-6643/15/16/3565vitamin Dhypovitaminosiscytochrome P450 (CYP)SOD2 |
| spellingShingle | Vanessa Rosa Retamoso Fernanda Barbisan Graziele Meira Moro Patricia Maurer Débora Vasquez Rubio Lauren Flores Viera dos Santos Lyana Berro Feijóo Matias Nunes Frizzo Ivana Beatrice Mânica da Cruz Vanusa Manfredini Ana Letícia Vargas Barcelos Jacqueline da Costa Escobar Piccoli VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis Nutrients vitamin D hypovitaminosis cytochrome P450 (CYP) SOD2 |
| title | VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis |
| title_full | VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis |
| title_fullStr | VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis |
| title_full_unstemmed | VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis |
| title_short | VDR, SOD-2, and CYP24A1 Gene Expression in Different Genotypes of BsmI SNP of the Vitamin D Receptor Gene in Individuals with Hypovitaminosis |
| title_sort | vdr sod 2 and cyp24a1 gene expression in different genotypes of bsmi snp of the vitamin d receptor gene in individuals with hypovitaminosis |
| topic | vitamin D hypovitaminosis cytochrome P450 (CYP) SOD2 |
| url | https://www.mdpi.com/2072-6643/15/16/3565 |
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